细胞学

In the early 1980s, many researchers revealed the structure of cytoskeleton mainly on kinetosome in the cortex by the employment of protargol staining and ultrastructure method, and thus t.

20世纪80年代初，许多学者应用蛋白银染色方法和超微结构方法，显示了纤毛虫细胞皮层中以纤毛基体作为主成分的骨架结构，探讨了纤毛虫皮层纤毛器的形态、形态发生及其结构形成机理，在国内外原生动物细胞学领域成为主流。

With histological observation on these processes suggests protocorm like bodies of Dendrobium officinale Kimura et Migo can be considered a ture somatic embryos that origin from a single-cell.

对其再生植株的过程进行细胞学观察后认为铁皮石斛的类原球茎是单细胞起源的真正的体细胞胚。

As a special structure in papillary tumors and appearing as microcalcification in the imaging examination, psammoma body has been considered of great significance in the diagnosis of thyroid carcinoma.

砂粒体作为乳头状肿瘤中的特殊病理结构，并在影像学上表现为微小钙化，对于甲状腺癌，特别是甲状腺乳头状癌原发灶及转移灶的病理学、细胞学、以及影像学诊断具有重要的意义。

RESULTS: HE stained cells were fusiform-shaped with pseudopodia, round or oval nucleus. The cytoplasm was blue after stained with the toluidine blue. The immunohistochemical test revealed that there existed positive expression of type Ⅰ collagen in the cells. In the presence of 10% calf serum, IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.

结果：苏木精－伊红染色细胞多为梭形，有伪足伸出，细胞核为圆形或椭圆形；甲苯胺蓝染色，胞浆为深蓝色；免疫细胞学方法检测表明纤维环细胞有Ⅰ型胶原表达；在体积分数为0.1的血清条件下，胰岛素样生长因子Ⅰ能提高细胞的增殖活性，并且在有效浓度范围内呈剂量效应关系。

A preliminary study on cytology of Chinese Pteris.

标题 中国凤尾蕨属细胞学的初步研究。

A preliminary study on cytology of Chinese Pteris.

文章题目中国凤尾蕨属细胞学的初步研究。

A preliminary study on cytology of Chinese Pteris.

Title 中国凤尾蕨属细胞学的初步研究。

Methods Colposcopy was done in 1986 cases with cervical lesions, among which 131 cases with smooth cervix and punctate vessels were selected and biopsy was taken in these vessel areas.

时临床细胞学检查阳性或可疑有宫颈疾病的1986例患者施行阴道镜检查，选取其中宫颈光滑且阴道镜下可见点状血管者131例行阴道镜下点状血管区活检。

The cytologic features of normal squamous epithelial cells can be seen at the center top and bottom, with orange to pale blue plate-like squamous cells that have small pyknotic nuclei.

在涂片的中央上方和底部我们可以观察到正常鳞状上皮细胞的细胞学特征，鳞状细胞呈圆形，胞浆呈橘黄色，小的淡蓝色的固缩核。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。