细胞基质的

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料，利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体，采用本实验室摸索出的较为有效的纯化粘细菌的方法，在实验器材上用24孔细胞培养板代替传统的平皿，有效地缩短了粘细菌的纯化周期，提高了分离纯化的效率。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The immunohistochemical location of noncollagenous protein, bone sialoprotein , osteopontin and osteocalcin at different stages following periradicular surgery was undertaken. The crystals formation on surfaces of MTA was evaluated when treated in different conditions without cells in vitro and in vivo. MTA was used to manage clinical problems, including perforations in roots or furcations, and pulpless permanent teeth with incomplete root formation. The study included the following three parts

本研究试图通过体内外研究探讨MTA表面牙骨质形成的机理，动物体内动态观察根尖周组织在用MTA倒充填后的组织反应和细胞外基质非胶原蛋白的表达，体外观察MTA在不同的环境下表面的形态及成分变化，和在体内环境下材料表面磷灰石层的形成，同时结合临床病例观察其临床治疗效果。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

With the assistance of the chaperons such as calnexin、calreticulin and tapasin,the molecular heavy chain andβ2 micro globulin of the major histocompatibility complexare assembled together to form the steady complex which is further transported to the surface of the APC through the Golgi body.

在calnexin、calreticulin、tapasin等分子伴侣的协助下，内质网腔内的主要相容性复合体(major histocompatibilitycomplex，MHC)Ⅰ类分子重链、β2微球蛋白以及相应多肽片段聚集成较为稳定的复合物，经高尔基体被进一步转运到APC表面；MHCⅠ类分子与抗原肽的有效结合是特异性细胞免疫应答的重要的环节。

To identify the possible cytokines which may be invovled in this process,we used neutralizing antibodies of DKK-1 and TGF-βto treat AFMSC when cocultured with K562 cells and found that it was DKK-1 not TGF-βthat participated the process.

由于成熟的基质细胞不存在抑制肿瘤增殖的作用，我们推测可能是AFMSC的内在特性决定了这种作用的发挥。

Angiogenesis is regulated by signals derived from receptors for both growth factors and ECM molecules. Recent studies have shown that activation of ERKs is closely involved in the migration or tubular-like formation of endothelial cells and induced by various angiogenic factors.

血管新生可以受到许多来自於生长因子受器及胞外基质受器的讯息所调控，而近年的研究也显示ERK的活化不但会受到许多促血管新生因子的诱导，而且跟内皮细胞的移型及管状形成作用有很大的关系。

And both YH are higher than the corresponded Yobs, these maybe because the YH not susceptible to predatory, death, regeneration and so on.

进水中不同类型的基质会引起污泥内微生物菌群的变化，不同菌群的微生物其细胞产率系数和内源代谢系数等都不同，这可能是造成污泥产率差异的根本原因。

It is concluded that many genes both known and unknown are differentially expressed in human embryonic aorta-gonad-mesonephros-derived stromal cells. Discovery of these genes provides a solid foundation to elucidate the mechanism of haematogenesis in ontogeny.

筛选AGM区基质细胞中差异表达的基因为进一步研究胚胎造血发育的分化调控的分子机理提供了必要的理论基础。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失