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24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组无显著性差异(p>0.05)。本实验结果发现,与单纯缺血12小时、24小时大鼠相比,药物干预组缺血侧大脑皮层TGF-β1免疫反应明显增强,计算机辅助图像半定量分析两组间缺血侧大脑皮层TGF-β1免疫阳性细胞数差异非常显著。说明药物对局灶性脑缺血损伤可能有一定保护作用,其机制可能与诱导脑细胞TGF-β1合成增加有关。然而,药物调控TGF-β1表达,其促TGF-β1表达增强的机制可能是这样的:脑缺血时TGF-β1mRNA表达增加可能与缺氧诱导神经和胶质细胞刺激生长因子表达有关。

According to 5'flank regions and partial sequences of the MyoG gene in Sus scrofa, Bos taurus, Homo sapiens, and Mus musculus published in GenBank, the homogeneous primers were designed to amplify the promoter region of Gansu wapiti MyoG by Touch-Down PCR.

MyoD和MyoG是一类肌调节蛋白,其中MyoD代表肌源性的发生;MyoG诱导骨骼肌的最终分化,调控中胚层细胞分化为成肌细胞,再由成肌细胞融合为肌纤维这一过程,在肌细胞分化过程中起着中心调节作用,是唯一的不可替代的成肌调节因子[2]。

A portion of ductal cells and individual serous acinic cells of the minor salivary gland also expressed Fas not only in membranous but also in cytoplasmic pattern.

结果发现,在正常鼻咽粘膜表面及隐窝的假复层纤毛柱状上皮细胞胞膜、部分小涎腺的导管上皮和个别浆液性腺泡上皮细胞的胞膜和胞浆Fas阳性表达;鳞状化生上皮的表层细胞胞膜有Fas表达,但基底细胞未见Fas表达;浸润的淋巴细胞也不表达Fas.45.9%(17/37)的鼻咽非角化性癌Fas阳性,但只有不足10%的癌细胞胞膜微弱表达Fas。

ConclusionDifferential diagnosis includes adenosis of vagina,metastatic clear cell adenocarcinoma of the endometrium,metastatic clear cell adenocarcinoma of kidney.

应与阴道腺病、转移性子宫内膜透明细胞癌及转移性肾透明细胞癌等鉴别。

The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells, and mainly by antiacid bacteria fibrinous hyperplastic stage is featured by a few fibrous tissue, cells and mucous oweing to hardness to get puncture, neans tuberculous restoration,and scar formation.

结核初期-炎性增殖期60例,占5.5%;结核早期-淋巴结节期130例,占11.9%;结核中期-结核性结节期有590例,占54.1%;结核晚期-干酪样脓样坏死期有280例,占25.7%;结核恢复期-纤维素增殖期30例,占2.8%。炎性增殖反应期非特异性形态学变化,需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断,结核结节期主要可有较多类上皮样细胞及郎罕氏细胞;而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞,此期主要能查到抗酸菌为特点;纤维增殖期,抽出物难取,仅见少数纤维组织、纤维细胞和黏液间质为其特征,提示结核恢复、瘢痕形成所致。

The biological activities of ScFv obtained from 293T cell culture fluids infected by liposome were evaluated. RESULTS: The accession number of VH and VL genes of antiricin neutralizing monoclonal antibody was DQ389248 and DQ389245. ScFv could bind with ricin specifically. Inhibition percentage of culture liquid of 293T cell without dilution and with 2 times dilution was 33.7% and 29%, respectively. ScFv could neutralize the cytotoxic effects of ricin to SP2/0 cells at the concentration of 0.01-0.005 ng/mL in a nice doseeffect relation.

结果: 中和性单抗杂交瘤细胞4C13轻、重链可变区基因在GenBank中的登录号为DQ389248和DQ389245,ScFv与ricin可特异性结合,培养上清原液和2倍稀释液的抑制率分别为33.7%和29%;ScFv在ricin浓度为0.01~0.005 ng/mL时可中和ricin 对SP2/0细胞的细胞毒,随着ricin浓度逐渐增大,细胞存活率逐渐减低。

The rates of apoptotic granulosa cells in all kinds of follicles in immature gilts were ashigh as atretic follicles;it decreased significantly after injection of PMSG,but it was higherand lasted shorter than that of mature pig.

性未成熟仔母猪卵巢中各类卵泡颗粒细胞凋亡比例很高,接近闭锁卵泡的凋亡比例;注射PMSG后则各类卵泡颗粒细胞凋亡比例显著下降,但高于性成熟母猪注射PMSG后的凋亡比例,并且维持的时间也较短。

Results The tissue of 3 cases of ACC in the esophagus was mainly composed of square shape gland epithelial cell and variant myoepithelial cell, mesh shape cavity crack and the tubular, the entity shape arrangement, the cavity crack content basophilia and acidophilia the secretion. There was transmigration of superficial epidermis in 1 case of ACC; All 3 cases were positive of CK, H-CK, P63, S-100 (endochylema /nucleus), EMA, and Bcl-2. Case 3 was positive of CerbB-2 and P53. Case 2 was CD117, AAT, SMA, PAS/AB.

结果 3例食管腺样囊性癌的癌组织主要由立方形腺上皮细胞和变异的肌上皮细胞组成,呈筛孔状腔隙、管状、实体状排列,腔隙内含嗜碱性和嗜伊红分泌物;3例食管腺样囊性癌中例1表面上皮有移行;免疫组化:3例CK、H-CK、P63、S-100、EMA、Bcl-2均,例3 CerBb-2,P53,例2 CD117,AAT、SMA,PAS/AB。

Results The positive rate of binucleated granulocytes was 0%in normal contrast group. It was 53.0%in acute myeloid leukemia, 46.2%in chronic myeloid leukemia, 41.7%in reactive granulocytosis, and 38.4%in myelodysplastic syndrome. they were significantly higher than 8.2%in common anemia, 12.5%in idiopathic thrombocytopenic purpura, and 0%in lymphocytic leukemia.

结果 双核粒细胞阳性率,27例正常对照组为0,急性髓细胞白血病53.0%(80/151)、慢性髓细胞白血病46.2%(18/39)、反应性粒细胞增多症41.7%(20/48)、骨髓增生异常综合征38.4%(33/86),明显高于一般贫血8.2%(8/98)、特发性血小板减少症12.5%(4/32)和急性淋巴细胞白血病为0。

Carotene and bixin inhibited the of MCF-7 human breast cancer cell significantly. The effects of carotenoids were pleiotropic.β-Carotene can activate PPARγ signal pathway and regulate expression of the downstream and relative genes, and impel cytochrome C liberation from mitochndria and increase the level of reactive oxygen species in cell.

胡萝卜素与胭脂树橙显著抑制乳腺癌MCF-7细胞的生长,β-胡萝卜素的抑制作用可能是多效性的,一是激活PPARγ信号系统,调节PPARγ下游基因及相关基因表达,另一个是促使线粒体中的细胞色素C释放,引起细胞内活性氧生成增加,导致细胞活力下降或坏死。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

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