细胞性

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

There were enough CD8+T cells in POF women. But their autoimmune reactions were excessive. It should be advanced studied whether CD4+T cells resist the suppression functions of CD8+T cells, the activity of contrasuppression T cells is excess, which cause the function of CD8+ T cells descent and the functions of CD4+T cells increase relatively.

POF患者体内虽有足量的CD8+T细胞，却自身免疫反应亢进，是否与CD4+T细胞对它们的抑制作用具有抵抗性有关，或是因反抑制性T细胞活性过强，致CD8+T细胞功能下降，CD4+T细胞功能相对增强，尚待进一步研究。

Based on the different permeability of DNA-intercalant dyes YO-PRO-1 and propidium iodide to the membrane of viable, apoptotic and necrotic cells, cell samples were stained with 4μmol/L YP and 4μg/ml PI for 10 min, and the fluorescence intensity of both YP and PI were measured by fluorometer at Ex/Em wavelength of 485/538nm and 530/590nm, respectively.

根据正常细胞、凋亡细胞和坏死细胞的细胞膜对核酸荧光染料的不同选择通透性，用4μmol/LYO-PRO-1和4μg/ml碘化丙啶(Propidiumiodide，PI)染色96孔板中的细胞样品。分别在485/538和530/590的检测波长下借助荧光分光光度计检测细胞样品孔的YP和PI荧光强度。

The results show that the CD29, CD49, CD90 express in the gonium and the primary spermatogenous, but there are not expression in the oocyte, the second spermatogenous and the spermatocyte.

研究结果表明，CD29， CD49， CD90在雌性生殖腺各级卵母细胞中均不表达，位于生殖褶边缘的性原细胞中可见表达；在雄性生殖腺的初级精原细胞中表达，但在成团分布的次级精原细胞、各级精母细胞中均未表达。

Under optical microscope, the cell of NS arrayed as nest, the nuclear was big, deep-stained, thickened-karyotheca, chromatin granulated, Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死，并可见炎细胞浸润；LNT组镜下见少量细胞凋亡，凋亡细胞变小，变圆，核固缩，胞浆浓缩，染色加深，凋亡细胞周围未见炎症反应；LNT+DDP组与LNT组镜下大体相同，但可见到较多量的凋亡细胞。

Each group with drug had effect on inhibiting Lewis lung cancer tumor,LNT DDP had the best effect,46.2%,the rate of inhibitory effect of LNT、DDP was 35.6%、39.7%respectively.3.Under optical microscope,the cell of NS arrayed as nest,the nuclear was big,deep-stained,thickened-karyotheca,chromatin granulated,Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死，并可见炎细胞浸润；LNT组镜下见少量细胞凋亡，凋亡细胞变小，变圆，核固缩，胞浆浓缩，染色加深，凋亡细胞周围未见炎症反应；LNT DDP组与LNT组镜下大体相同，但可见到较多量的凋亡细胞。

Results:A total of 1.4 million cardiocytes can be obtained from one rat and the vital cell was over 90%;Cardiocytes began to adhere and grow after 4～ 6 h,to obviously proliferation after 12～24 h,and to converge together after3～4 days.The cardiocytes showed spherical,fusiform and polygon shape in the visual field of inverted microscope.All cells stretched out parapodium and beated spontaneously and rhythmically.

结果：分离1只乳鼠获得的心肌细胞产量约为140万个，且有活力心肌细胞占90％以上；培养4～6h的乳鼠心肌细胞开始贴壁生长，12～24h明显增殖，3～4 d后细胞融合成片；心肌细胞由圆形变为梭形、星形、多角形，并出现自发性节律性搏动。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

We discovered that the mechanisms of borneol opening BBB involved (1) promoting the tight junction of endothelial cells opening:(2) increasing the quantity of pinocytosis vesicle in endothelial cells and enhance the pinocytosis;(3) inhibiting the activation of P-glycoprotein (P-glycoprotein is an efflux pump of drugs) and increase the permeability of BBB;(4) reducing the expression of intercellular adhesion molecule-1 in brain microvessel endothelial cells;(5) increasing the concentration of Ca〓 in brain microvessel endothelial cells;(6) increasing the activation of eNOS in brain microvessel endothelial cells.

随后，在对冰片开放血脑屏障的机制作进一步的研究时又发现，冰片开放血脑屏障的机制包括以下几个方面：(1)冰片可使血脑屏障内皮细胞间的紧密连接开放；(2)冰片能使内皮细胞内的囊泡数量增加，吞饮功能增强；(3)冰片能抑制P-糖蛋白的活性（P-糖蛋白是一种药物外排泵），而使血脑屏障的通透性增加；(4)冰片能使脑微血管内皮细胞ICAM-1表达量减少；(5)冰片使脑微血管内皮细胞内的Ca〓浓度升高；(6)冰片可升高脑微血管内皮细胞eNOS的活性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。