细胞核

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Specialized dosimetric models and computational methods are needed.

肿瘤及周围正常组织、靶区中辐射敏感的细胞和细胞核的吸收剂量及其分布是辐射效应分析时重要的物理量，其估算需要特殊的剂量学模型和计算方法。

The effects of different treatments on electrofusion of pig 2 cell embryos were studied with Hubei white pigs as experimental animals.

哺乳动物配子和早期胚胎细胞融合技术在研究卵子和早期胚胎发育中的细胞质相互作用，制作4倍体胚胎，杂种细胞构建和胚胎细胞核移植等方面有重要意义。

Objective To study the effects of different parameters for electrofusion on the activation and early development of mouse embryos reconstructed by cumulus cell nuclear transfer, and explore optimal parameters for electrofusion.

目的研究不同电融合条件对来源于小鼠卵丘细胞核移植重组胚的融合和早期发育的影响，寻找最佳电融合参数。

Cell nucleus/transplantation ; electrofusion ; oocytes ; embryos

细胞核/移植；电融合；卵母细胞；胚胎

In a draft preface to the recommendations, discuss ed at the 17 May meeting, Shapiro suggested that the panel had found a broad consensus that it would be "morally unacceptable to att empt to create a human child by adult nuclea r cloning".

参考译文在 5 月 17 日的会议上所讨论的这份建议书的序言草案中，夏皮罗提出，专家组已经达成广泛共识，那就是&试图通过成人细胞核克隆来制造人类幼儿的做法在道德上是不可接受的&。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Objective To investigate the expression of survivin, PTEN and PCNA protein in endometrial carcinoma and its clinical significance.

目的 观察survivin、PTEN、增殖细胞核抗原在子宫内膜癌中的表达及临床意义。

Documents reportedβ-catenin transfered into endonuclear in basal cell and II type epithelial cell of damaged bronchus in patients who were diagnosed IPF.

文献报道IPF病人受损支气管基底细胞和损伤部位Ⅱ型上皮细胞中发现β-catenin向细胞核内转移。

We used maize seedlings (Zea mays L.) and tobacco (Nicotiana plumbaginjfolia L.) as material. In the first section, we have investigated cold- shock-induced chilling, drought, heat and salt stress resistance in maize seedlings. In the second section, by modifying the Ca~ and CaM levels, we have tried to establish the involvement of calcium messenger system in the cold-shock-induced cross adaptation. In the third section, we have tried to establish a transgenic tobacco system expression CaMICaMIBP genes, so that the others can use it to investigate the process of physiology and biochemistry which endonuclear and cytosolic calmodulin have taken part in.

以玉米幼苗和烟草为实验材料，本文第一部分通过将玉米幼苗进行短期冷驯化后对冷胁迫、干旱胁迫、热胁迫及盐胁迫的影响，试图证明玉米幼苗中交叉适应现象的存在及其机理；第二部分通过外源修改Ca~(2+)和CaM水平，研究了钙信使系统在交叉适应现象中的可能作用；第三部分通过建立诱导性表达CaM/CaMBP转基因烟草体系，便于研究受细胞质CaM和细胞核CaM调控的各种生理生化过程。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。