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It is also one of the main etiological factors of bronchopulmonary dysplasia. Present researches show that extracellular matrix metabolism disturbance participates in pathophysiological process of acute and chronic lung injury.

目前认为,细胞外基质代谢紊乱参与了急、慢性肺损伤的病理生理过程,作为降解ECM成份最重要的基质金属蛋白酶及其组织抑制因子在这个过程中可能发挥了重要的作用。

Based on the detailed analysis of a variety of factors affecting stability of taxol production in Taxus cells, it was discovered that initial cell density and the relevant concentrations of substrates, elicitors and precursors significantly affected stability of taxol production; farther study showed that addition of elicitors at appropriate cellular state was the key to steadily improve taxol yield, especially, the proper cellular state was at the end of lag phase and at the beginning of the stationary phase, which was also proved by the cellular ultrastructure.

对影响红豆杉细胞紫杉醇产量稳定性的各类因素进行详细分析,发现细胞的接种密度以及与之相适应的培养基的基质浓度、诱导子浓度和前体浓度是影响紫杉醇产量稳定的重要因素;进一步研究发现,选择细胞处于合适的状态时加入诱导子是稳定提高紫杉醇产量的关键,细胞合适状态特别是延迟期末和稳定期初最合适,细胞状态的超微结构观察结果也证明了这一点;同时研究发现采用合适的同步化处理获得的细胞初始周期时相加入诱导子与紫杉醇产量稳定密切相关,且以低温同步化处理得到的细胞周期的分裂中期相的细胞加入诱导子不仅大大提高了紫杉醇的产量,而且诱导子加入时间大大缩短,有利于大大提高紫杉醇生产率。

Cluster of differentiation 44(CD44) is a principal cell surface receptor for hyaluronate, a major component of extracellular matrices.

人类白细胞分化抗原作为一种粘附分子,主要与细胞外透明质酸结合,参与关节软骨基质中透明质酸的代谢,因而它与各种骨关节疾病包括大骨节病关系密切。

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的:研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2(MMP2)、环氧合酶-2(COX-2)表达的影响:方法:将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响,采用MTT法观察舒林酸对SKOV3细胞生长的影响,通过划痕试验观察舒林酸对SKOV3细胞运动的影响,利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的:研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2(mmp2)、环氧合酶-2(cox-2)表达的影响:方法:将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响,采用mtt法观察舒林酸对skov3细胞生长的影响,通过划痕试验观察舒林酸对skov3细胞运动的影响,利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

After the expression completed, BMP and TGF-β were distributed in the chondral matrix and granulation tissues. The bFGF was mainly distributed around non-active mesenchymal cells in the granulation tissues.

表达后BMP、TGF-β较均匀分布于软骨基质以及肉芽组织间质中,bFGF 主要分布于肉芽组织中非活跃的间质细胞周围。

Objective: To examine the property and proliferation ability of primary culturing MSC, investgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

目的:了解骨髓基质干细胞原代培养的特性及增殖能力,探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用;为软骨组织工程提供足量、合适的种子细胞。

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子,我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果:①成功获得纯度较高的脂肪基质细胞,成功诱导其脂向分化,诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变:细胞相互融合,成片生长,变为细长纺锤形;PPARγ免疫细胞化学染色呈阳性;油红O染色脂滴被染成橙红色。

During the course of culture in vitro, the biological function of human nasal septal cartilage gradually degrade in accordance with increasing times of passage, chondrocytes after the third generation are not suitable for human cartilage tissue engineering seeds or studying cytobiology.

人鼻中隔软骨细胞在体外培养过程中,随传代次数的增加而功能活性逐渐降低,第3代以后的细胞不适于作为人软骨组织工程种子细胞和用于细胞生物学等方面研究关键词:葡萄糖氨基浆糖类;软骨细胞;细胞外基质;生物材料

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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