细胞基质的

The histopathologic observations of corneas after penetrating keratoplasty in dogs: Thestructure of wholly transparent graft was the same as that of host cornea apart from the upperdonor-host junction where there was minimal irregularity of the corneal lamellae. However, thecorneal lamellae at the lower donor-host junction were neat. The graft with its central part beingtransparent only showed poor coaptation with recipient, in which the graft shifted inward and had marked wrinkles and folds in Descemet"s membrane. On the back of Descemet"s membranethere was hyperplastic stromal fibers that thickened the graft and partially adhered to iris.

5犬穿透性角膜移植术后角膜病理组织学观察完全透明的植片与植床结构一致，仅两者对合处上部纤维紊乱，而下方纤维排列整齐；中央透明的植片与植床对合不良，植片向植床方向嵌入，后弹力层皱缩、折叠，植片下方因有来自植床的基质纤维而增厚，部分对合处与虹膜粘连；浑浊的植片有比较完整的上皮层，植片约2／3深度的基质丧失原来纤维整齐排列结构，代以大量成纤维细胞增生。

The application of SH returns what can prevent the antioxidant inside body to eliminate, reduce cellular loss, proof SH is had fight oxidation [3] . 1 · the matrix of week of protective action cell of 3 pairs of cells can make the cell is avoided suffer a cytolysis poison...

SH的应用还可以阻止体内抗氧化剂的排除，减少细胞的损伤，证实SH具有抗氧化功能[3]。1·3对细胞的保护功能细胞周基质可使细胞免受具细胞溶解毒。。。

Therefore, our data show that in cases when clear cell chondrosarcoma and chondroblastoma pose a diagnostic challenge, the presence of type II collagen in the extracellular tumour matrix significantly supports the diagnosis of clear cell chondrosarcoma and aids in distinguishing it from chondroblastoma.

我们的数据表明，在诊断透明细胞软骨肉瘤和成软骨细胞瘤受阻时，细胞外肿瘤基质中II型胶原的存在对于诊断透明细胞软骨肉瘤相当有帮助，并有助于和成软骨细胞瘤间的鉴别。

From the theoretical angle, to analyze and summarize the morbility reason of Senile Osteoporosis, to approach the effect of the flavone of rhizome drynaria on the lipidision of marrow stroma cell, as well as the senescence and apoptosis of osteoblast through the experimental study; from the clinical angle, to observe the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis, to supply scientific theory basis for clinical application.

从理论角度分析总结老年性骨质疏松症的发病原因，并通过实验研究探讨骨碎补总黄酮对骨髓基质细胞脂质化的影响，以及对成骨细胞衰变和凋亡的影响，从临床角度观察其治疗老年性骨质疏松症的效果，为临床应用提供科学理论依据。

Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble flavone of drynaria rhizome with 10^(-4)~10^(-6)mmolL^(-1) had the inhibition on the osteomast; it with 10^(-8)mmolL^(-1) had a promotion on the osteoblast; it with 10^(-10)~10^(-12)mmolL^(-1) had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast.

结果 骨髓基质细胞经过4周诱导可转化为表达colⅠ和ALP的成骨细胞，经过生长曲线分析，二磷酸盐和骨碎补总黄酮10^(-4)~10^(-6)mmolL^(-1)时，对诱导后的成骨细胞有抑制作用；10^(-8)molL^(-1)有促进作用；10^(-10)~10^(-12)mmolL^(-1)不明显，其中10^(-8)mmolL^(-1)作用最强；基因分析后，发现二磷酸盐和骨碎补总黄酮促进成骨细胞表达colⅠ和ALP，且两者合用效果大于单独用药。

Its pathologic mechanism has the close relation to high blood sugar, the excrescent of kidney blood dynamics and the household hereditary, etc. Following the improvement of molecular biology, in recent years, many people discovered that many varieties of cell factors take part in the process of DN and exertive important functions.

近年来随着分子生物学技术的进步，研究发现有多种细胞因子参与DN的发生发展过程，其中以转化生长因子、血小板衍化生长因子为核心因子，其作用涉及到肾小球血流动力学改变、细胞外基质代谢、细胞增殖和细胞肥大等诸多方面。

BACKGROUND: The histocyte and inflammatory cells played an important role in the invasion and metastasis of lung cancer cells. However, cell interaction influenced matrix metalloproteinase.

摘要背景：组织细胞、炎细胞可能在肿瘤细胞的侵袭、转移中发挥着重要作用，而细胞间的相互作用对基质金属蛋白酶有影响。

Both Fas and NO can lead chondrocyte apoptosis respectively and cause articular cartilage destruction. IGF-Ⅰ, TGF-β, bFGF, BMP and other growth factors are polypeptide agents that can influence cell activity by signal convection. They can accelerate chondrocyte proliferation and proteoglycan synthesis, play the local regulation action on formation and plerosis of bone and cartilage tissue by autocrine or paracrine. They have the ability to induce cartilage formation. Some investigations showed that growth factors can influence chondrocyte metabolism, synthesis of specific Ⅱ type collagen and proteoglycan by co-operation and inhibition. 1. 3 Situation of OA therapeutics The therapeutic methods of OA mainly comprised non-drug treatment, drug treatment, operation treatment, tissue and genetic engineering, et al. Drug treatment is the chief method among them.

若其活性发生改变，则将导致关节软骨基质成分的丢失和进行性破坏；软骨细胞凋亡与OA的发病密切相关，Fas与NO可各自独立介导软骨细胞凋亡，造成关节软骨破坏；IGF-Ⅰ、TGF-β、bFGF、BMP等生长因子是一组通过细胞间信号传递影响细胞活动的多肽因子，具有促进细胞生长、增殖与合成等作用，可通过自分泌或旁分泌方式对骨和软骨的形成和修复起局部调节作用，可促进软骨细胞增殖、分化与蛋白多糖的合成，具有较强的诱导软骨形成的能力，研究表明多种生长因子相互促进、相互抑制，以协同或拮抗方式影响软骨细胞代谢，影响软骨细胞特异性Ⅱ型胶原和蛋白多糖的合成分泌。

Confocal microscopy was used to inestigate corneal thickness, epithelial and stromal cellular density, and subbasal plexus morphology.

用共聚焦显微镜来研究角膜的厚度，上皮细胞和间质细胞的密度以及基质浅丛的形态。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。