细胞性

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）有较好的抑制生长作用，ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml，对肝癌细胞hepg2抑制作用差， ic50为0.937 9 mg/ml；白花丹石油醚提取部位对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml；乙酸乙酯提取物对肺癌细胞（lu-04）有一定的抑制作用，ic50为0.734 3 mg/ml；水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好，值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

There were the radicle polarity and the plumule polarity in differentiation of the first somatic embryogenesis.

此外，体细胞胚的发生和分裂是不同步的，往往在同一张切片上既可看到单个的胚性母细胞，又可见到二细胞、四细胞以致多细胞原胚并存的多样化原胚细胞群体。

Hen THP-1 macrophages were induced to the foam cells by ox-LDL. Treatment of THP-1 macrophage derived foam cells with apoA-Ⅰ, forskolin (FRK, an adenyl cyclase activator), and SQ-22536 for long periods of time (24 h). In addition, THP-1 macrophage derived foam cells were treated with increasing amounts of FRK (0, 10, 20, 40 and 80 μmol/L) and treated with FRK for increasing time(0, 6, 12 and 24 h).

HP-1巨噬细胞源性泡沫细胞经各种因素处理后，采用油红&O&染色，观察细胞内的脂滴，运用液体闪烁计数器检测细胞内胆固醇流出，高效液相色谱分析细胞内总胆固醇、游离胆固醇和胆固醇酯含量，用逆转录-聚合酶链反应和蛋白质印迹分析法分别检测ABCA1 mRNA与ABCA1蛋白质的水平。

As a functionally and phenotypically distictive T cell subpopulation,CD4+CD25+ regulatory T cells are anergic and retain their ability to suppress antigen-driven response of CD4+CD25- cells in a contact-dependent manner or through a way of secreting immunosuppressive cytokines such as IL-10 and TGF-β.

CD4+CD25+调节性T细胞是一群表型和功能特异的T细胞亚群，具有免疫无能和免疫抑制两大特性，通过细胞直接接触或分泌抑制性细胞因子发挥对CD4+CD25-T细胞的抑制作用。

Results Of 4 cases breast MALT-ML, 2 were CCL type, 1 CCL type with blastoid transformation, monotoid Bcell type. By immunohistochemistry, all cases were monoclonity of B-cell with follicular colonization and lymphoepithelia lesions.

结果：4例乳腺MALT-ML中2例为CCL细胞型，1例为CCL细胞型向母细胞样转化，另1例为单核样B细胞型。4例均显示B细胞单克隆性及滤泡克隆化和淋巴上皮病变。

The relative cell groth rate of L929 cell represented the proliferation of cells on materials with respect to control over time. The result showed that cytotoxicity of thermo-sensitive cell culcure dish was none or little,which can be used for lab use.Additional cell culture was performed with two types of celland cell sheets detachment were observed by reducing the culture tempareture after the confluently growth of the two types of cells.

进一步利用两种不同的细胞永生化的成牙本质细胞系和人牙周膜细胞在接枝有PNIAPm的培养皿上进行培养，观察到细胞可以正常贴壁，生长并增殖，并且在形成连续的细胞层之后，通过降低温度，获得了完整的细胞层结构，结合前两部分实验结果，证实了利用等离子体预处理和紫外线照射接枝制备温敏性培养皿的可行性，为后续研究提供了实验基础。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types .

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types . Followup was achieved in 263 cases (74.93％),with tie 3、5year and 10 year survival rates being 70.45 %(124/ 176),53.3%(40/75) and 23.8%(5/21) respectively.

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。263 例(74.93 %)获得随访，随访时间6个月～12 年，3 年、5 年和10年生存率分别为70。

Pathology findings:there were, 294 cases of clear renal cell carcinoma （83.76 %）, including 2 cases of cystis renal cell carcinoma , 27 cases of Granntdar renal cell carcinoma （ 7.69 %）, 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma, 10 cases of other cell types .

病理结果：透明细胞癌294例（83.76%，其中囊性肾细胞癌2例），颗粒细胞癌27例（7.69%），乳头状肾细胞癌14例（3.98%），肾混合细胞癌6例（1.7%），其它癌10例。263例（74.93%）获得随访，随访时间6个月~12年，3年、5年和10年生存率分别为70.45%（124/176）、53.3%（40/75）和23.8%（5/21）。

It is well known that retinal pigment epithelial cells are predominant proliferative cells in PVR which is an excessively wound healing response occurring after RPE cell wounding. The activated RPE cells migrate from their normal, sessile state to vitreous and onto both surfaces of the retina, in which they begin to dedifferentiate, migrate, proliferate, change phenotype and secrete ECM.

既往研究证明视网膜色素上皮(retinal pigmentepithelial，RPE)细胞是参与PVR的主要细胞，PVR是视网膜脱离后RPE细胞损伤的一种自我修复过程，是一种过度的眼内创伤修复反应；RPE细胞在正常位置上处于静止状态，而在某些病理条件下，如视网膜裂孔形成和视网膜脱离后，RPE细胞脱离原位，开始去分化、移行、增生、发生表型转化并分泌胶原等ECM，最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜，造成牵拉性视网膜脱离。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。