细胞基质的

Leucocytic differentiation antigen 40 sign-al access participates in regulation of inflammatory reaction accommodation of major cell component (vascular endothelial cell、vascular smooth muscle cell and macrophage), it is playing critical role in AS process, participates ather-osclerotic occurrence and development ,reside in atherosclerotic plaque cell thro-ugh CD40-CD40L interaction, induce activate by itself , express and secrete profit to happen immune response、inflammatory reaction、hemagglutination t-hrombogenic proteo-cytokine.

白细胞分化抗原40 (CD40)信号通路参与了动脉粥样硬化斑块内主要细胞成分(血管内皮细胞、血管平滑肌细胞以及巨噬细胞)炎症反应的调节，其在AS进程中扮演着关键性的角色，参与了动脉粥样硬化的发生和发展；存在于AS斑块内的细胞通过CD40-CD40L相互作用，导致自身活化，表达和分泌了有利于免疫应答发生、炎症反应、血凝和血栓形成的蛋白质细胞因子，如激活在AS斑块中的关键性细胞成分如黏附分子、炎症因子、基质金属蛋白酶等的产生，导致斑块的不稳定和破裂，而最终导致粥样硬化斑块病变的恶化。

BACKGROUND: Bone morphogenic protein 2 is one of the most important growth factors in the healing of bone fracture. The main function is to promote the undifferentiated mesenchymal cells and bone cells to recruitment and differentiate. It is the promoter of bone formation. The BMP-2 gene is an ideal gene that can be used to repair the bone.

背景：骨形成蛋白2是骨基质中最重要骨生长因子，主要生物学作用是促进未分化的间充质细胞和骨系细胞的募集和分化，是骨生成的启动因子，可以作为骨修复基因治疗的理想目的基因。

The ameloblasts are the key cells during odontogenesis, and they have been already cultured in vitro. The amelogenin is the extracellular matrix synthesized and secreted by the ameloblasts, and it plays a crucial role during amelogenesis.

成釉细胞是牙器官形成的关键细胞，国内外已成功离体培养出成釉细胞，成釉细胞合成和分泌的细胞外基质－釉原蛋白在釉质的形成中起着关键作用。

This study is to further investigate the mechanisms of osteoclastic bone resorption induced by IL-1 with established osteoclasts culture and osteoclasts, bone marrow stromal cells co-culture system.

本文利用建立的破骨细胞培养和破骨细胞、骨髓基质细胞共培养体系，在体外进一步研究IL-1诱导破骨细胞性骨吸收的机制，旨在探讨细胞因子在骨质疏松症骨丢失发生中的作用。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞，通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色，比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

When BMSCs proliferated to enough cell number (7×106). 500 000 cells were centrifuged to a pellet and cultured in chondrogenic medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×106个细胞时，将每500 000个细胞离心制成高密度细胞微球，在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、 BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

When BMSCs proliferated to enough cell number (7×10^6). 500 000 cells were centrifuged to a pellet and cultured in chondrogenie medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×10^6个细胞时，将每500000个细胞离心制成高密度细胞微球，在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

Organic matrixs coming from cells play role in nucleation, growth, morphology, polymorphology of inorganic materials. This control of organic matrixs are mainly behaved by the interfaction between organic matrix and inorganic materials .The processing of organic matrix controlling inorganic material is called as molecular recognition. The mechanism of organic-inorganic molecular recognition contains three facts such as electrostatic attraction, lattice geometry matching and stereochemical complimentary.

细胞分泌的有机基质在无机矿物的成核，生长，形貌，多型和结晶学定向等过程中起到了调控作用，基质对矿化的这种调控作用具体表现在有机或生物大分子和无机离子在界面上的相互作用，有机质对无机晶体的成核，生长，晶形及结晶学定向等的控制过程称为分子识别，有机-无机界面分子识别机制主要包括静电作用，晶格几何匹配和立体化学互补三个方面。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究，解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢，不易形成单层的难题，建立了兔角膜上皮细胞原代培养的方法。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。