细胞性

To date, most research on DTCNNs has restricted to the stability of a system[17-19,37,38].Few papers consider the existence and attractability of periodic so-lutions.

迄今为止，大多数文献局限于研究离散细胞神经网络模型的稳定性，很少考虑离散时间细胞神经网络周期解的存在性及吸引性问题。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞；表皮细胞周期变化为伤后3天细胞增殖活跃，伤后7天细胞分裂明显增多；伤后1，3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强，伤后3，5天EGF、TGFβ-R2基因表达增强，伤后5，7天TGFβ-R1(转化生长因子β受体1)基因表达增强，伤后7，10天TGFβ1基因表达增强。

The proliferative capacity of LEC from human had directly relevance to the age of donor(r=-0.996).Conclusions The formation of lens-like spherules is characteristics of LEC lines in the cultured cells.Under identical conditions,the proliferative rate of LEC from bovine and rabbit is fast than that of LEC from human,but dedifferentiation of LEC from bovine and rabbit is easier than that of LEC from human;LEC from the three species exhibit similar limited growth potential.The proliferative rate of LEC from human has a inversely proportion with age.

&晶状体小体&的形成可作为确定晶状体上皮细胞株的一项特征性依据，而体外培养的人、牛、兔晶状体上皮细胞具有相同的有限生长潜能，在相同的条件下，牛、兔晶状体上皮细胞的生长增殖速度比人晶状体上皮细胞快，但易于发生去分化；此外，人晶状体上皮细胞的生长增殖率与年龄密切相关，年龄越小，晶状体上皮细胞的生长增殖速度越快。

Different cells,cell density and cell shape lead to comparatively great difference in wood properties and physical mechanical properties, change of wood cells affects change of wood properties including wood quality,wood property and all other aspects,and wood properties decide economic value and directly affect manufacturing and utilization of wood,therefore,the research and analysis on wood cell structure become especially important.

由于树木生长不均匀，使各种树木的木材构造具有多样性，不同细胞体、细胞密度和细胞形状导致了木材材性和物理力学性能存在较大差异，木材细胞的变化影响着木材性质的变化，木材的性质又决定木材经济价值，并直接影响木材的加工和利用，由此对木材细胞结构分析研究就变得尤为重要。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

METHODS The inhibitory effect of nobiletin on proliferation of SMMC-7721 cells was assessed by MTT analysis and growth curve assay. Morphological changes under microscope were observed by Giemsa staining. Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of KM mice. Different dosages of nobiletin 125, 250, 500mgkg^(-1 were injected by intragastric gavage everyday.

不同浓度的川陈皮素作用于人肝癌细胞SMMC-7721细胞，用MTT法、细胞生长曲线实验研究其对SMMC-7721细胞的生长抑制作用，Giemsa染色观察细胞形态变化，初步研究其体外抑瘤作用；建立小鼠H22肝癌移植性实体瘤模型，研究川陈皮素对肝癌的体内抑制作用。

Second, because the thoracic duct drainage can be particularly lymphocytes from the large number of T cells in vitro, to reduce its dermal layer deposition, as well as the release of lymphokines, resulting in T-cell subsets change, helper T cells and the inhibition of T-cells ratio decreased the humoral immune function, B cells to produce specific antibodies decreased.

二是因为胸导管引流术可以把淋巴细胞尤其是T细胞大量排出体外，减少其在真皮层内的沉积，以及淋巴因子的释放，从而导致T细胞亚群的改变，辅助性T细胞与抑制性T细胞比值下降，体液免疫功能改变，B细胞产生特异性抗体能力下降。

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点，符合人类单纯性肥胖病的临床和病理特征；实验研究显示：针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂；针灸能促使白色脂肪细胞直径、面积、体积缩小，减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量，显示：针灸增强了白色脂肪细胞的代谢，促进了白色脂肪细胞向棕色脂肪细胞的转化：肥胖大鼠PVN神经细胞自发放电频率增加，脑内单胺递质紊乱，针灸通过抑制PVN亢奋的自发放电，调整5-HT水平，达到影响食欲，改善代谢的作用；肥胖大鼠血中leptin及Ins水平增高，与正常组大鼠比有明显差异，提示大鼠体内存在leptin及Ins抵抗，针灸后肥胖大鼠随着体重体脂的下降，血中leptin、Ins水平降低，与肥胖组比均有显著性差异，而针灸后大鼠脑内leptin增加，与肥胖组比P.05，提示：针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。