细胞外的

The species of extracellular ATP can participate the effect of nerve system not only as the neurotransmitter and modulator but also has a trophic action to glial cell and neuron. It participates in many courses, e.g. cell differentiation and apoptosis, the morphologic change of chondriosome, to stimulate the synthesis and release of cytokine and nerve growth factor. It has a great influence to neurological growth and plasticity.

细胞外ATP类物质不仅可以作为神经递质及调质参与神经系统的功能，还可对胶质细胞及神经元产生营养作用，广泛参与细胞分化及凋亡、细胞线粒体形态变化、刺激细胞因子及神经生长因子的合成及释放等过程，对神经系统的发育及可塑性变化产生重要的影响。

The cells are typically platelike and closely packed together except where they are modified for a particular function, as are guard cells.

表皮细胞是很有特色的一个细胞层，除了某些具有特殊功能部分细胞，如保卫细胞外，通常都紧密的排列在一起。

Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞，导致细胞生长的抑制和凋亡罗伯托利，斯特凡Purgato ，苏埃洛卡默琳，拉菲尔Fronza ，梅奥尼博洛尼亚，西蒙娜Alboresi ，莫妮卡弗兰索尼，罗伯托Corradini ，斯特凡诺斯福尔扎，安德烈Faccini ，杰森先生Shohet ， Rosangela Marchelli ，和安德烈Pession儿科，博洛尼亚大学，圣' Orsola -马尔皮基医院，博洛尼亚，意大利；有机部和工业大学化学帕尔马，帕尔马，意大利和3Center的细胞和基因治疗，德州儿童癌症中心， Baylor医学院，得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞，针对一个独特的序列反义DNA链的第2外显子，并与在MYCN 其氨基端为核定位信号肽。

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d，然后分为两组，实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞，对照组2除加入HO9810外，还加入了无关细胞，用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示，菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三种葡聚糖蔗糖酶，其中前两者同时存在于上清液中和细胞表面，而后者只存在于上清液当中；菌株DM1-2可以产生两种葡聚糖蔗糖酶，分子量分别为183kDa和142kDa，前者同时存在于上清液中和细胞表面，而后者仅能存在于细胞表面；菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三种葡聚糖蔗糖酶，前两者属于胞外酶，而后者与细胞相连；与菌株6055相似，菌株L4可以产生两种同时存在于上清液和细胞表面的葡聚糖蔗糖酶，分子量分别为145 kDa和136kDa，而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

The results show that the secretory ducts are schizogenous cavity which are only distributed in the stem and the leaf.The initial of the secretory duct drives from the ground meristem.

结果表明，分泌道为裂生腔隙，只分布于茎和叶中；分泌道原始细胞起源于基本分生组织；分泌道原始细胞团裂隙的扩大靠鞘细胞的插入和上皮细胞自身的切向伸长；成熟的分泌道呈圆形或椭圆形，由一层上皮细胞和其外的1 ～2 层鞘细胞包被。

All these results suppose that COCH gene may play an architectural function in inner ear mesenchymic extracellular matrix, and this architectural stability is critical for the potassium recycling in cochleae and the normal level of endocochlear potential.

COCH基因功能的初步推论，COCH基因可能编码内耳间质组织中一种分泌型糖蛋白，通过与间质组织中其它细胞外基质成分的结合，共同形成稳定的细胞外基质结构框架。

To master fluid mosaic model of cell membrane;peripheral proteins and integral proteins;the type of extracellular matrix;Collagen;glycosaminoglycan and proteoglycan;elastin; Occliding junctions;anchoring junctions;desmosome hemidesmosome、the structure and function of adhesion belt and focal adhesion;cell coat;cadherins;selectin;Ig-superfamily; integrins

掌握生物膜的流动镶嵌模型；膜周边蛋白和膜内在蛋白；细胞外基质的类型；胶原纤维；糖胺聚糖和蛋白聚糖；弹性蛋白；封闭连接；锚定连接；桥粒与半桥粒、粘着带与粘着斑的结构与功能；细胞外被；钙粘素；选择素；免疫球蛋白超家族；整联蛋白

The result using inhibitors of Ca〓 channels supported above observation. When Ca〓 channels in plasma membrane were blocked by LaCl〓, verapamil or nifedipine, and the Ca〓 channels in endomembranes opened naturally, extracellular CaM couldn't induce 〓 increase. When Ca〓 channels in endomembranes were blocked by ruthenium red and the Ca〓 channels in plasma membrane opened naturally, extracellular CaM induced Ca〓 increase in guard cells, but the extent of Ca〓 increase was lower than that of control.

使用质膜Ca〓通道抑制剂LaCl〓、异博定、尼群地平及细胞内Ca〓通道抑制剂钌红的结果支持这种推测：在质膜Ca〓通道被阻断、细胞内Ca〓通道仍正常开放的情况下，保卫细胞〓不能被CaM诱导增加；细胞内Ca〓通道被抑制、质膜Ca〓通道正常开放的条件下，细胞外CaM诱导保卫细胞内〓升高的幅度不能达到不使用Ca〓通道抑制剂的水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。