细胞核

Results showed that β-catenin was positive expression in stellate reticulum,inner dental epithelium,and dental sac at cup stage,in inner dental epithelium,dental papilla cell,and cell nucleus at early bell stage,in cervical loop and cusp cell membrane at late bell stage,and in ameloblast and odontoblast cell at amelogenesis stage.

结果表明，帽状期在星网层、内釉上皮、牙囊均为细胞核阳性表达；钟状早期在内釉上皮、牙乳头细胞核阳性表达；钟状晚期在颈环、牙尖细胞膜阳性表达；成釉期早期在成釉质细胞、成牙本质细胞阳性表达；成釉期晚期均阴性表达。

In conclusion, we detailedly investigated the variation of membrane system (mainly plasmalemma, amyloplast envelope, ER) with the potassium permanganate fixative method, nucleus and organelles with routinely fixative method, and their functions with cytochemical method (localization of phosphatases and Ca〓).

综上所述，本文运用高锰酸钾固定法研究了膜系统（主要指质膜、淀粉质体被膜、内质网）的变化，运用常规固定制样研究了细胞核和细胞器的变化，采用细胞化学方法（Ca〓和磷酸水解酶的定位）探索了细胞器和细胞核的功能。

The results indicated that the erythropoietic/granulopoietic ratio was markedly increased with megaloblastic morphology in all stage of erythrocyte.

结果表明：2例CDAⅠ型患者骨髓幼红细胞比例增加，各阶段细胞存在不同程度巨幼样变；原红细胞核不规则，早幼或中幼红细胞可见奶酪核，约半数晚幼红细胞有核溶解和破碎现象，晚幼红细胞核损伤有时伴胞质溶解，细胞间染色质桥少见；各阶段红细胞核膜和内质网同时出现不同程度溶解。

The Ca〓 in nuclear oscillated before GVBD and became stronger when Ca〓 transferred from extranuclear into the nuclear.

在GVBD发生前，细胞核内出现有规律的强度越来越大的波动现象，细胞核周围的Ca〓不断向细胞核内波动传递。

After treated with the samples, the MGC-803 cells are dyed with the fluorescence dye Hoechst 33258. Observed by the fluorescence microscope, the dense dyeing granulometric and nubby blue fluorescence can be seen in the nucleus of MGC-803 treated with AK-9 and AK-11, the nucleus of some cells smash to small fragments.

采用荧光染料Hoechst 33258对经初筛活性样品处理的MGC-803细胞染色，荧光显微镜观察，发现经AK-9及AK-11发酵液处理的细胞，其细胞核内可见浓染致密的颗粒或块状荧光，细胞核发生固缩、碎裂，而对照细胞的细胞核呈均匀弥散荧光，表明这2株菌株可能产生具有诱导细胞凋亡的活性物质。

Establishing 6 fuzzy sets such as cells with increscent karyon, cells with deeply dyed karyon, cells whose area rates of the karyon and cytoplasm are excessive and so on and 6 membership functions.The paper partitions the sample space furtherly into 4 nomarl pattern, and realizes the recognition of the cancer cells in pleural fluid.

建立了诸如细胞核增大的细胞、细胞核深染的细胞、细胞核不圆的细胞等6个模糊集合和与之相应的6个隶属函数，并根据模糊集合的运算法则将胸水细胞样本空间进一步划分为4个标准模式，实现了胸水癌细胞的识别。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

After estimating the ellipse parameters for each cell nucleus, the image point is mapped into several fuzzy domains which measure tinctorial uniformity and positional proximity of the pixel to the nucleus.

结合细胞核的椭圆边界信息，将图像数据映射到反映与细胞核颜色和位置关系的多个模糊域；基于这些模糊映射关系，建立了一种新的主动轮廓模型进行细胞核轮廓跟踪。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。