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By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变;流式细胞术周期分析显示在G1期峰前存在一个凋亡峰(20.6%);核琼脂糖凝胶(DNA 15 g.L-1)电泳呈梯状。

Among the six cytologically atypical samples, four were in the malignant range, two were in the benign limits, and these were proved by histopathology.

6例疑诊恶性胸腔积液患者胸腔积液细胞核内AgNOR数目和形态,4例具有恶性特征,2例在良性范围,为组织病理所证实。

Under fluorescence microscope, cytolymph could emit red fluorescence with Rhodamine, and cellular nucleus could emit blue fluorescence with DAPI, accompanying myofilament structure.

免疫细胞化学检测:荧光显微镜下,罗丹明染色细胞浆呈红色荧光,并可见肌丝结构;DAPI染色细胞核呈蓝色荧光。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中,充满病毒粒体的细胞呈条带状分布,呈现出明显的侵染和增殖中心,在充满病毒粒体的细胞周围,相邻细胞有不同程度的病变,叶绿体膜系统消失,部分叶绿体中含有病毒颖粒,线粒体残体及细胞质中有分散或聚集的病毒粒体,细胞核中未见病毒粒体,离侵染中心较远的细胞中亚细胞结构较完整;RDV浙江分离株侵染的水稻叶片中,部分细胞充满病毒粒体,无明显的条带状分布,其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

Although ERβ was also seen in these cells,there was irregularity of its distribution.Weak to moderate cytoplastic staining of ERβ in epithelial cells of lobules and ducts were fonud.

ERβ在小叶上皮细胞和导管上皮细胞的细胞核内检测到,但是ERβ在上皮细胞中的分布并无规律,而且在细胞浆内也可以检测到弱到中等强度的染色。

The study was purposed to investigate the effect of deguelin on expression of nucleoporin 98(nup98) in leukemia K562 cells.

本研究观察鱼藤素对白血病细胞株K562细胞核孔蛋白nup98表达的影响。

Effects of deguelin on the regulation of nucleoporins and nucleophosmin in Jurkat leukemia cells in vitroObjective: To study the antileukemia effects of deguelin on leukemia cells, and explore the possible mechanisms to provide more theories for clinical application.

第一部分鱼藤素对Jurkat白血病细胞核孔蛋白与核磷蛋白的调节作用目的:研究鱼藤素对白血病细胞增殖和凋亡的影响,并探讨其可能的作用机理,为其临床应用提供理论基础。

Results: After 24h exposure to rhBMP-2, the expression of PCNA in HFCL cells decreased 0.65 fold by densimetric scanning. Observed after staining with the Wright-Gimsa, the numbers of cells with mitosis imaging decreased, but the morphologic change of cells did not happen. The HFCL cell numbers in S phsae decreased while in G1 phase increased, the cell cycle was arrested at G1 phase HFCL cells did not differentiate to myelocytic or mononucleate cells.

结果:经过rhBMP-2作用24h后,HFCL细胞PCNA表达下降,经灰度扫描下降幅度为0.65倍;瑞氏-吉姆萨染色后发现细胞核分裂像减少,分裂指数下降,但细胞形态没有变化;HFCL细胞S期中的细胞数量减少,G1期数量增多,出现G1期阻滞;rhBMP-2作用没有使HFCL细胞向髓系或单核方向分化的趋势。

In the more advanced fungi, especially the Basidiomycota, nuclei may fuse some considerable time after fusion of hyphae of different mating strains, forming a dikaryon.

在较高等的真菌中,尤其在担子菌门中,不同类型的菌丝融合后细胞核需要较长的时间进行融合,从而形成双核体。

The karyotype analysis shows that the majority of the chromosomes of root-tip cell were monosomic(2n=20Ⅱ+1=41),only minority were disomic and nullisomic(about1%).

进行根尖细胞核型分析,发现大部分为单体(2n=20Ⅱ+1Ⅰ=41),在其中仅看到极少数二体和缺体,比例不到1%。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。