细胞基质的

Background: The neuron death resulting from traumatic brain injury can be classified into 3 types:(1)neuron death due to primary injury;(2)neuron necrosis due to secondary injury such as hemorrhage, compression, anoxemia and ischemia;(3)neuron apoptosis due to inflammatory factor, cytokine,, excitatory transmitter, Ca~(2+) overload and oxygen radical et al.

引言创伤性脑损伤所致的神经元死亡主要有三种：①原发性损伤所致的神经元直接死亡；②外伤后出血、压迫、缺血缺氧等继发性损伤因素所引起的神经元坏死；③炎性介质、细胞因子、兴奋性递质、Ca~(2+)超载、氧自由基等因素所诱发的神经元凋亡。

Another interesting phenomenon is that when CaLP and p21 are co-transfected, the expression of p21 can stimulate CaLP translocate from cytoplasm to nucleus. By using methods of bioinformatics, point mutation and flow cytometry, we confirmed the importance of Y139 in the nuclear-cytoplasmic trafficking of CaLP.

激光共聚焦实验还发现当p21过表达时能够引起CaLP从细胞质向细胞核的转移，通过生物信息学方法，免疫共沉淀实验和流式细胞分析表明，CaLP的139位酪氨酸残基存在着较高的磷酸化潜力且在CaLP的核质穿梭和蛋白相互作用中起到了重要的作用。

MT may have important role in the recovery of antioxidase activitiesalleviation of lipid peroxidation and elimination of free radicals, and protection from injury of hepatocytes.

MT对肝脏抗氧化酶活力的恢复、脂质过氧化的减轻、自由基的清除及损伤的肝细胞的保护可能有重要作用。

It is the first time that these phenomena were observed: Two nuclei are surrounded by plasma and locate at the central of the female gametophyte and the others nuclei are positioned at the fringe of the female gametophyte; Before the pollen tube enters the female gametophyte. the nuclei in chalazal end begin splitting of plasma and form multinuclear cell.4. The pollens of Gnetumc are spherical or applanate with single aperture. The ornamentation of exine is spine. The basis part of spine is lenience and the top part of spine is tip or obtuse sphere.

首次在买麻藤属植物的雌配子体中观察到2个游离核位于配子体的中央位置，且被一团原生质所包围的，其余的游离核位于边缘的现象以及花粉管进入雌配子体前，合点端的核已经发生胞质分裂形成多核细胞的现象 4、买麻藤的花粉近球形或扁平型，有单萌发孔，外壁表面具小刺状纹饰，小刺基部宽大，末端尖或钝圆。

Our bodies accumulate lipofuscin and oxygen free-radical damage and random DNA mutations and numerous other microcellular problems.

我们的身体不断遭受着褐脂质，氧游基的损害以及偶然的 DNA 突变和其它细胞问题的折磨。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失