细胞性

The characteristics of typical pattern of MF infection are nodular granulomas or diffuse inflammation with mixed granulomas which con-sisted of central abscesses surrounded by epithelioid and giant cells,abundant vacuole zosters or ring structures(lipid-like struc-tures) around the necrotic lesions and small granulomatous nodules.

结节性或弥漫性炎症伴混合性肉芽肿(中央为脓肿围绕着上皮样细胞及巨细胞)以及坏死灶及肉芽肿样小结节周围出现大量空泡细胞条带或环状结构为偶发分枝杆菌感染的最典型表现形式。

Results SDF-1/CXCR4 was present in the basal epithelial cells of normal conjunctiva. Of 20 pterygia cases, 17 expressed SDF-1/CXCR4 in the full thickness of the epithelium and CXCR4 was also found in the inflamed cells and vascular endothelial cells in the tissues; 3 primary pterygium cases showed the same staining pattern as that in normal conjunctiva.

结果 在正常结膜组织中SDF-1/CXCR4仅表达于基底部上皮细胞；胬肉组织中有17例，全层上皮细胞均有SDF-1/CXCR4的表达，胬肉基质层中浸润的炎性细胞、血管内皮细胞均有CXCR4的蛋白表达；3例初发性翼状胬肉表达方式与正常结膜相似。

Using immunofluorescence staining with antibodies of specific enzyme of mast celland phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning confocal microscope.

利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记；以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型；使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

Using immunofluorescence staining with antibodies of specific enzyme of mast cell and phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning cofoncal microscope.

分泌方法：利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记；以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型；使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

The Northern blot analysis showed that the mRNA of ECP 63 is expressed in embryogenic cells, globular, heart-, and torpedo-shaped embryos, but not in seedlings and non-embryogenic cells, which indicated that ECP 63 gene plays a critical role in somatic embryogenesis.

以ECP63 cDNA全长作探针的Northern分子杂交结果表明，ECP63基因在胚性细胞和不同发育时期的胚状体中高度表达，且表达量基本相等，但在幼苗和非胚性细胞中不表达。表明ECP63基因在体细胞胚胎发生方面起着重要作用。

This page provides some protocols about porcine brain tubulin, sea urchin sperm axonemes, rat liver cell cytosol and organelle fractions, organelle motility assay, tissue culture of PtK1 cells, basic protein chemistry and so on.

该网页提供了一些有关猪脑的微管蛋白、海胆的精子基因丝、大鼠肝脏细胞的细胞质和细胞器的级分、细胞器的运动性实验、PtK1细胞的组织培养、基本的蛋白化学等等方面的操作流程。

As one of the most important osmoregulation actor, firstly,Proline plays a keyrole in the osmotic potential adjustment in plant cells. Secondly, Proline can preventNaCl from damaging the structure of biomacromolecule in cells. Thirdly, the highdissolubility and non-rejection capability of Proline can enlarge the solution volumnof the cell, so the concentration of salt will be diminished in cytoplasm and the saltstress will be relieved. Forthly, the accumulation of Proline could prevent thedehydrolysis from sap cavity in cytoplasm. Fifthly, the synthesis of plant chlorophyllwill be diminished under the salt stress, but the Proline can provide request ofchlorophyll synthesis.

脯氨酸作为渗透调节剂对植物体细胞渗透势的变化起重要作用：一，在盐胁迫下，脯氨酸在植物体内是一种重要的渗透调节剂，它是一些高等植物和绿藻抗盐和抗旱的主要渗透剂之一；二，脯氨酸可以保护细胞中的生物大分子的结构，使之不被NaCl破坏，并能维持其完整的水合范围；三，脯氨酸的高度溶解性以及其对植物各种酶活性不抑制性，可以扩大细胞的溶解容积，从而降低细胞质液中盐的浓度，减轻盐的胁迫作用；四，细胞质中积累的大量脯氨酸可以防止液泡对细胞质的脱水作用；五，在盐胁迫下，植物叶绿素的合成受到抑制，叶绿素的合成需要脯氨酸。

The result of bioinformatics analysis showed that it had no remarkable homology with the known genes in GenBank. And the RSG6 gene is localized on the chromosome 8 and 10 of Oryza sativa L. with one copy for each; its homologous fragments exist also on the chromosome 4 and 12 of rice.

精细胞与二细胞花粉的差减文库中获得的在精细胞中优势表达的克隆作为探针，筛选水稻精细胞cDNA文库而得到的基因，它与已知基因没有明显的同源性，为新发现基因。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Tissue Preparation: 159 cases of paraffin sections of lung carcinomascomprised of adenocarcinoma (84 cases), squamous cell carcinoma (42 cases), smallcell lung carcinoma (10 cases), large cell carcinoma (15 cases), pleomorphic carcinoma(4 cases), and metastatic colon carcinoma (4 cases), and 86 adenocarcinomas fromsome organs other than lung, including colon (15 specimens), stomach (5 specimens),prostate (5 specimens), breast (35 specimens), uterus body (11 specimens), ovary (10specimens) and thyroid (5 specimens) were chosen from the surgical files ofYamanashi University Hospital and Kofu City Hospital.

标本来源：159例肺癌及其癌旁组织标本包括84例腺癌、42例鳞癌、10例小细胞癌、15例大细胞癌、4例多形性癌和4例肺转移性结肠癌，以及86例全身其他组织腺癌及其癌旁组织标本包括15例结肠腺癌、5例胃腺癌、5例前列腺癌、35例乳腺癌、11例子宫内膜腺癌、10例卵巢癌及5例甲状腺癌，均来自日本山梨大学附属医院和甲府市立医院外科手术标本。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。