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More materil as took from resected specimen is helpful for finding more small cell carcinoma,there is poor prognosis in small cell carcinoma concomitant with squamous cell carcinoma compared with squamous cell carcinoma alone,it is suggested that primary small cell carcinoma of esophagus might be derived from a totipotentiality primitive cell in the basal region of the squamous mucosa of the esophagus.

对手术切除标本足够取材有利于发现肿瘤的异源性成分;小细胞癌合并鳞癌者,预后较单纯鳞癌者差;本文结果支持食管小细胞癌起源于原始多潜能干细胞的观点。

Cytokine and adhesion molecule are critical for the reception of uterine endomembrane and the invading of trophectoderm.

其中,细胞因子和黏附分子在着床期子宫内膜的容受性和滋养外胚层细胞的侵入中起了重要作用。

Results: The positive unit of survivin expression intensity in trophocyte cytoplasm and karyon was subsequently increased in following order: normal early gestation villi, hydatidiform mole, invasive hydatidiform mole and choriocarcinoma.

结果:survivin在滋养细胞细胞核及细胞浆的表达强度PU值依下列顺序增加:正常早孕组、葡萄胎组、侵蚀性葡萄胎组及绒癌组,各组间的表达强度差异有统计学意义(P.05)。

The calf intestinal epithelial cells in vitro were selected to evaluate the trophic actions of insulin, IGF-J and peptic and tryptic hydrolysates of bovine colostrum. The cell proliferation, glucose absorption and specific activity of cell membrane enzymes were investigated.

为研究乳源性生长因子对动物胃肠道生长发育的作用,本试验以离体犊牛小肠上皮细胞为试验对象,研究了胰岛素、胰岛素样生长因子Ⅰ和酶解牛初乳对细胞增殖、葡萄糖吸收和细胞膜酶活性的影响。

Methods The present study included twelve undialyzed ESRD patients with anemia.The sera from the uremic patients were added to CFU-E and BFU-E culture in the concentrations ranging from 1.25% to 5%.In vitro CFU-E and BFU-E growth in the presence of sera from ESRD patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.

选择12例尿毒症肾性贫血未透析患者为试验组,另选正常健康体检者12例为正常对照组,将实验组、正常对照组血清分别稀释成不同终浓度,采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养,对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。

It is unsensible to buy ESCs from other labrotaries, because of the pluripotency of ESCs would be affected by passage number and freeze thawing. So we need to built steadily mouse embryonic stem cells-derived and tested system.

因为ES细胞的多能性会受到多次传代以及冻融的影响,因此需要在本实验室条件下建立起ES细胞稳定的分离培养和鉴定体系。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白,主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运,并维持一定细胞内外的离子梯度,我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增,限制性酶切分析扩增产物,并进行荧光测序,对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析,发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg,Fc。

After lock out of the 5LO gene by homologous recombination in embryonic stem cells, 5LO deficient-mice can develop normally and stay healthy, with a selective opposition to certain inflammatory insults. The cellular distribution of 5LO in unstimulated cells among different cell types is different; it can present in the cytosol, the nucleus, or both.

采用同源重组方式敲除胚胎干细胞中的5-脂氧合酶基因,小鼠能正常生长和发育,但对某些炎症损伤的反应性与正常小鼠不同。5-脂氧合酶在不同的细胞中表现出不同的亚细胞定位,分布于胞质或胞核,或两者都有。

The maximum induction occurred at 12 h, at which transcripts were 0.34 fold of that in urediniospore. The minimum accumulation occurred at 96 h, whose transcript was only 0.02 fold of that in urediniospore.

PsCdc2可能通过调控条锈菌的细胞周期循环参与了侵染前期初生菌丝生长和吸器母细胞的形成,与条锈菌的致病性相关。

Vacuolar H+_ATPase showed higher correlation with plant salinity tolerance because of its contribution to salt ion compartmentation.

本文综述了高等植物细胞ATPase在盐胁迫下的活性变化及其调控机制。V型H+_ATPase与细胞离子区隔化和植物抗盐性密切相关。

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