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P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞,P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%,20%,50%,80%,100%)的胶原/透明质酸支架中,在无血清培养液中三维培养。2周后,用SO染色和免疫组织化学染色分析,观察硫酸软骨素和Ⅱ型胶原合成情况。

In details, BMSCs in 20% hyaluronan/collagen I scaffolds could perform a hyaline-cartilaginous phenotype when they were expanded with less than 3 passages and cultured in a chondrogenic medium, and CCs did better than BMSCs in chondrogenesis with high passage number; however, ADAS just showed a minor chondrogenesis even in a pellet culture.

结果提示,在同样条件下骨髓间充质干细胞、软骨细胞较脂肪源性成熟基质细胞有更好的成软骨性能。

Objective:To study the adipogenous potential of endothelial cells derived from SD rat adipose tissue.

目的:研究脂肪源性内皮细胞向脂肪细胞的分化潜力和过程。

It also is related to bone marrow structural damage resulting from tumor invasion,large proliferation of granulation or fibrous tissue,which often affect hemopoiesis.Leukemoid reaction occurred in the majority of malignancies is myeloid leukemia response,among which some are.neutropenia,and even agranulocytosis and increasion or decreasion of platelets.These are all have close relationship with marrow invasion of malignant tumor,immune reaction of the body and chemotherapy as well as radiotherapy.

亦与骨髓被恶性肿瘤细胞浸润、肉芽和纤维组织大量增生,使骨髓结构破坏,影响红系造血有关;绝大多数恶性肿瘤并发的类白血病反应是粒细胞性的,部分患者白细胞减少,甚至粒细胞缺乏,以及血小板增高或减少,均与恶性肿瘤细胞骨髓浸润、机体的免疫反应、放化疗有关。

Under 100mmol/L NaCl stress,ailanthuscompartmentalized Na+ in vacuole,while under 200mmol/L treatment,the Na+ content incytoplasm was higher than vacuole.the ailanthus cells took up large amount of Cl- and didnot concentrate Cl- in vacuole.

在根和叶细胞中Na+通过液泡膜的转移和细胞K+含量稳定性的维持能力的不同是银杏和臭椿耐盐性差异的重要原因。

Juvenile bronchial asthma is a severe disease which is harmful to children. It characterized of whoop , dyspnea , chest stuffiness and cough time after time. It is caused by the EOS, T-cell and so on which lead the chronic inflammation of respiratory air-way. Asthma often outbreak or aggravate at nighttime and early morning.

支气管哮喘简称哮喘,是由嗜酸性粒细胞、肥大细胞和T淋巴细胞等多种炎性细胞参与的气道慢性炎症,使易感者对各种激发因子具有气道高反应性,并可引起气道缩窄,表现为反复发作的喘息、呼吸困难、胸闷或咳嗽等症状,常在夜间和清晨发作、加剧,多数患儿可经治疗或自行缓解。

In an attempt to evaluate the safety and efficacy of Healon in trabeculectomy, 20 eyes with Healon and 20 eyes without Healon injection after trabeculectomy are compared. Trabeculectomy using Healon will not induce transient rise of intraocular pressure. By filling the anterior chamber with Healon, a tamponing effect results which will maintain a deep chamber and reduce complications such as flat anterior chamber, choroidal detachment and striate keratopathy in the early postoperative period. In long-term evaluation there is no difference in pressure control, conjunctival scarring and corneal endothelial cell loss between Healon group and Non-Healon group.关键词 trabeculectomy , flat anterior chamber , choroidal detachment , intraocular pressure

为了评估Healen在小梁切除术中之疗效及安全性,选20位接受小梁切除术者,同时注射Healon於前房为实验对象,另20位接受传统小梁切除术为对照组,发现使用Healon并不会引起一过性眼压上升的现象,Healon注入前房,产生填塞作用,最大的好处是可以维持前房深度,减少术后合并症如前房消失,脉络膜剥离及角膜水肿等,然长期追踪,对於降眼压的幅度,不因使用Healon而可达到低眼压状态,也不会减少结合膜瘢痕化而促进房水引流,然Healon系一无毒,无炎性,无抗原性高黏稠性的物质,不因使用而发生炎性反应,同时有保护角膜内层细胞的功能。

Objective: To investigate the pathogenesis of allergic rhinitis at cell level by determing basophil histamine releasability in guinea-pigs and attempt to find an objective way for the diagnosis of allergic rhinitis.

目的:通过对变应性鼻炎豚鼠的嗜碱粒细胞释放组胺能力的测定,从细胞水平探讨变应性鼻炎的发病机制,并期望为变应性鼻炎的诊断指标提供一种客观手段。

Collagen has many useful features such as low antigenicity, controllable biodegradation, biological absorbable properties, haemostatic properties, ability to promote cellular growth and attachment.,which make it an excellent naturally occurring biomaterial, it has a wide application in medicinal industry and clinical medicine.

胶原蛋白是一种天然存在的具有很高利用价值的生物物质,其特有的极低的抗原性、可生物降解性、生物吸收性、止血性能和促进细胞生长的功能,使得近年来在医药工业和医学临床的研究和应用日益广泛。

We observed the variation of TBIL in obstructive jaundice rats and then made the bile duct ligation model. We also investigated the changed regularity of pulmonary capillary membrane permeability in hyperbilirubinemia, utilized the immunohistochemistry method to study the expression of pro-inflammatory cytokines in lung tissue. To search for the reason about the change of pulmonary capillary membrane permeability when the rats in BDL.

本研究应用了胆总管结扎的梗阻性黄疸大鼠模型,研究了高胆红素血症大鼠肺泡毛细血管膜通透性的变化,并利用免疫组化等方法研究了致炎细胞因子在肺组织内表达的改变,以探索肝功能障碍时大鼠肺泡毛细血管膜通透性发生变化的可能原因。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。