细胞基质的

Matrix metalloproteinases, which degrade extracellular matrix and basement membrane, plays an important role in many inflammatory diseases.

基质金属蛋白酶(matrix metalloproteinases，简称MMPs)是可以分解细胞外基质和基底膜的蛋白质水解酶，在许多疾病中扮演重要的角色。

Results By using optical microscope,the neurocytes of hippocampus in experimental group were observed to denaturalize,tumefy,destroy and disappear.And under electron microscope,the karyon was irregular,nucleoles deviated,chondriosome tumefied,crista collapsed,membrane was damaged,substance cavitated.Such changes were more evident with the increased paroxysm of epilepsy,the parameters between control group and operation group had no difference（P＞0.01）.But make a monofactorial variance analysis among cells' number,area,circumference and integral optical density in different groups,it showed that there were significant differences between optional two groups（P.01;at the same time,the more serious injury in hippocampus,the more powerful GFAP immunoreaction was.

结果： 实验组海马区光镜下可见神经细胞变性、肿胀、坏死及神经细胞脱失改变，电镜可见细胞核形态不规则、有切迹、核仁偏位、线粒体肿大、嵴断裂、膜破损和基质空化等改变，随癫痫发作次数增加上述改变越明显，对照组与手术对照组比较，各参数差异无显著性（P＞0.05），将不同组别大鼠细胞数、面积、周长、积分光密度分别作单因素方差分析，不同组别间均差异有显著性（P.01），同时观察到海马区GFAP免疫反应阳性的胶质细胞随海马损伤的加重而GFAP免疫反应增强。

Objective To investigate the correlation of matrix metalloproteinase-2,-9 (MMP-2,MMP-9) in transitional cell carcinoma of bladder with tumor invasion and metastasis.

目的：探讨基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）的血清含量及组织表达与膀胱移行细胞癌侵袭转移的关系。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Then test of film cell growth by high sugar, LPS, high sugar plus LPS was done with the result showing that FANG KIDNEY capsule can restrain the growth of film cell and composition of external base, it can be concluded that it has obvious effect in preventing the hardening of kidney small cell.

为进一步探讨糖肾胶囊的作用机制，还采用国内外经典细胞培养方法，成功地培养出肾小球系膜细胞，在此基础上进行高糖、LPS、及高糖加LPS诱导细胞膜增生，结果发现糖肾胶囊能抑制系膜细胞增生及细胞外基质胶原合成，证明其对防止肾小球硬化有明显作用。

RESULTS: After 3 days of operation, the BMP positive cells were found in the matrix of experimental group I.

将自体成骨样细胞即刻种植在胶原包埋的聚羟基乙酸基质材料上，然后将该复合体或单纯基质材料移植到兔颅骨的一侧全层骨缺损区，作为实验侧或实验侧。

Our results showed that the cultures with 5 days of preculture in liquid suspension produced the highest chondrocyte representative matrix of sulfated glycosaminoglycan compared to those with I or 3 days in suspension.

我们的实验结果显示，先经过5天悬浮培养之细胞相较於1天或3天悬浮培养之细胞，能产生最高量软骨基质之代表成分&具硫酸基之聚葡萄糖胺&。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Electro microscope analysis showed that NO promoted the chloroplast development, as indicated by increased number and size of chloroplast in both the mesophyll cell and bundle sheath cell of the leaves, and well-developed stroma lamellae and grana as well as increased numbers.

电镜观察结果证实，NO促进了玉米叶片叶肉细胞和维管束鞘细胞中叶绿体的发育，叶绿体数量增多且体积增大，基质片层和基粒数量明显增多且结构完好。

Cellular adhesion molecule is a kind of glucoprotein expressed on the membrane surface of various cells,which mediate the conjunction and adhesion of cells and extracellular matrix.Vascular cellular adhesion molecule-1(VCAM-1) and intercellular adhesion molecule-1 (1CAM-1) are members of CAM.

细胞粘附分子(cellular adhesion molecule，CAM)是一类由多种细胞产生的，调节细胞和细胞间以及细胞和基质间相互结合并起粘附作用的膜表面糖蛋白，主要包括血管细胞粘附分子-1(vascular cellular adhesion molecule-1，VCAM-1)和细胞间粘附分子-1(intercellular adhesion molecule-1，ICAM-1)等。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。