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a simple and stable method for isolation and cultivation human adipose-derived adult stromal cells is successfully established , which provides the foundation for future usage of human adas cells in tissue engneering.

我们建立了一种自人体脂肪组织分离、培养人脂肪基质细胞简便稳定的方法,为其应用于组织工程提供了基础。

Objective To observe the adherent effect of fibronectin coating on bone marrow stromal cells in response to hepatocyte growth factor.

目的观察肝细胞生长因子对骨髓基质干细胞与纤维连接蛋白涂层黏附行为的影响。

Calcium alginate is suitable for the growth of BMSCs and is believed to be an optimal bone and cartilaginous engineering carrier.

藻酸钙适合骨髓基质细胞生长,是一种良好的骨及软骨组织工程载体。

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

AIM:The amnion is a kind of natural extracellular matrix, which has low antigenicity and small reject reaction.

目的:羊膜是一种天然的细胞外基质,具有抗原性低、排异反应小特点。

Many literatures focus on preterm prediction study and evaluate every predictor. The predictors include cervical length and the size of some of fetal organs measured by ultrasound; the level of fFN, IGFBP-1, IL investigated in cervicovaginal fluid, serum relaxin, CRP and HbA1c in mother's blood, MMP-8, MCP-1 and ITAC in amniotic fluid which correlated with infection.

早产的预测是研究热点,大量文献分别从超声测量学角度,包括宫颈长度,胎儿某内脏的测量;宫颈阴道分泌物中多项指标,如胎儿纤维结合蛋白、磷酸化及脱磷酸化胰岛素样生长因子连接蛋白-1、白细胞介素的测定;血生化分析,如血清松弛肽、C反应蛋白、糖化血红蛋白含量的变化及羊水化验与感染有关的一些因子,如快速基质金属蛋白酶8、单核细胞趋化蛋白-1、γ干扰素诱导T细胞α趋化因子等,阐述各指标对早产的预测价值。

The ultimate result of chronic HC injury is the accumulation of extracellular matrix with high density type I collagen within the subendothelial space of Disse, culminating in cirrhosis with hepatocellular dysfunction.

慢性HC损伤的最后的结果是在内皮下间隙高密度的1型胶原的细胞外基质堆积,最后为肝细胞的功能障碍、肝硬化。

Construct PLXRN-IL-1Ra and PLXRN-IL-10 vector, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synovial cells were then transplanted by intra-articular injection into the knee joints, assay of joint lavages confirmed that the gene expression was not lost after 14 days of transfer. Knees receiving the hIL-1Ra had significantly reduced cartilage breakdown. Delivery of the hIL-10 was less effective, When both genes were used together, there was a greater inhibition of cartilage breakdown and a considerable reduction of cartilage matrix degradation.

构建PLXRN-IL-1Ra、PLXRN-IL-10逆转录病毒载体,体外感染同种异体原代滑膜细胞,接着将转染了外源基因的滑膜细胞行关节腔注射入创伤性骨关节炎兔膝关节,通过对关节滑液的ELISA分析证明外源基因的表达在基因转移后14天还很稳定,只接受hIL-1Ra基因治疗的膝关节软骨损坏明显减轻,接受hIL-10基因治疗的膝关节治疗也有一定效果,当两种基因同时导入时,有非常明显的抑制软骨破坏和软骨基质降解的作用。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF. 10 days later after the administration of PLGA microspheres, the effects of stimulus to synthesis of type Ⅱ collagen and proteoglycan can be compared with continue administration of free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短;bFGF缓释微球能通过持续释放活性bFGF,在较长时期内促进软骨细胞的分裂增殖;Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示,应用微球10天后,能有效的促进软骨基质成分的合成,其效果与持续应用游离bFGF的效果相当。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短;bFGF缓释微球能通过持续释放活性bFGF,在较长时期内促进软骨细胞的分裂增殖;Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示,应用微球10天后,能有效的促进软骨基质成分的合成,其效果与持续应用游离bFGF的效果相当。

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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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