细胞性

We next demonstrated that OSU-03012 induced reactive oxygen species generation by using H2DCFDA-based flow cytometry and florescence microscopy detection.

大量的ROS可以通过caspase的活化导致凋亡性细胞死亡，但是在某些特定的生理环境下，ROS亦可以在不同的肿瘤细胞中导致自噬性细胞死亡。

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示，该药物具有负性肌力和负性频率作用，降低左室压及其微分，终使每分心输出量减少；缩短豚鼠心室乳头肌动作电位时程的〓和APD〓，大剂量使动作电位平台消失，〓延长；同位素示踪法研究证实L-THP抑制〓摄取，促进〓释放，降低细胞内的〓含量；单通道水平的研究证实，L-THP减少豚鼠心室肌单细胞内向钙电流，且具有剂量，电压，频率依赖性，其作用易于洗脱，药物剂量与Ica也具有高度相关性（相关系数r为-0.87）；L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加，增加谷胱甘肽过氧化物酶的量，使组织细胞免受损伤。L-THP阻断L-型〓通道，抑制〓的摄取，促进〓的释放，降低细胞内〓含量L-THP作用的离子基础。

Candida colonisation did not enhance oalbumin permeation in mast cell deficient W/W mice but did in congenic littermate control +/+ mice. Reconstitution of mast cells in W/W mice by transplantation of bone marrow deried mast cells restored the ability to increase oalbumin permeation in response to Candida colonisation.

念珠菌定植不加强肥大细胞缺陷W/W小鼠的蛋白通透性，而其同系同窝对照+/+小鼠则有加强，W/W小鼠通过骨髓肥大细胞移植重建肥大细胞功能可恢复念珠菌定植后对卵白蛋白通透性的增强作用。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示：支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除，胶原纤维得到了松散，并维持其原有的天然三维网络多孔结构；该材料透水汽性处于3000g.m-2.d-1左右，适合创面恢复；体外降解时间处于25～50h之间，并可根据需要调整工艺条件控制降解时间；拉伸强度介于10.20～11.50MPa之间，具有良好的拉伸强度；收缩温度介于70～85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点，并且其透气性和拉伸强度高、降解性优良且可控，符合组织工程支架材料的要求。

As an ideal prime immunization vaccine,the DNA vaccine has the advantages of high purity,continuous expression of antigens with low dose, repeatable immunization and inducement of memory CTL with high titer.Similarly, recombinant virus can not only highly express the target antigen,simulate the inducement process of virus infection on body immunity,and induce various cytokines,but also be beneficial to the interaction of high-affinity antibodies with T cell receptors,and the generation of Th1 reaction and CTL reaction.

DNA疫苗抗原纯度高，持续表达低剂量的抗原，可多次重复免疫以及诱导高效价记忆性细胞毒性T淋巴细胞反应，是理想的用于初次免疫的疫苗；重组病毒能高效表达目的抗原，模拟病毒感染诱导机体免疫的天然过程，能诱发多种细胞因子，而且有利于高亲和性抗体和T细胞受体的选择，可以有效地产生Th1型和CTL反应。

Factor of 1 nerve nutrition and lack gene of nutrition of ill nerve of blood-vessel of head of courage and uprightness is a group of special protein and peptide kind element, this are familial include nerve to grow factor (factor of nutrition of nerve of sex of NGF), head source , nerve - 3, alkalescent grow into fiber cell factor and nerve of sex of colloid cell source.

现将几种重要的非炎性细胞因子和缺血性脑血管病关系的探究进展作以下综述。1神经营养因子和缺血性脑血管病神经营养因子是一组非凡的蛋白质和肽类分子，该家族包括神经生长因子、脑源性神经营养因子、神经营养素-3、碱性成纤维细胞生长因子和胶质细胞源性神经营养因子等。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比，有学者提出了一种新的以细胞层为基础的组织工程技术，这种技术作为一种新的选择，利用温敏性培养皿，经简单的温度变化，可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质，从而避免了使用蛋白水解酶。

Inflammatory liver injury during chronic HBV infection is probably not the result of increased numbers of infiltrating CD8+ T-lymphocytes, but of Fas-L expression by Kupffer cells and increased cytolytic activity of cells in portal areas.

在慢性乙型肝炎感染期间炎症性的肝脏损伤可能不是浸润性CD8T淋巴细胞数量增加的结果，但可能是由于枯否氏细胞和门静脉区域细胞的溶细胞活力的增加而引起的Fas-L的表达的结果。

However, another investigation12 using the immunolocalization of MMP1, MMP2, MMP9, and MMP14 (MMP14 is a membrane-bound MMP) showed elevated levels of MMP2 and MMP14 in macrophages, as well as in the type II cells and fibroblasts, in emphysematous, compared to nonemphysematous, tissue.

但是在另一项对MMP 1，MMP 2和MMP 14（MMP 14 为有膜MMP）的免疫定位的研究中发现，气肿性组织较非气肿性组织而言，巨噬细胞中的MMP 2与MMP 14水平有升高，II型细胞和层纤维细胞亦如此现象。

In the presence of 500nM TTX, 70%M/T cells presented spontaneous activity. 80%M/T cells with spontaneous activity appeared excitory postsynaptic currents , which could be reverted at 20mv, 14%M/T cells appeared inhibitory postsy-naptic currents , which the reverse potential was -90mv. Few presented both EPSCs and IPSCs.

当细胞外液有1uM的河豚毒，70%的M/T细胞有自发活动，其中80%自发活动是兴奋性突触后电流、它的反转电位是20mv.14%的自发活动是抑制性突触后电流，它的反转电位是-90mv，极少数细胞同时有EPSPs和IPSPs。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新