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In order to achieve a high concentration and high productivity of exopolysaccharide from Auricularia auricula, the effect of pH on the performance of EPS production in submerged fermentation was investigated. It was showed that the maximum yield of EPS (7.54 g/L) was obtained at pH 5.5, but the mycelial growth rate was enhanced at pH 5.0 and the maximum dry cell weight was

为了实现黑木耳深层发酵生产胞外多糖的高产量和高生产强度的统一,在7L发酵罐中研究不同pH对A.auricula分批发酵生产黑木耳多糖的影响。A.auricula细胞生长的最适pH为5.0,而黑木耳胞外多糖合成的最适pH为5.5。

Akt,AMPK and ERK proteins were detected by western blot analysis,and the distribution of GLUT4 in these skeletal muscles was observed by immunofluorescence. Results The expression of GLUT4 on the cell membrane was significantly higher in the electrically stimulated groups than in the unstimulated groups.

用Western Blot法测定骨骼肌中蛋白激酶B(protein kinase B,PKB/Akt)、腺苷酸激活的蛋白激酶、细胞外信号调节激酶的表达和活性变化,用免疫荧光方法观察GLUT4在细胞膜及细胞内膜的分布。

BACKGROUND: It has been found that xenogenic extracellular matrix may cause a strong inflammatory response in humans during clinical application of decellularized porcine heart valve.

摘要背景:在去细胞猪瓣膜(Synergraft瓣膜)的临床应用时发现异种的细胞外基质引发了人体一个强烈的炎症反应。

Further studies are underway to investigate the exact intracellular mechanisms underlying the effects of the two substances. In Chapter 4, we observed the effects of metabolites of mangrove fungus Xylaria sp. from the South China Sea Coast, Xyloketal A~D, on the activity of acetylcholinesterase in vitro, and the present data indicated that AChE activity was remarkably inhibited by Xyloketal A~D in a dose-dependent manner.

另一方面,在论文第3章离体豚鼠回肠收缩实验中,发现323-A和323-B可剂量依赖性地抑制组胺、乙酰胆碱以及氯化钾所引起的GPI收缩,pD2'依次为5.13、4.97、5.36和5.51、5.56、5.62,并且,通过机制分析发现,323-A和323-B可以显著降低在外界无钙离子存在时由细胞内钙储库释放钙所产生的收缩反应,而对由细胞外钙离子内流所引起的收缩无明显影响。

ZHAO Yede*,HE Qinglian.Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081Objective To investigate the expression level and intracellular distribution of calreticulin in hypertrophic scar-derived fibroblastsand normal skin-derived fibroblasts.

增生性瘢痕(Hypertrophic scar, HS)作创伤及外科手术后常见且十分棘手的皮肤组织疾病,瘢痕组织内成纤维细胞异常活化和细胞外间质过度沉积既是HS的重要组织学特征,也是HS发生、发展的物质基础。

In a previous study, a porous acellular bovine pericardium was developed by our group as an extracellular matrix to repair a surgically created myocardia defect in the right ventricle of a rat model.

再先前的实验里,我们以一多孔性的去细胞牛心包膜,当做天然细胞外间质,来修补缺损的老鼠右心室组织。

Results The physically and chemically treated SIS was pure with more hollows on the surface and undamaged collagen fibers. The cartilage cells could grow, adhere to and differentiate on the layer of SIS and enter into the hollows, secrete a great deal of extracellular matrices.

结果 经物理和化学处理的SIS纯度高、孔隙多,胶原纤维未受损;软骨细胞在SIS材料上生长、黏附、增殖,并能长入材料的孔隙内,分泌大量的细胞外基质成分。

β-glucan enhanced the growth and the phagocytosis of the RAW264.7 macrophage cell line, and effected the ERKS MAPK signal transduction.

一定浓度的葡聚糖对小鼠巨噬细胞的增殖和吞噬有明显的促进作用,细胞外信号调节激酶5信号通路参与了这一过程。

What factors control the cerebellar granule cells to migrate from the external germinal layer into the internal granule cell layer during postnatal development?

什么细胞外因子引导小脑颗粒细胞的轴突在小脑皮层的分子层中生长的?

And also the relationship of the expressions of TN and survivin in breast cancer was assessed.

1。随着乳腺组织从正常到癌变的过程中TN在细胞外基质中的表达逐渐降低,而在上皮细胞中的表达则成上升趋势。

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