细胞核

Objective To evaluate the effects of glomerular cell proliferation and apoptosis in experimental glomerulosclerosis.

用RT-PCR方法和免疫组化法检测增殖细胞核抗原的表达，原位检测肾小球细胞凋亡指数。

Methods Cell cultures were harvested and stained with haematoxylin and eosin,to analyze cellular metaphase morphology.

收获细胞经苏木素和伊红染色，观察分裂中期细胞核形态，计算核极分裂象细胞百分比。

Cultured cells were harvested and stained with haematoxylin and eosin to analyze cellular metaphase morphology.

收获细胞经苏木素和伊红染色，观察分裂中期细胞核形态，计算核多极分裂像细胞百分比。

Methods Cell cultures were harvested and stained with haematoxylin and eosin,to analyze cellular metaphase morphology.

收获细胞经苏木素和伊红染色，观察分裂中期细胞核形态，计算核多极分裂象细胞百分比。

Observation of cultured cells stained by haematoxylin and eosin: It was shown that the cells presented round or ellipse. There was little cytoplasm, presenting acidophily. Nucleus was big and round, single in the center, deep stained.

培养细胞苏木精-伊红染色观察结果：可见细胞圆形或椭圆形，胞质少呈嗜酸性，细胞核大而圆，单个居中，着色深。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

TGF-βbinds to a heteromeric complex of type I and type II receptors. The constitutively active type II receptor phosphorylates and activates the type I receptor which phosphorylates Smad2 and Smad3 (receptor-associated Smads). Receptor-associated Smads bind the common mediator Smad4 and are translocated to the nucleus, where they associate with DNA-binding cofactors to modulategene transcription.

TGF-β与其效应细胞表面的Ⅱ型受体TβRⅡ和Ⅰ型受体TβRⅠ形成的异源二聚体结合，相继激活TβRⅡ和TβRⅠ，活化的TβRⅠ再进一步活化Smad2和Smad3，磷酸化的Smad2/3必须与Smad4结合形成复合物，才能进入细胞核，调节靶基因的转录[6]。

When human histiocytic lymphoma cell line, U937 cells, was treated with cadmium for 12 hours, evidence of apoptotic features, including change in nuclear morphology, DNA fragmentation, formation of DNA ladder in agarose gel electrophoresis, and phosphatidylserine externalization, were obtained.

人淋巴瘤细胞株（U937）暴露于镉12小时后，细胞呈现典型的凋亡特征，包括细胞核形态学改变、DNA梯形条带的形成、及细胞膜磷脂酰丝氨酸外翻转。

The measured morphological parameters of cells in all groups were in accordance with histomorphological standard, hich indicated that the database was established successfully.

所测量的各组细胞核的形态参数与组织形态学标准相符，建库成功。

The gland secreted in a holocrine fashion,It had two types of cells:type A and type B.

腺体进行全浆分泌，有A、B两种细胞类型，A型细胞具常染色质，粗面内质网和线粒体丰富，B型细胞具异染色质，细胞核固缩。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。