细胞性

When pea ( Pisum sativum L.,Alaska strain) was grown in short\|day conditions,the apical bud senescence started with the transformation of the bud from vegetative to reproductive growth.DNA fragmentation was detected during the senescence using the terminal deoxynucleotidyl transferase\|mediated dUTP nick end in situ labeling method.The cell ultrastructural changes were similar to apoptosis in animal cells:the apical meristemetic cells underwent the programmed cell death.This wa...

显微、超微结构研究表明，短日照条件下豌豆顶芽的衰老过程是从营养生长锥向花芽的转化，而用DNA原位末端标记、Caspase 8WesternBlot和 14 0bpDNA片断积累的试验结果证明，转化为花芽的整个生长锥细胞发生了编程性死亡，而且其最顶端部分细胞首先发生PCD ，而顶端周围的分生组织细胞逐渐分化出花芽的各部分，但顶芽最后并没有发育成为完整的花，所有细胞就都发生PCD ，从而顶芽衰老

The pathologic changes of fungal diseases in alimentary canal were tissue necrosis, ulcer formation and pseudomembranous inflammation.

其病变为消化道组织坏死、溃疡形成及假膜性炎症；炎细胞浸润不明显，且以单核细胞浸润为主；假膜性炎中有较多的中性粒细胞渗出，可能与某些细菌的混合感染有关系。

Acupuncture and moxibustion could recover obviously the allergic rhinitis patients' symptoms and signs, enhance the patients' ability to avoid the disease recurrency.

Th1细胞因子IFN-γ水平与常年性变应性鼻炎的症状、体征呈负相关，Th2细胞因子IL-4与常年性变应性鼻炎的症状、体征呈正相关。

ResultsAfter seven days of culture, some BMSCs appeared typical morphological neuronal traits: the refractile and cone like or spherical cell bodies and long processes, expressed Nestin or NSE or GFAP.

结果 实验组诱导7 d后有部分细胞具备神经元样细胞形态，胞体锥形或圆形，有较长单极或多极的突起，均有Nestin、NSE、GFAP阳性细胞表达，且3种细胞数量差别有显著性（t=3.266~6.099，P.05）。

ResultsAfter seven days of culture, some BMSCs appeared typical morphological neuronal traits: the refractile and conelike or spherical cell bodies and long processes, expressed Nestin or NSE or GFAP.

结果 实验组诱导7 d后有部分细胞具备神经元样细胞形态，胞体锥形或圆形，有较长单极或多极的突起，均有Nestin、NSE、GFAP阳性细胞表达，且3种细胞数量差别有显著性（t=3.266~6.099，P.05）。

Results(1) Group of electroacupuncture in Shu and dense wave can significantlyreduce the degree of ankle swelling(P＜0.05, P＜0.01), which shows thatelectroacupuncture has obvious anti-inflammatory and repercussive roles;(2) Theresults of HE staining showed that group of electroacupuncture in Shuand dense wave can significantly reduce inflammatory cell infiltration,inhibit synovial cell proliferation, and decrease proliferation of granulationtissue in rats joints;(3) group of electroacupuncture in dense-wave can increase the pain thresholdof experimental rats (P＜0.05, P＜0.01), reduce the content of peripheral painmediums, such as K~+, DA and 5-HT(P＜0.05, P＜0.01), which shows thatelectroacupuncture has significant analgesic effect;(4) In the local lesion of joints, electroacupuncture can significantly reducethe content of IL-1β, IL-8, TNF-a, increase IL-4 content, decrease theexpression of COX-2mRNA, and significantly promote the expression ofHSP-70 protein in synovial tissues of acute gouty arthritis rats (P＜0.05,P＜0.01),thus restrain the occurrence of synovial tissue inflammation.

结果：(1)电针疏密波组能显著减轻踝关节肿胀度(P＜0.05，P＜0.01)，表明电针有明显的抗炎消肿作用；(2)HE染色显示：电针疏密波组可使大鼠关节炎症细胞浸润明显减轻，滑膜细胞增生明显被抑制，肉芽组织增生明显减少；(3)电针密波组能提高实验性大鼠痛阈(P＜0.05，P＜0.01)，降低外周疼痛介质K~+、DA、5-HT的含量(P＜0.05，P＜0.01)，表明电针有明显的镇痛作用；(4)在病变关节局部，电针能显著降低急性痛风性关节炎大鼠滑膜组织IL-1β、IL-8、TNF-α含量(P＜0.05，P＜0.01)，升高IL-4的含量(P＜0.05，P＜0.01)，并降低关节滑膜内COX-2mRNA的表达(P＜0.05，P＜0.01)，可促使急性痛风性关节炎大鼠滑膜内HSP-70蛋白表达显著增加(P＜0.05，P＜0.01)，从而抑止滑膜组织炎症发生。

Among the 45 neurons generating phasic firing, 8 (17.78﹪) neurons could still be induced phasic firing after treatment with 1×10-2 μg/mL SVHRP and 37 (82.22﹪) neurons had no responses to the stimulation. The AP firing of neurons was dramatically different after treatment with SVHRP (P＜0.01, n=45). Among the 7 repetitive firing neurons, all of them could only generate 1 or 0 AP instead of repetitive firing when SVHRP was applied. The number of APs was 14.57±1.00 and 0.57±0.20 before and after SVHRP treatment (P＜0.01, n=7). The AP rheobase was (75.10±8.99) pA and (119.85± 12.73) pA before and after 1 × 10-4 μg/mL SVHRP application, respectively (P＜0.01,n=8). The AP threshold was increased from (-41.17±2.15) mV to (-32.40±1.48) mV after 1×10-4 μg/mL SVHRP treatment (P＜0.01,n=8). The peak amplitude of AP was (68.49±2.33) mV for the neurons before treatment with 1×10-4 μg/mL SVHRP and (54.71±0.81)mV after treatment (P＜0.01, n=8). These results showed that SVHRP could decrease the AP firing frequency, increase the AP rheobase and threshold, but decrease the AP peak amplitude of hippocampal neurons.

在产生位相放电的45个细胞中，有8个细胞在SVHRP处理后仍可以诱发出位相放电(占17.78﹪)；37个细胞在SVHRP处理后无法诱导出位相放电(占82.22﹪)，SVHRP处理后动作电位的产生与处理前相比，有显著差异(P＜0.01，n=45)；在产生重复放电的7个细胞中，在1×10-2μg/mL SVHRP作用后均不能再次诱发出重复放电，而是产生一个动作电位或不再产生动作电位，药物处理前产生的动作电位个数为14.57±1.00，SVHRP处理后产生动作电位的个数为0.57±0.20，二者之间有显著性差异(P＜0.01，n=7)。1×10-4 μg/mLSVHRP处理后，诱发动作电位产生的基强度由(75.10±8.99)pA增加到(119.85±12.73)pA(P＜0.01，n=8)；阈电位由(-41.17±2.15)mV升至(-32.40±1.48)mV(P＜0.01，n=8)；动作电位峰值由(68.49±2.33)mV下降至(54.71±0.81)mV(P＜0.01，n=8)。

Objectives:Through the detection of the expression level of interleukins-1β(IL-1β), matrix metalloproteinase 9(MMP-9) and nuclear factor kappa B in Synovium and the detection of the concentration of IL-6 and TNF-αin serum of collagen induced arthritis Wister rat, to investigate the relationship between IL-1β、MMP-9、NF-κB and arthritis index in the development of arthritis,and to understand the pathology of synoviocyte.This may provide an experimented proof for the diagnosis of clinical rheumatical arthritis. Methods: Thirty male Wistar rats were randomly divided into two groups,namely the model controlled group and the normal controlled group,three subgroups were randomly divided in model group and controlled group separately.

目的：通过对白细胞介素1β，基质金属蛋白酶-9，转录因子核因子κB在胶原性关节炎大鼠滑膜细胞表达水平的观察，同时监测同期血清细胞因子IL-6和TNF-α的浓度，旨在探讨白细胞介素1β，基质金属蛋白酶-9，转录因子核因子κB与RA发生发展及其与关节指数之间的相关性，了解炎性病变关节滑膜细胞的病理生理状态，进一步为类风湿关节炎的临床治疗提供实验依据。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应，a波潜伏期缩短o叼。05）；术后2周的暗适应视杆反应，a波潜伏期延长o功刀5）：术后1周的暗适应最大反应，b波潜伏期缩短o功刀5卜＊CS大鼠术后4周的暗适应视杆反应和明适应反应，a波振幅增大o功刀匀；术后4周的暗适应视杆反应，a波潜伏期缩短o＜0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡，导致FERG的a、b波振幅明显降低；视网膜下腔仍存在感光细胞外节盘膜碎屑，外丛状层缺乏感光细胞的突触末端，同时Muler细胞出现反应性胶质纤维增生。

Principle of least squares,digital image segmentation,digital image treatment and digital image identifying were applied into the research of wood science in this research,the wood cell image of annual ring from annual center to wood edge was analyzed,the modified level set method was applied to segmentate cell image precisely for the first time,the identifying of wood cells and hexagon simulation for wood cells were accomplished with active contour model,which had important potential application value in deepening the research of wood properties to a further step,moreover,had an important significance for improving comprehend on wood micro-properties and enlarging the applicable range of wood.

本研究将最小二乘原理、数字图像分割、数字图像处理、数字图像识别应用于木材科学研究，分析了从年轮中心到木材边缘的年轮的木材细胞图像，应用改进的水平集方法准确分割细胞图像，应用活动轮廓模型完成了对木材细胞的识别，并完成了木材细胞六角形仿真，对木材材性的深入研究等领域中有重要的潜在应用价值，对提高木材对微观性能认识，扩大木材的适用范围，具有重要意义。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。