细胞性

It also indicates that the expression of WT1 is regulated strictly in time and space, and the aberrant expression of WT1 may result in retarded differentiation of blastemal cell leading NR or nephroblastoma.

提示在散发性肾母细胞瘤发生中除WT1突变外，WT1表达延迟可能是其中起重要作用的机制之一；WT1基因与肾母细胞瘤的组织分化有关，可能诱导胚基细胞向上皮细胞方向分化。

In addition, a metabolic inhibitor, actinomycin D has no effect on enzymatic activities of caspase-1/ICE-like and caspase-3/CPP-32/like proteases during Fas-mediated CD4〓T cell apoptosis. These results indicate that the appearance of caspase-1/ICE-like and caspase-3/CPP32-1ike proteases activities is a post-translational activation of these proteases. It is likely these caspases constituently express in a inactive form in the cytosol of CD4〓T lymphocytes. Once the rest SEB-responsive CD4〓T cells are restimulted, those inactive caspases such as caspase-1/ICE-like and caspase-3/CPP32-1ike proteases are sequentially cleaved to become active forms, and the apoptotic signal is transducted to downstream signaling molecules, Finally leads to CD4〓T cells apoptosis.

另外，通过代谢抑制剂放线菌素D对超抗原SEB诱导静息SEB应答〓细胞凋亡过程中Caspase成员酶活性变化的研究表明Caspase-1／ICE样，Caspase-3／CPP32样胱氨蛋白酶并非新合成的信号分子，很可能以无活性方式组成性表达于静息SEB应答〓细胞胞浆，在超抗原SEB再次刺激诱导〓细胞凋亡早期，先后顺序活化，借以传递凋亡信号到下游信号分子，诱导〓细胞凋亡。

Subacut myeloid leukemia can be a defined type of leukemia. The common characteristics of subacute myeloid leukemia are as follows: the onset is insidious, and anemia and hemorrhage of skin and mucosa are the main clinical manifestations. Hepatosplenomegaly is rare. There are multilineage abnormalities in blood cell counts and increased blasts in the bone marrow with cytogenetical abnormal clone in most cases. The disease course is subacute and progressive.

1、亚急性髓性白血病是一个可以确定的白血病类型，其特征为：起病隐袭，以贫血或皮肤粘膜出血为主要症状，肝脾肿大的特征较少，血象为一系、二系或三系的异常，骨髓细胞形态学检查原早幼粒细胞异常增高，细胞遗传学检查多数有克隆性异常核型，病程中可有克隆演进，呈现亚急性而进行性的临床经过。

Its functions can be divided into three subsets: T helper cells, inhibition of cytotoxic T cells and T cells.

按其功能可分为三个亚群：辅助性T细胞、抑制性T细胞和细胞毒性T细胞。

Staining and observing shows that the most pathological changes were interstitial pneumonia and hemorrhagic pneumonia, typical nonsuppurative encephalitis, and neurogangliitis et al. The lymphoid tissue was necrosis, and the amount of lymphocytes was reduced. The pathological changes of the positive cells, especially the epithelial cells were swell, denaturization and hyperplasy to the extent.

H.E。染色观察显示，病理组织学变化主要表现为间质性肺炎和出血性肺炎、典型的非化脓性脑炎、神经节炎等；淋巴结、扁桃体和脾脏等组织内淋巴细胞坏死、数目减少；有阳性反应的细胞，特别是阳性上皮细胞呈现不同程度的肿胀、变性、增生等病理变化。

OBJECTIVE: To study the culture method of tolerogenic dendritic cells in vitro, and observe the basic characters.

目的：探讨耐受性树突状细胞的体外培养方法，观察不同培养条件下耐受性树突状细胞的基本特征。

Methods Epithelial cells and fibroblast from ameloblastoma, keratocyst, dentigerous cyst, radicular cyst and oral mucosa were cultured and observed by phase-contrast microscope.

原代体外培养成釉细胞瘤细胞、牙源性角化囊肿、根端囊肿及口腔粘膜的上皮细胞和成纤维细胞，倒置光相差显微镜观察。

By the cooperation of 8-MOP/UVA, the photoaging-characteristic biological markers of the dermic fibroblasts in vivo and in intro were changed as follows:① with a permanent switch of mitotic to stably postmitotic phentypes, fibroblasts displayed growth suppression and morphological changes of cell senescence;② increasing expression of SA-β-galactosidase;③ increasing expression of p16 protein;④ continuous up-regulation of mRNA of MMP-1 and MMP-3, while the protein of TIMP-1 was only slightly induced;⑤ high levels of the 4977bp deleted mtDNA accumulated in dermis and cultured fibroblasts, and large accumulation of a A→C base transversion of 414 position of 〓 of human mtDNA control region for replication of cultured fibroblasts also.

2.8-MOP/UVA作用下，体内外真皮成纤维细胞生物学特性出现具有光老化特征性的改变：①细胞由具有分裂活性的分裂表型转化为不具有分裂能力的分裂后表型，形态学出现细胞衰老的相应改变；②SA-β-Gal表达增加；③p16蛋白表达增加；④MMP-1、MMP-3 mRNA持续表达而TIMP蛋白表达仅被轻微诱导；⑤mtDNA 4977bp缺失大量累积，培养成纤维细胞mtDNA复制控制区〓片段414碱基迅速出现大量A→C的点突变。

The main pathological findings include a desquamative interstitial pneumonia-like reaction with intra-alveolar macrophages and numerous large multinucleated histiocytes that ingested inflammatory cells were admixed with macrophages.

GIP主要病理表现有脱屑性间质性肺炎样反应，即在肺泡腔内有巨噬细胞和大量的多核巨细胞聚集，位于肺泡腔内的多核巨细胞内可见被吞噬的炎性细胞，是GIP的特点。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。