细胞性

Objective To observe the cellular mechanism of via skin infection of Microphage tropic HIV and further differentiate the infection of M-tropic and T-tropic HIV.

目的 观察巨噬细胞亲和性HIV经皮肤感染的细胞学机制，区别巨噬细胞亲和性及T细胞亲和性HIV感染的差异。

HA increased level of NO by activating NOS after combining with H〓 and H〓 receptors; and on the one hand 5-HT caused brain microvessel endothelial cells constracting strongly, made the tight joint opening, increased the permeability of BBB, and on the other hand 5-HT increased the quantity of pinocytosis vesicle in endothelial cells and enhance the permeability of BBB after comining with 5-HT〓 receptor.

HA与H〓和H〓受体结合，激活NO合成酶，使NO含量增多而诱发BBB通透性增加；5-HT与5-HT〓受体结合，一方面引起脑微血管内皮细胞强烈收缩，使内皮细胞间的紧密连接开放，BBB通透性增高，另一方面可引起内皮细胞转运小泡增加，BBB通透性增高。

AFGF shows greatpotential for clinical applications for therapy of a variety of diseases, such as Parkinson'sdisease, spinal cord contusion injury, neural regeneration in reimplantation of broken-offfinger, brain ischemia, renal ischemia, myocardial infarction, occlusive vascularitis, retinalischemia, gastric ulcer and nonhealing wound and so on. We expressed human acid fibroblast growth factor gene transiently in tobacco byAgro-bacterium mediated vacuum infiltration and studied the factors, which influenced theexpression level.

人类酸性成纤维细胞生长因子(aFGF，FGF-1)是成纤维细胞生长因子超家族成员，来自于三个胚层的多种细胞都可以表达aFGF.aFGF在治疗帕金森综合症、急性脊柱扭曲性损伤、断指中神经功能重建、脑缺血、肾缺血、心肌梗塞、闭塞性脉管炎、视网膜缺血、胃溃疡及难愈合性伤口等多种临床应用方面具有巨大潜力。

Results The diagnostic correspondent rate of obstructive and nonobstructive azoospermia by seminal spermatogenic cells examination was 85%, 95.1% respectively. The spermatogenic arrest at spermatid level, spermatocyte level and spermatogonium level or sertoli cells only were 14, 21 and 6 cases respectively.

结果 精液生精细胞学检查对梗阻性和非梗阻性无精症分型诊断的符合率分别为85%、95.1%。41例非梗阻性无精症中，生精阻滞阶段在精子细胞、精母细胞、精原细胞分别为14例2、1例和6例。

After the application of 100μ M linopirdine to OHCs, the peak K+ current was reversibly blocked and the late K+ current was partly reduced. In addition, the decay time constant of the TEA-sensitive K+ current was prolonged in the presence of 100μ M linopirdine. The inward current in OHCs was totally inhibited after the superfusion of 100μ M and 200μ M linopirdine respectively.

结果发现，在正常细胞外液中，单离外毛细胞可记录到四乙基二乙胺敏感的外向性钾电流和静息膜电位附近激活的内向性钾电流（ the K+ current activated at negative potential， IKn）两种钾电流，而单离Deiters细胞中只记录到外向整流性钾电流。

Histopathologic examination demostrated cystic necrosis in 6 patients, mult ilocular cysticrenal cell carcinoma in 3, and unilocular cystic renal cell carcinoma in 3 patients. 11 patients have been followed up.l patient died of the disease at 9 months. 10 patients had no evidence of disease,whose suvival periods ranged 10 months to 10 years with an average of 3.5 years.

其中肾细胞癌囊性坏死型6例，单房囊肿性肾细胞癌3例，多房囊肿性肾细胞癌3例。11例获随访，其中1例以颅骨转移为首发症状者术后9个月死于肿瘤转移；10例存活至今，存活时间10个月—10年，平均3.5年。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果：1、通过不同感染途径的实验证实，滴鼻感染的小鼠主要表现为间质性肺炎，而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现，随着感染Q热立克次体剂量的加大，动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现，在腹腔注射后第2d的脾和肝脏即可发现病原体，主要脏器的病理变化从第2d到第12d逐渐加重，第7d动物的病变最典型，至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统，在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内，呈多形性，多见圆形和杆状，大小不一。

Results There was not specificity of clinical symptoms in liposarcoma of the orbit, the major clinical aspects were intraorbital occupied lesion and local hyperaemia,swell and pain which was similar to an acute inflammation. Four different subtypes of liposarcomas could be observed in orbit.In past literature,myxoid was the most common subtype（14 cases,70%）,secondly subtype was well differentiated（3 case）,round cell and pleomorphic subtype were rare（each 1 case）.This case was well differentiated mixed component of round and pleomorphic lipoblasts.

结果：脂肪肉瘤临床表现为眶内占位和貌似急性炎症的局部红、肿、痛，无特征性的症状；病理分型4型，文献中报道以粘液样型最多见（14例，占70%）；次为分化良好型，3例；圆形细胞为主型及多型性各1例；分型不详2例；本例为分化良好型为主伴有圆形脂母细胞及多型性脂母细胞成分。

Oncosis is a special mode of non-apoptotic cell death,it is characterized by cellswelling,budding,organelle swelling,increased cellmembrane permeability and caryolysis.

胀亡是一种特殊形式的非凋亡性细胞死亡方式，它的特征性表现为细胞肿胀、起泡、细胞器肿胀、细胞膜通透性增加和核溶解。胀亡理论的提出为临床医学的发展提供了良好的机遇和理论指导。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新