细胞基质的

The essential events of the process are detachment, intravasation, circulation, arrest, extravasation, angiogenesis and proliferation, which come down to interactions of neoplastic cells with host cells and extracellular matrix -governed by adhesion, migration, invasion, proteaselysis, and the complicated signal transduction.

而所有步骤均可归结为癌细胞与宿主细胞及细胞外基质(Extracellular Matrix，ECM)之间的相互作用——粘附、浸润、移动、蛋白酶分泌以及复杂的信号转导过程，基于细胞信息传递和调控在细胞生命活动中处于主导地位，癌细胞信号转导系统的作用及相关机制对于揭示肿瘤转移的本质，探索新的抗肿瘤转移途径具有十分重要的作用。

When the pollen tube comes into the micropyle and contact one of the synergids, both synergids do not show any sign of degeneration.

在二细胞原胚的顶细胞和基细胞中，均可观察到与合子中形态相似的双亲质体和线粒体。

RESULTS:①Retroviral vector EGFP-pLNCX2 transfected primary chondrocytes of rabbits,and high expression of transfected chondrocytes could be obtained through preliminary screening of G418.After being screened and expressing EGFP,chondrocytes kept normal morphology,with pseudopod adhering to the wall and matrix secreting vigorously.

结果：①重组逆转录病毒载体EGFP-pLNCX2转染原代兔软骨细胞，经G418初步筛选而获得的高表达格局，可见软骨细胞经过筛选和表达绿色荧光蛋白后，保持正常的形态学，细胞伸出伪足贴壁，基质分泌旺盛。

RESULTS: After co-culture, morphology of bone marrow stromal stem cells was changed, showing that flat cells began to retract and became spindle-shaped and some cells displayed a linear arrangement like Schwann cells.

结果：共培养后的骨髓基质干细胞形态发生改变，原来宽大扁平的胞体开始收缩，细胞胞体呈梭形，部分细胞类似施万细胞呈线性排列。

We review the effect of rare earth ions on bone metabolism as the following three aspects:(1)effect of rare earth ions on differentiation and function expression of osteoblasts and osteoclasts;(2)effect of rare earth ions on the osteogenic and adipogenic differentiation of bone marrow stromal cells;(3)effect of rare earth ions on bone mineral phase of animals.

本文回顾了稀土离子对骨代谢影响的研究进展，探讨了其药用和安全性问题，主要从以下三个方面进行综述：(1)稀土离子对成骨细胞细胞和破骨细胞的分化和功能表达的影响；(2)稀土离子对骨髓基质细胞成骨分化和成脂分化的影响；(3)稀土离子对动物骨矿物相的影响。

High-resolution transmission electron microscopy of biosilica in silica cells, bulliform cells and long cells of rice epidermal systems revealed that silica bodies are made up of rods about 2. 0-3. 0 μm in length and 0.2-0.5 μm in width.

借助高分辨电镜研究水稻叶外表皮系统中的SiO〓和细胞壁基质作用时发现在硅细胞、泡状细胞和外表皮长细胞腔中SiO〓是以长 2.0-3.0μm宽0.2-0.5μm的微杆状结构团聚而成。

Considering that various cell lines demonstrate the same kind of chemotactic activity, it can be concluded that this effect is a kind of universal phenomenon.

实验结果发现热休克后的人和小鼠的细胞培养上清对DC和T细胞有明显的趋化作用，可以使DC和T细胞穿过基质内皮细胞迁移到下层趋化小室中，提示上清中可能含有具有趋化活性的物质。

Microscope examination:in group a,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.at 8 weeks,marrow formed in the drilled holes in group b,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.at 4 weeks,the drilled holes were full of trabeculae.at 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果：2周时a组钻孔区出现少许炎症细胞，边缘出现较多成骨细胞并有骨组织形成，至8周时，钻孔区内形成骨髓组织，只在边缘形成骨小梁结构。2周时b组钻孔区有大量的成骨细胞，边缘有较多骨组织形成，4周时钻孔区内充满新生骨小梁结构，8周时钻孔区内骨小梁成熟，小梁有骨髓组织填充。［结论］骨髓基质干细胞对兔股骨头缺血性坏死有良好的修复作用。

To address this question in an unbiased and genome-wide manner, we developed a new method, methylation-specific digital karyotyping, and applied it to epithelial and myoepithelial cells, stromal fibroblasts from normal breast tissue, and in situ and invasive breast carcinomas.

为了能无偏向地并在基因组范围解答这个问题，我们开发了一种新的方法——甲基化特异性数字染色体组型，并将这一方法应用于上皮细胞和肌肉上皮细胞、正常乳腺组织来源的基质成纤维原细胞、原位及侵入性乳腺癌细胞。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。