细胞核

In response to TCDD exposure, AhR nuclear translocator forms a heterodimer with the aryl hydrocarbon receptor after the ligand-dependent translocation of AhR to the nucleus, and eventually the complex binds to the xenobiotic response elements, which promote transcription of several specific genes, such as CYP1A1.However, the diverse effects of TCDD could not be simply explained by the AhR pathway.

对於戴奥辛的毒性机制，以往的研究认为与芳香基碳氢化合物受体（arylhydrocarbon receptor－AhR）的活化有关；TCDD 活化了AhR，被活化的AhR 从细胞质转移到细胞核内，在细胞核与另一个蛋白质AhR nuclear translocator形成二聚体，并与DNA 上的xenobiotic regulatory element结合，诱发某些基因（如：CYP1A1）的转译与表现；不过，单从AhR的代谢途径并不能解释所有生物上的毒性现象。

A large number of necleocapsids were seen in the nuclei and cytoplasm at 12 h post infection, and the peak was at 24 h, but enveloped virions were rarely seen in the nuclei and cytoplasm.

结果表明：RNC在感染VZVJI6h后核内可见散在的核衣壳，12h后细胞核和细胞质内核衣壳明显增多，2 4h达高峰，而细胞核和细胞质内的成熟病毒颗粒较少见。

The cell and cell nucleus are taken to be concentric spheroids.

细胞由细胞膜、细胞质和细胞核组成，细胞核位于细胞的中央，周围被细胞质包围。

Our study showed that EGFprotein was mainly localized in the cell plasma of distal tubules and Henle'sloops and EGF mRNA was distributed not only in the nucleuses of above sitesbut also in a few nucleuses of interstitial cells and glomerulus cells.

本研究表明EGF蛋白主要位于髓袢和远曲小管细胞胞浆，而EGFmRNA除了位于上述部位的细胞核中外，在少数间质细胞、肾小球细胞细胞核内也有少量表达。

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后，同A组相比：E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高，在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著，在卵泡、黄体中阳性产物表达均较A组显著降低，在子宫中表达较A组变化不明显；同B组相比：E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高，在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低；同C组相比：E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高，在室周核、黄体中均显著降低；同D组相比：E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高，在视上核、弓状核显著降低，在其它部位表达变化差异不显著。

When aborted rats were injected with 10 IU/g IFN-γin group D, compared with group A, expression of TNF-αinmmuopositive substance in the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami were highly increased, conversely, the ones in the nucleus pre-opticus suprachiasmaticus, neurohypophsis, adenohypophysis, ovary were obviously decreased, the change in the uterus was not remarkable; Compared with group B, expression of TNF-αin the arcuatus hypothalami was obviously enhanced, while in the nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, neurohypophsis, pars intermedia, ovary, the results were on the contrary, the uterus had no significantly variation; Compared with group C, expression of TNF-αin the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and pars intermedia had an obviously risen trend, however, the variation in the nucleus pre-opticus suprachiasmaticus, nucleus periventricularis hypothalami, neurohypophsis, pars intermedia, follicle and corpus luteum was completely opposite, uterus had no obviously change.

外源腹腔注射10 IU/g IFN-γ后，同A组相比：D组TNF-α免疫阳性产物在下丘脑视前内侧核、视上核、视前大细胞核、室周核、室旁核、弓状核显著增高，在视交叉上核显著降低，在其它核团较A组无显著差异，在神经垂体、垂体前叶、卵巢中阳性产物表达均较A组显著降低，在子宫中的表达较A组变化不明显；同B组相比：D组TNF-α免疫阳性产物在弓状核显著增高，在视交叉上核、视前大细胞核、室周核、室旁核、腹内侧核、背内侧核、神经垂体、垂体中间部、卵巢各部阳性物质表达均显著降低，在子宫表达变化不显著；同C组相比：D组下丘脑视前内侧核、视上核、室旁核、弓状核、垂体前叶与中间部均显著增高，在视交叉上核、室周核、神经垂体、卵泡、黄体中均显著降低，子宫中表达变化不明显。

After the exposure, the retinas were examined with light and electron microscope. results: under light microscope, irregular nucleoplasm and unclear border of nucleus in the ganglion cell layer were found in the experimental groups of 2 h, 12 h and 24 h. these changes were also found in the inner nucleus layer. under the electron microscope, swollen mitochondria and vacuolized endoplasmic reticulums were found in all the layers of retina in the groups of 2 h, 12 h and 24 h, most obvious in the group of 24 h. in the group of 72 h, only lightly swollen mitochondria and dilated endoplasmic reticulums were found in the ganglion cell layer of retina.

结果： 光镜：实验组中2，12及24 h组均可见视网膜神经节细胞层内细胞核出现核质不均匀，核边界不清，内核层细胞核也有同样改变；电镜：实验组中2，12及24 h组视网膜各层均出现线粒体肿大，内质网空泡样变，以24 h组最为严重，72 h组仅见视网膜神经节细胞层出现轻度线粒体肿大，内质网扩张。

The results showed that the ZNF191fu and ZNF434fu functioned as transcription repressors while the ZNF396fu can only weakly suppress the transcription activity of the reporter, and the ZNF397fu and ZNF447fu showed no signanificant capability of transcription regulation.

而转录因子发挥作用需要进入到细胞核中，因此，我们进一步分析了这些基因的不同剪接本在细胞内定位的情况，结果显示我们所检测的5个成员的nf剪接本都能被相应的fu剪接本带入细胞核内，并且这种作用是通过SCAN结构域所介导的。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

A novel method used for segmentation of nucleus is proposed: filter the origin image with the median filter in both horizontal and vertical direction, which has a fairly wide window size, in order to reduce the infection of the noise and impurity; in both direction, use the threshold combined with the technology of finding the peak in local area to detect the real nucleus edge, and thus minimize the non-nucleus edge; also in both direction, let the border growing respectively using the type information; thereafter, weed the short border and link the border together to segment the nucleus, exploiting the convex character and border type.

提出一种新的细胞核分割方法：对图像使用水平和竖直方向的中值滤波，使用较大的尺度，尽量的消除杂质、噪声影响；在两个方向分别使用采用阈值限制以及局部最大值的方法检验边界，使非细胞核边界被剔除；在两个方向分别利用边界点的类型进行边界生长；边界生长完毕以后，剔除短边界，用两个方向获得的边界进行连接，充分利用其凸凹性和边界类型，完成分割。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷