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Recombinant apoE〓 protein could inhibit the death of the neurocyte cell of mice brain induced by beta amyloidal peptide 25-35 fragment and the death of the SK-N-SH-neuroblastoma cell induced by beta amyloidal peptide 25-35 fragment and 1-40 fragment.

重组apoE〓蛋白对Aβ 25—35多肽诱导的胎鼠大脑皮质神经细胞凋亡及Aβ25-35和A β1-40多肽诱导的神经成纤维胶质瘤细胞生存力下降均具有明显的拮抗作用,表明apoE对神经退化性疾病中的细胞死亡具有拮抗作用。

Using the worksheets,"Analogism Tests of Cell-Related Curriculum", TIWM and "Cell Structure and Physiological phenomenon Achievement test". In consideration of the evaluation result, we try to explore the correspond proportions which the students of experimental group that is achieves by the education of conceptual change.

使用学习单、「细胞相关课程类比测验」、TIWM和「细胞构造及生理现象成就测验」等评测学生概念改变之成效,并辅以诠释性分析,探究实验组有多少比例的学生其另有概念之改变,是经由概念改变教学而达成。

Angiectasis, permeability were increased , inflammatory cell were transuded; The cardiac muscle injury conditions after hypoxia 4 h were similar with hypoxia 2h group, appeared lipid droplet, implied the oxidative metabolism of lipid got obstacle started from then; The cardiac muscle injuried conditions were got little improvement in hypoxia 6 and 8 hours team, mitochondria were increased and aggregatived, normal cell junction, sarcomere contract weaked; in hypoxia 10h group , mitochondria were increased and aggregatived obviously, a few glycogenosome were assembled, transverse tubule were expanded, all the changes may be the adaption of Gansu zokors in the hypoxia tolerance process to 4% hypoxia extent gradually.

在4%严重低氧耐受过程中,甘肃鼢鼠心肌超微结构在2h组出现血管扩张,通透性增加,有炎细胞渗出,异染色质边集;4h组心肌损伤状况与2h组接近,出现脂滴,提示脂类的氧化代谢在此时开始发生障碍,6和8h组心肌损伤状况逐渐好转,线粒体聚集增多,细胞连接正常,肌小节收缩较前有所减弱;10组线粒体增多聚集,线粒体脊增粗更为明显,少量糖原颗粒聚集,脂滴较前有所减少,横小管扩张增大变粗,出现了与能量代谢有关的结构适应性变化。

Methods: Tissue of rat ovary was obtained from 30 healthy sexually mature non-mated femal SD rats. The rats were divided into two groups (oestrus group and anoestrus group). The expression of α-SMA and vimentin were detected and evaluated by immunohistochemistry and image analysis system in the theca cells of different type follicles.

分别取动情期和非动情期大鼠卵巢,运用免疫组化方法检测肌成纤维细胞的特征性标记和波形蛋白分别在这两组中各级卵泡卵泡膜细胞上的表达情况,并进行图像分析。

Recent study have found that statins have lipid-lowering effect, multi-effect, including antioxygen, anti-inflammatory, immunomodulatory, lower levels of cytokines, improve endothelial cell function and prevention of thrombosis, reduce inflammation reactions.

近来的研究发现他汀类药物除了降脂作用外,还具有多效性,包括抗氧化,抗炎,免疫调节,降低细胞因子水平,提高内皮细胞功能和防止血栓形成,降低炎性反应等。

Recent study have found that statins have lipid-lowering effect, multi-effect, including antioxygen, anti-inflammatory, immunomodulatory, lower levels of cytokines, improve endothelial cell function and prevention of thrombosis, reduce inflammation reactions.

近来地研究发现他汀类药物除了降脂作用外,还具有多效性,包括抗氧化,抗炎,免疫调节,降低细胞因子水平,提高内皮细胞功能和防止血栓形成,降低炎性反应等。

METHODS: The total RNA was obtained by TRIzol from an antiricin neutralizing monoclonal antibody hybridoma cell named 4C13 and heavychain and lightchain variable fragments were amplified by overlap PCR and accepted by Genbank of NCBI. VH and VL were linked by3 with a direction of VHlinkerVL and cloned to pCDNA3.1 vector expression system.

用TRIzol提取抗ricin中和性单抗杂交瘤细胞4C13总RNA,扩增其轻、重链可变区基因,登陆GenBank;用Linker3将VH和VL连接,用重叠延伸PCR克隆入真核表达载体pCDNA 3.1,用脂质体转染293T细胞,对瞬时表达产物进行生物学活性检测。

Primary culture of granulosa cells from ovarian antral follicles of gonadotropin-primed immature rats was used.

本研究是利用初级卵巢颗粒细胞培养系统进行实验,细胞是取自性促素所诱发未成熟大鼠之中大型空腔卵泡。

The slurry was inoculated on a rocking bed and continued for about 4 weeks, then arsanilic acid was determined spectrophotometrically (450 nm) and the genotoxicity changes of arsanilic acid on earthworm coelomocytes were tested with comet assay (single cell gel electrophoresis test, SCGE) in 0, 1, 2, 3, 4 weeks, respectively.

结果表明,阿散酸低浓度时在泥浆体系中能被较快降解,500mg/L的降解率达到了25%;蚯蚓体腔细胞彗星试验表明,所有降解液均具有遗传毒性,可导致蚯蚓体腔细胞DNA明显损伤,与对照相比具有显著性差异(P.05)。

Results: All transplant livers which survived more than 30 days developed moderate to severe manifestations of graft infiltration, ductular damage, obliterative arteriopathy and liver fibrosis.

结果:移植后30天始,B,C组移植肝出现从中等到严重程度的泡沫细胞阻塞性动脉病变,胆管损伤或消失,细胞浸润,移植肝纤维化。

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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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