细胞性

Objective To study the mechanism in which the personality, copying style influenced survival time by changing immunity function in the patients with gastric cancer Methods The Minnesota Multiphase Personality Inventory, coping style questionnaires and immunity function were measured in patients with gastric cancer on late stage, gastric cancer patients with longer survival time, and normal control Results Psychopathic deviate, social introversion subscale scores of MMPI in groupⅡ were significantly lower than that in the other two groups ( P 05) Statistical difference were found between groupⅠand groupⅡ in problem saving factor and help seeking factor of CSQ ( P 05) Natural killer cell level in groupⅡ was significantly higher than in groupⅠ( P 05) There was negative correlation between the NKC and depression, psychopathic deviate, paranoia, psychasthenia, schizophrenia subscale scores of MMPI, there was positive correlation between NKC and SP, coping type in patients with gastric cancer Conclusion The gastric cancer patients with longer survival time are more extroversive and have more mature coping styles Their NKC level is relatively higher The results may be advantageous to improve the prognosis of gastric cancer

作者中文名：王健；邹义壮；唐丽丽；李杰；张东；金山；刘娟摘要：目的研究胃癌患者的生存期与个性特征、应付方式等心理因素，以及免疫功能的关系。方法对胃癌晚期患者、胃癌较长生存期患者及正常健康人分别进行明尼苏达多相个性测验、应对方式问卷、免疫功能测试，并做相关分析。结果(1)MMPI：胃癌Ⅱ组人格障碍评分(42.0±10.5)及社会内向评分(42.2±11.6)，低于其他两组[胃癌Ⅰ组为(49.3±11.2)分和(48.3±9.4)分，对照组为(48.6±10.9)分和(39.9±11.5)分；P均＜0.05]；(2)CSQ：解决问题和求助因子分，胃癌Ⅰ组[分别为(0.68±0.27)分和(0.42±0.18)分]均低于胃癌Ⅱ组[分别为(0.82±0.18)分和(0.65±0.21)分；P＜0.05和P＜0.01]，胃癌Ⅱ组更多采取成熟的应对方式；(3)自然杀伤细胞：胃癌Ⅱ组水平(21.4±9.2)高于胃癌Ⅰ组[(14.2±8.8)；P＜0.05]；而NK细胞水平与MMPI的抑郁(r=-0.35)、人格障碍(r=-0.39)、偏执(r=-0.29)、精神衰弱(r=-0.30)及精神分裂(r=-0.30)等量表呈负相关，与CSQ的解决问题因子(r=0.35)及应付方式类型(r=0.34)呈正相关。结论与胃癌Ⅰ组比较，胃癌Ⅱ组更为外向，负性情绪较少，更多采取成熟的应对方式，其NK细胞水平较高，对胃癌的预后产生了积极的影响。

The first passage cells were initially pured by enzyme digestion, then pured by enzyme digestion and repeated strapping alternatively in the second course of transferation and inoculation, the third period cultured cells showed typical clonali growth and were long fusiform fibroid cells with short growth cycle.

首次传代时应用胰酶消化法进行成纤维细胞的初次纯化，第2次转种时先后应用酶消化法和反复贴壁法再次进行细胞纯化，第3代以后基本可获得呈典型克隆性生长、长梭形纤维样细胞，生长周期短。

This is usually a physical response to infection or inflammation because monocytes or tissue macrophages are activated by various stimuli to liberate various cytokines with pyrogenic activity.

这往往是由于人体对感染及炎症的生理性反应，在其中，单核细胞或组织巨噬细胞被繁多的刺激物激活而释放多种致热性细胞活素。

In PN group, the light microscopic findings included inflammation cells infiltration and hepatic steatosis, and electron microscopy showed vacuole change in cytosol and rarefactive microvillus in micro-bile duct. Conclusion PN-associated liver dysfunction was related with hepatocyte oxidative injury and apoptosis.

PN肝脏损伤机制可能是PN某些配方成分应用中，脂质过氧化导致肝细胞氧化损伤，同时损伤线粒体膜，促使线粒体膜通透性增加，释放细胞色素C等凋亡相关因子，激发caspase-3等级联反应，产生肝细胞凋亡，导致肝损伤。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50% only accounted for 1.87%, 10%＜ MPRA ＜50% for 17.62% and MPRA ＜10% 15.44%,respectively.

结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%＜MPRA＞10%以及MPRA＜10%的分别占17.62%和15.44%。

Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50%only accounted for 1.87%, 10%MPRA50%for 17.62%and MPRA10%15.44%,respectively.Conclusion Before renal transplantation, the detection of MPRA level could predict the occurrence of rejection in renal transplantation.

结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%10%以及MPRA结论在肾移植术前检测患者MPRA水平，对肾移植后的排斥反应能起到预测目的。

Immunohistochemical assay were performed with HGV NS5 McAb. Results One of four samples showed positive staining, some of which located in cytoplasm and some of which in cytonucleus and cytomembrane. The number of positive staining cells are not more. More of them disrtibuted scatteredly, some of them located.

结果 1例呈阳性染色，光镜观察显示：特异染色大部分定位于肝细胞胞浆中，部分有核着色和膜型染色，呈黄色或棕黄色颗粒状，阳性细胞数量少，大多散在分布，部分呈灶性分布；阳性细胞周围可见较多淋巴细胞浸润。

Cells in medullary carcinomas of thyroid gland can appear very pleomorphic or spindled, but there usually are better differentiated cells with neuroendocrine fetaures with or without amyloid, calcification, and giant cell reaction.

甲状腺髓样癌的细胞可能显示明显的多形性和梭形细胞样，但通常有分化较好的细胞伴神经内分泌特征，伴或不伴淀粉样变、钙化和巨细胞反应。

The histopathology showed that the tumors were rich in cells arranged in fascicles with bipolar and spindled processes. The nuclei showed minor pleomorphism. A few tumor cells were centered on the vessels with a nuclear-free zone that was characteristic of ependymoma.

病理组织学上肿瘤富于细胞，排列成束状、交织状，细胞胞突细长双极，核异型性小，少数肿瘤细胞围绕血管周围形成室管膜瘤的无核区结构。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。