细胞巢

Human rG1-autoreactive T cells injected intraperitoneally have homed to left knee where its epitope rAG1 was injected, and they participated in the development of inflammatory arthropathy.

结论人特异性rAG1反应性T细胞在rAG1刺激下可向关节局部归巢而导致关节炎症反应，提示针对rAG1的自身免疫反应可能参与了RA的发病。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域，用限制酶 Bfm 消化 PCR产物，10 %聚丙烯酰胺凝胶电泳检测。

Objective To explore the homing regularity of different doses of bone marrow cell transplantation.

目的探讨不同细胞量骨髓移植的回巢规律，进一步提高移植效果。

The neoplastic cells form large and small nests with clearcut epithelial differentiation.

肿瘤细胞形成大大小小的具有明确上皮分化的巢。

At day 4, embryoid body was found and cells were positive for actin, nestin and keratin.

培养第4天形成拟胚体，肌肉特异性肌动蛋白、巢蛋白、广谱细胞角蛋白均呈阳性表达。

Now we have known that SARS-CoV encoded 11 varietal proteins. They have 4 constitutive proteins: spike protein, membrane protein, envelope protein and nucleocapsid protein. Spike protein trimer form spike and exsertion? virus peplos on the surface. Spike protein is archi-surface antigen component of virus, it has acceptor binding and membrane fusion activity. Spike protein has important effect at tissue tropism, cell-fusion and toxicity. Nucleocapsid protein can bind genome RNA to shape neucleocapsid. Membrane protein and envelope protein are necessary constituent to shape virus peplos. Spike protein is I-type membrane glycoprotein, it mediate virus binding with host cell epimembranal acceptor and to fuse host cell membrane.

SARS-CoV属于巢状病毒目、冠状病毒科，为单正链RNA病毒，基因组全长约29.7kb，已知编码11种蛋白，其中包括4种结构蛋白，分别是刺突蛋白、包膜蛋白、小包膜蛋白和核蛋白或核壳体蛋白。S蛋白三聚体形成突起，伸出包膜表面。S蛋白是病毒的主要表面抗原成分，具有受体结合和膜融合活性，在组织嗜性、细胞融合和毒力等方面起重要作用。N蛋白与基因组RNA结合形成核衣壳。M蛋白和E蛋白是包膜形成所必需的成分。

Results The nestin-expressing cells were widespread in the mesenchyme of the fetal human pancreatic tissues.

结论从胎儿胰腺中分离的巢蛋白阳性细胞具有骨髓间充质干细胞表型特征。

This was the reason of no mature ovum in worker. But worker has a potential ability to turn into reproductives because of its complete differentiation stage and the growth stage in oogenesis.

由于具有完整的卵母细胞分化期和生长期，在巢群需要的情况下受调节可以进一步发育完成卵黄的形成，因此工蚁具有向繁殖蚁转化的能力。

After 48 hours pre-induction, the percentage of nestin was 24%, which was 4.6% in the control group. After 48 hours induction, BMSCs exhibited the typical form of perikaryon with pyknotic cell body and prominence projected like that of neuron.

诱导48 h后，巢蛋白染色呈阳性，并可见大量神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性细胞出现；对照组均呈阴性表达。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。