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Initial adherenced cells mostly are round or short fusiform. Division growth of adherenced cells was shown 72 hours after fully changing suspension(.2)Flow cytometry analysis of the adherenced MSCs indicated good homogenicity of passage cells. More than 95% CD34- and CD44+ demonstrated the cells we get were purified MSCs.

2对贴壁生长的MSCs进行流式细胞仪检测,提示传代的细胞均一性较好,表面抗原CD34表达阴性,CD44表达阳性的细胞均在95%以上,说明得到的是比较纯化的MSCs。

Oligoclonal expansion T cells could be detected and Vβ21 oligoclonal T cells were identified in all 3 cases.Conclusions The skew distribution and clonal expansion of TCR Vβ subfamily T cells could be found on the patients with CML.

结果 3例病人仅存在3~9个TCR Vβ亚家族T细胞,部分Vβ亚家族T细胞呈寡克隆性增殖,Vβ21的寡克隆T细胞见于全部的3例病人中。

Results ec was cultured and subcultured successfully. vascular endothelial cells show slabstone appearance enchased arrange under phase contrast microscope. the growth appearance was showed under sem. some dispersive high electron density of rod type weible palade body were seen under tem.

结果 培养出血管内皮细胞并传代,倒置显微镜下血管内皮细胞呈"鹅卵石"形态,扫描电镜显示"铺路石"样生长,透射电镜可见内皮细胞特征性w p小体,免疫组织化学鉴定ⅷ因子。

Methods 30 BALB/c mice were randomly divided into three groups, normal control group, 100, 200 mg/kg APS intraperitoneal injection groups. After one week, weight the mouse spleen, account the splenetic index. Collect the mouse bone marrow cell, induced and cultured with rmGM-CSF and rmIL-4. With inverted microscope to investigate the morphous of DC cell. The phenotypes of DC were detected by flow cytometry and the expression of the GM-CSF protein in serum was tested by ELISA.

分别设对照组及APS 100、200 mg/kg 剂量组,腹腔注射,体内诱导小鼠髓源性DC前体和未成熟DC,称量脾重,ELISA检测血清中GM-CSF表达量,倒置显微镜观察细胞生长状态,电镜检测细胞形态,流式细胞仪检测CD11c、MHC-Ⅱ类分子及协同刺激分子CD80、CD86表达水平。

The microculture tetrazolium assay was used to determine the anti-proliferative effects of As2O3 on human RCC lines.

结果:As2O3以浓度依赖方式抑制RCC-WCS细胞的体外生长,在浓度为0.5、1.0、2.0和4.0μmol/L作用96h时,As2O3对RCC-WCS细胞的抑制率分别为27.60%、30.09%、41.03%和50.77%;与未经As2O3处理的RCC-WCS细胞相比,均有显著性差异(P.01)。

Combined with self-organized theory of cell ular automata and elasto - plastic theory, the elasto-plastic cell ular automata updating rule is presented.

将细胞自动机的自组织理论与弹塑性理论相结合,推导了分析平面塑性问题的细胞自动机更新规则,采用Weibull随机分布模拟岩石类材料的非均质性,元胞单元服从简单的本构关系和一定的强度准则,建立平面弹塑性细胞自动机模型,开发了相应的数值分析软件。

If the result of the suppression of T cell infection, radiation, thymus gland dysfunction and other factors and features to reduce time, B cells, T cells as a result of the loss of control of hyperthyroidism may have a large number of autoantibodies, and cause a variety of autoimmune diseases.

如果抑制性T细胞因受感染、辐射、胸腺功能紊乱等因素的影响而功能降低时,B细胞因失去T细胞的控制而功能亢进,就可能产生大量自身抗体,并引起各种自身免疫病。

A group of acid soluble boiling stable proteinsin fig cells accumulated in alarge amount when treated by NaCl,and which was more in AH cell lines and under higher salt media,suggesting it might be an important factor in salt tolerance of fig cells.

此外,无花果细胞在盐胁迫下积累大量的酸溶热稳定蛋白质,而且AH细胞的积累量高于HO细胞,高盐度培养基中积累量高于低盐度培养基,表明可能与耐盐性有着重要关系。

Previous studies have shown thatlymphocytes from patients with malig-nant disease, when encountering abasic protein fraction isolated fromhuman glioma, display a slowingeffect to macrophage electrophoreticmobility.

对用人脑胶质瘤碱性蛋白免疫的38只豚鼠和23只对照组豚鼠进行了巨噬细胞电泳试验,其巨噬细胞电泳减缓率免疫组为6.13±1.06%,对照组为0.96±0.90%;经统计学处理,两组间有显著区别,表明GBP能激发豚鼠的细胞免疫反应性。

The selective deposition of agrin in hepatocellular carcinoma microvessels versus sinusoidal walls prompted us to investigate the utility of agrin immunohistochemistry in detecting malignant hepatocellular lesions.

Agrin选择性地沉积在肝细胞肝癌的微血管而非肝窦壁,此特点就促使我们研究应用agrin免疫组化来诊断肝细胞恶性病变的效用问题,着重于肝细胞腺癌和异型性结节,因为这两者的鉴别诊断常常很困难。

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