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To make it clear, using multilaser flow cytometry, molecular biologic technology and laser confocal microscope scan technology, we fixed eyes on mitochondrial permeability transition pores, mitochondrial transmembrane potential and caspases-3 (cysteinyl aspartate-specific proteinases) which are playing key roles of mitochondria. Meanwhile, the research also discussed the characteristics of Δψm of cells in cut specimenfrom esophageal carcinoma, adjacent tissue of esophageal carcinoma and the normal esophageal tissue.

为探讨食管癌细胞凋亡过程中线粒体所起的具体作用,本课题采用流式细胞检测、分子生物学和激光共聚焦扫描技术等手段,研究了食管癌细胞凋亡中,线粒体所起关键作用的环节—线粒体通透性转变孔(Mitochondrion Permeability Transition Pore,MPTP)、线粒体跨膜电位ΔΨm和线粒体释放反应中的Caspase-3(Cysteine protease-3);同时研究了食管癌手术切除标本中癌灶、癌旁和切端三处组织的细胞线粒体跨膜电位的特性。

This papers explore the correlation between the thickness of blade、cuticula、epidermis cells、fence form、sponge form,the ratio of the fence form and sponge form,the layer of the fence form,the length of cell and the yield,suitable system,resistance of the blade anatomical structure by paraffin section method and observing in optical microscope using tea varieties in Guangxi,such as the No.1 of Guilv, and provide a scientific basis for tea scientific research and production.

本文主要对近年来广西选育成的桂绿1号等新品种品系通过石蜡切片法,并采用光学显微镜观察叶片解剖结构特征,探索叶片解剖结构中的叶片、角质层、表皮细胞、栅栏组织、海绵组织等厚度及栅栏组织与海绵组织厚度的比值、栅栏组织层数、细胞长度等与产量、适制性、抗逆性之间的相关性,为茶叶科研、生产利用提供科学依据。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后,通过与ONYX-015(E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒,美国ONYX生化制药公司研制)、wtAd5进行对比,利用病毒增殖实验和细胞生长抑制实验,验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用;利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异,揭示了腺病毒肿瘤靶向性的机制;利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株,直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Objective: To investigate the expression of Cytotoxin associated gene A and Vacuolating cytotoxin A,gastric epithelial cell proliferation and apoptosis of chronic superficial gastritis with helicobacter pylori infection and Spleen - Stomach Damp-Heat .

目的:观察幽门螺杆菌感染慢性浅表性胃炎脾胃湿热证患者血清中CagA、VacA的表达及胃粘膜上皮细胞增殖和细胞凋亡情况。

The data suggest that Huayusanjie Tablet can prevent the formation and development of PVR in vitro and in vivo. Its mechanism concern with factors described below: lowering the concentration of PDGF, decreasing the expression of ICAM-1 and VCAM-1 in proliferative membrane and vascular endothelial cell, directly inhibiting proliferation of RPE in vitro.

化瘀散结片能有效防止PVR的形成与发展,其作用机理与减少玻璃体中血小板源性生长因子浓度,降低视网膜增生膜上和血管内皮细胞表面ICAM-1、VCAM-1的表达,以及直接抑制体外培养的视网膜色素上皮细胞增生有关。

Results By using optical microscope, the neurocytes of hippocampus in experimental group were observed to denaturalize, tumefy, destroy and disappear.

点燃癫痫鼠海马区损伤越重,胶质细胞GFAP免疫反应性越强,通过检测GFAP可以推测星形胶质细胞与癫痫后脑损伤的程度。

OBJECTIVE: To investigate the directional differentiation of BMSCs into hepatocytes with alcohol-hepatocirrhosis patient serum.

目的:探讨在酒精性肝硬化患者血清诱导条件下,人骨髓间充质干细胞向肝细胞样细胞的定向分化情况。

On the one hand,Magnetic Polymer Microsphere could covalent active materials—enzymes,cells and antibodies;on the other hand,they could show strong magnetic responsibility for their directional movement in the magnetic field.

一方面磁性聚合物微球可通过共价键来结合酶、细胞和抗体等生物活性物质;另一方面因其具有"在外界磁场存在下能定向运动"这一特殊性能,可对外加磁场表现出强烈的磁响应性,因此它被用作酶、细胞、药物等的载体,广泛地应用到生物医学、细胞学和生物工程等领域。

That PI and the percentage of the cells in s-phase of the borderline/malignant and heteroploid/aneuploid tumors were higher ted to higher mortality and relapse rate as well as the corresponding risk. Because of the changable characters and dissociative phenomenon of the tumor, the measurement of PI and the percentage of cells in s-phase may be disturbed in few of diploid tumor and in part of double DNA stem ones. Therefore other paramet- ers should be added to analyse before making any conclu- sion.

交界性和恶性、异倍体或非整倍体肿瘤的PI和S期细胞百分率较高,病死加复发率和相对危险性增加;由于肿瘤的异质性和"分离现象",对于少数2倍体肿瘤和部分双DNA干系肿瘤PI和S期细胞百分率的测定易受干扰,故应结合其它参数综合分析。

Five (22%) of the 24 T-cell tumors expressed βF1, 8 (35%) of the 23 tumors were positive for EBER, and 20 (95%) of the 21 tumors were clonal for T-cell receptor.

那24例T细胞淋巴瘤中有5例(22%)表达βF1;有23例做了EBER检测,其中8例(35%)阳性;有21例做了T细胞受体基因检测,其中20例(95%)为多克隆性重排。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。