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The NK cytotoxicity was inhibited in RD-10 cells with high expression of MHC class Ⅰ molecules, but the γδT cells cytotoxicity was same among the three groups. Then the tumorgenicity of these tumor cells were determined in nude mice heterograft model in vivo.

动物接种肿瘤的实验结果表明,Balb/c裸鼠在接种野生型RD细胞、RD-neo细胞及RD-10细胞(低表达IL-15)后,各组小鼠肿瘤生长在体积和瘤重方面并无显著性差异。

Light microscope examination indicated that heteromorphism and karyoplasmic ratio were decreased when treated with 90μmol/L,140μmol/L,240μmol/L and 400μmol/L DADS for 48 hours in a dose and time dependent.

细胞形态学观察显示:90μmol/L,140μmol/L,240μmol/L和400μmol/L DADS处理细胞48后,与对照组细胞相比,异型性明显减少,核浆比例明显减小。

Results from whole-cell patch clamp showed: 1 BmK Ⅰ(α-like neurotoxin) was found to prolong the duration of action potential and promote the amplitude of action potential. The effects could be attributed to the selective action of BmK Ⅰ on the TTX-S Na〓 channel: slowing the inactivation process of Na〓 channel, increasing the amplitude of peak Na〓 currents; 2 BmK abT, a new-type neurotoxin with higher sequence homology to β-type scorpion neurotoxin, was found to slow the inactivation process of Na〓 channel and prolong the duration of action potential in DRG neurons.

全细胞膜片钳研究发现:1)类α型哺乳动物毒素BmK Ⅰ可延长部分DRG神经元全细胞动作电位的时程,增加动作电位的幅度,这些效应可归咎于BmK Ⅰ选择性地作用于DRG神经元TTX—S钠通道,延缓TTX—S钠电流的失活化相,峰钠电流幅度增加;2)BmK abT,一个与β型毒素同源性较高的新型蝎毒素可抑制DRG神经元全细胞钠电流失活化过程,使动作电位时程增加。

Aminoglycosides suppress termination at nonsense codons and result in functional improvement in a cystic fibrosis bronchial epithelial cell line, in muscle cells from mdx mice and in fibroblasts from individuals with Hurler syndrome.

氨基糖苷类物质可以通过抑制无意义密码子的终止作用,改善囊性纤维化的支气管上皮细胞、患肌营养不良的肌细胞和Hurler综合征病人的成纤维细胞的功能。

It was indicated that damp-heat syndrome effected the normal cell division cycle and encouraged the excess cell proliferation because of the special pathologic state such as closely relation with active inflammation, augmentation of the immune function and cell energy hypermetabolism.

推测可能是由于"湿热"这种特殊的病理状态(如与活动性炎症关系密切、免疫功能亢进、细胞内能量代谢亢进等)影响了细胞正常的分裂周期,促使细胞过度增殖。

When measuring the water permeability, people used to make the cell in the circumstance of hypotonicity. Our research has contrast the other method to make the cell in the circumstance of hyperosmotic using FluoStar Optima in CHO-V163S-AQP4、FRT-V163S-AQP1 and SMMC-7221-V163S. We has confirmed that the latter is more sensitive and accurate than the former in the process of high throughput screening AQP inhibitor drug on the first time.

在测定细胞水通透性变化时,人们通常采用给细胞造成低渗环境的方法,本研究将给细胞造成高渗环境的方法与之做了对比研究,应用FluoStar Optima荧光仪对CHO-V163S-AQP4、FRT-V163S-AQP1以及SMMC-7221-V163S进行两种方法对比测定,首次证明了,在高通量药物筛选AQP阻断剂过程中,后者较前者灵敏度更高、测定结果更准确。

The biological activity glass has the good bone conduction, the inductivity, can accelerate osteocyte multiplication, causes the osteoblasts to transform into the mature osteocytes.

生物活性玻璃具有良好的骨传导性、诱导性,能加速骨细胞的增殖,使成骨细胞转变为成熟的骨细胞。

Aim To observe changes of peroxisome proliferator activated receptor γ mRNA, ATP-binding cassette transporter A1 (ABCA1) mRNA and CD36 mRNA in foam cells which come from the peritoneal macrophage of apolipoprotein E knock out mice with giant knotweed rhizome, hawthorn and both of them, and discuss possible mechanism of anti-atherosclerosis on gene level.

目的 观察中药虎杖、山楂配伍对载脂蛋白E基因敲除小鼠巨噬细胞源性泡沫细胞内过氧化体增殖物激活型受体γ、三磷酸腺苷结合盒转运子A1及CD36 mRNA表达的影响,从基因水平探讨虎杖和山楂配伍对动脉粥样硬化泡沫细胞形成的干预机制。

It was found that theintrduction of polar phosphatidylcholine to LLA chains had largely changed thehydrophilicity of the synthesized polymers. And from the research ofendothelioid cell (ECV304) compatibility and adhesion of blood platelet, wefound that the cells adhesion of ECV304 on the films of PLLA-PC-PLLA lagedcomparing to PLLA, but then could proliferate and cover the films in total,PLLA-PC-PLLA, the same as PLLA are not cytotoxicity and ECV304 canattach and proliferate on them, and PLLA-PC-PLLA can reduce the adhesion ofblood platelet.

结果表明:由于聚乳酸中磷脂酰胆碱结构的引入,使得该材料的亲水性得到很大的改善,并且随着聚合物中磷脂结构单元比例的增高其亲水性能就越好;通过对该材料进行细胞亲和性的实验研究,发现这种材料能够使细胞锚着并支持细胞的增殖;而血小板黏附的实验结果初步显示了该材料具有较好的抗血小板黏附的性质。

Standard patch clamp methods were used to measure whole cell capacitance,zero current potential,reversal potential and potassium ion currents of Deiters′ cells in normal external solution.Equations were used to compute the relative membrane permeability ratio of Deiters′ cell to K+ and Na+ and the potassium equilibrium potential.

采用酶孵育加机械分离法分离Deiters细胞;利用膜电钳技术在全细胞模式下检测全细胞电容,记录在正常外液中的钾电流,零电流电位,反转电位,并根据相应公式计算离子通道K+与Na+的相对通透性比率及K+平衡电位。

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