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细胞核

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By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现,CaM和CaLP的亚细胞定位是截然不同的:CaM分布于外套膜上皮细胞的细胞核与细胞质中,而CaLP却仅分布于外套膜上皮细胞的细胞质中。

Among these features are: absence of the nuclear rotation and development of the centriolar complex and the initial region of the axoneme in a position directly perpendicular to the basal pole of the nucleus and presence of numerous thick anchoring fibrils connecting the proximal centriole and the proximal part of the distal centriole to the nucleus.

其特点主要是:生精过程中不发生细胞核的旋转,中心粒复合体和轴丝起始段直接发生在核的基底面垂直线上,有无数的粗的固定纤维将近端中心粒和远端中心粒的近侧部连接到细胞核上。

In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中,同源框基因islet-1表达产物(ISL-1)免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内,ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等,另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布,如,屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

We propose that APP may have the function of anchor to catch ANKS1B to prevent into the nucleus, the cleavage of AICD is very important to the translocation of ANKS1B, and AICD and ANKS1B may co-transport into nucleus.

推测APP蛋白似乎可以抓住ANKS1B,避免ANKS1B进入细胞核,所以AICD的切割对於ANKS1B的转位很重要;也许AICD会和ANKS1B一起进入细胞核

In this paper an automated cell nucleus segmentation method is proposed based on a novel fuzzy growing snake model for color cell images from esophageal smears.

针对细胞核之间经常出现重叠聚堆的现象,提出了一种新的基于模糊膨胀模型的细胞核轮廓提取方法。

Results:The AgNOR results of malignant pleural effusion were obviously higher than those in tuberculosis pleural effusion(P.01).There were many dispersion,congregation and commix in the morphological study of AgNOR particles in malignant pleural effusion,but single form in tuberculosis pleural effusions.

结果:癌性积液组AgNOR的10项参数测定指标均与良性组有显著性差异(P.01),并且癌性胸腔积液组癌细胞核内AgNOR颗粒以弥散型、聚集型和混合型为主,而结核性积液组细胞核内AgNOR颗粒以单一型为主。

The ultrastructural changes during the development of sieve element companion cell complex (SE/CC complex) in Populus deltoides were investigated using electronic microscopy. The result showed that SE and CC tome from the same secondary phloem mother cell. The development of sieve element went through three stages, i. e. immature, mature, and degenerate stage. In immature stages the sieve element presented radial expansion, cell wall thickening, and production of sieve plastid and P-protein. The cytoplasma selectivly autolysed in mature stage. At the same time, karyoplasm was dispersed, and nuclear envelope was not clear, or karyoplasm was disorgnized, and nuclear envelope was clear. The karyopalsm finally turned into P-protein. In degenerate stage, the cytoplasm completely disappeared, and the function was lost.

利用电镜技术研究美洲黑杨次生韧皮部筛管和伴胞发育过程中细胞超微结构的动态变化,观察到筛管和伴胞由同一个次生韧皮部衍生细胞分裂分化形成筛管约发育历经未成熟期、成熟期和衰退期的变化未成熟期筛管分子表现为细胞的径向扩展、壁的增厚、筛管质体和P-蛋白质的产生;成熟期筛管分子细胞组分发生选择性自溶,细胞核核质弥散状,核膜不清晰,或核膜清晰而核物质降解,最后细胞核完全解体转化为P-蛋白质类的物质;衰退期筛管分子细胞质完全解体消失,失去功能。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料,从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用,并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

ConclusionThe formation and diminishment of the manchette is in step with the condensation and elongation of the nucleus of the spermatid. Both the structural and positional changes of the manchette coincide with the changes of the nucleus. These results imply that manchette might play an important role in mouse spermiogenesis.

Manchette结构的形成和消失与精子细胞核的浓缩及延伸同步,其形态变化和位置改变与精子细胞核的形态学变化相吻合,在小鼠精子细胞变态成形过程中具有重要意义。

Result's standard: statistics management:use chi-square test,exact probabilities in 2×2 table and correlation analysis.

结果判定:p16蛋白的阳性表达为细胞核,cyclinD1蛋白阳性表达为细胞核;参照Shimizu等的判断标准。

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