细胞巢

Under optical microscope, the cell of NS arrayed as nest, the nuclear was big, deep-stained, thickened-karyotheca, chromatin granulated, Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死，并可见炎细胞浸润；LNT组镜下见少量细胞凋亡，凋亡细胞变小，变圆，核固缩，胞浆浓缩，染色加深，凋亡细胞周围未见炎症反应；LNT+DDP组与LNT组镜下大体相同，但可见到较多量的凋亡细胞。

Each group with drug had effect on inhibiting Lewis lung cancer tumor,LNT DDP had the best effect,46.2%,the rate of inhibitory effect of LNT、DDP was 35.6%、39.7%respectively.3.Under optical microscope,the cell of NS arrayed as nest,the nuclear was big,deep-stained,thickened-karyotheca,chromatin granulated,Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死，并可见炎细胞浸润；LNT组镜下见少量细胞凋亡，凋亡细胞变小，变圆，核固缩，胞浆浓缩，染色加深，凋亡细胞周围未见炎症反应；LNT DDP组与LNT组镜下大体相同，但可见到较多量的凋亡细胞。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

The cells at the edge of the basaloid islands tended to show palisading arrangement. Squamous epithelium on the surface of tumor often accompanied with carcinoma in situ or superficial invasive squamous cell carcinoma. Occasionally cancer nets were connected with basement layer of squamous epithelium laying in the surface, and rare squamous cells or glandlike structures were seen in the cancer nests.

但BSC癌细胞分化较低异型性较强，癌巢周围细胞呈栅栏状排列，肿瘤表面鳞状上皮常伴发原位癌或浅表浸润性鳞状细胞癌；偶见癌巢与表面鳞状上皮基底层相连、或偶见癌巢内形成鳞状细胞，或腺管样结构，这些与ACC不同。

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or were of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes, with micropapillae floating freely in spongy spaces and separated by fibrous septa.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙，微乳头缺乏纤维血管轴心，每个微乳头细胞团和周边的纤维组织均存在无细胞的间隙样结构；瘤细胞CerbB-2、CgA和EMA。

However, most reports on these aspects have been limited to the unmanipulated cells. The majar goals of the second part of this study is to thoroughly explore the effect of ex vivo expansion to the homing-related potential of UCB CD34〓 cells.

基于该领域的研究现状和第一部分的实验结果，第二部分在不同的扩增时点，将CD34〓细胞从扩增产物中再次纯化出来，从动态的角度观察了扩增过程中UCB CD34〓细胞归巢相关细胞粘附分子的表达、粘附功能、趋化功能和趋化因子受体CXCR4表达的变化，从而评价细胞因子介导的体外扩增对UCB HSPC体外归巢相关功能的影响。

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好，未出现死亡及恶性心律失常，4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞，提示干细胞成功归巢，结合苏木精-伊红染色，归巢细胞位于心肌梗死灶周边区域，呈单个或小团聚集；而模型对照组心肌切片未见胞核蓝染的细胞。

Results The expression of Foxo3a, Bim, FasL in oocyte nests and primordial follicles were detected in the nuclei and cytoplasms of some oocytes. The patterns of Bim and FasL positive rates were consistent with that of Foxo3a and TUNEL positive oocytes rates, which reached the peak in the ovaries of 2-day-old rats.

结果 免疫组化显示Bim和FasL都在新生大鼠部分卵母细胞巢和原始卵泡的卵母细胞核内和胞浆高表达，且阳性率曲线都在2d达到高峰，随后逐渐降低，与TUNEL和FoXo3a的阳性率变化曲线一致。

Results Basal cell adenocarcinoma of prostate was composed of poorly differentiated solid carcinoma nests with many cellular nuclear mitoses arranged like basal cell carcinoma. Necrosis was in the middle of the carcinoma nest. Local differentiation to squamous cell, transitional cell and gland and structure like adenoid cystic carcinoma were found.

结果：前列腺基底细胞腺癌为分化差的实体癌呈基底细胞癌样排列，核分裂象多，癌巢中央伴坏死，可见局灶性鳞状细胞、移行细胞或腺管分化，也可出现腺样囊性癌样结构。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。