细胞基质的

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

Objective To investigate the relationship between quantitative change of fibronectin or Laminin and growth and differentiation of leukemic cells.

目的 探讨细胞外基质纤连蛋白、基膜连接蛋白与白血病细胞生长、分化之间的关系。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示：支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除，胶原纤维得到了松散，并维持其原有的天然三维网络多孔结构；该材料透水汽性处于3000g.m-2.d-1左右，适合创面恢复；体外降解时间处于25～50h之间，并可根据需要调整工艺条件控制降解时间；拉伸强度介于10.20～11.50MPa之间，具有良好的拉伸强度；收缩温度介于70～85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点，并且其透气性和拉伸强度高、降解性优良且可控，符合组织工程支架材料的要求。

Methods: Effect of SKI, a herbal preparation for lowering adverse Qi, despelling turbidness, supplementing Qi and activating circulation, and Benazepril were observed by cultured glomerular mesangial cell technique in vitro.Results: SKI could inhibit the fibronetin, laminin, and type Ⅳ collagen of ECM, and the action was dose-dependent.

肾小球硬化是多种原因引起肾小球损伤后出现的共同转归，是慢性肾功能衰竭的主要病理基矗近年来，随着对肾小球硬化机制认识的不断深入，肾小球系膜细胞、细胞外基质及各种细胞因子在肾功能进行性减退过程中所起的作用倍受关注。

Objective: To observe the change of Leydig cell and extra-cellular matrix of testis in mice during experimental orchiditis.

目的：研究小鼠睾丸间质细胞和睾丸内细胞外基质在实验性睾丸炎期间的变化。

The decreased function of Leydig cells and damage of ECM in testis during experimental orchiditis might be the factors associated with the poor fertile ability.

发生睾丸炎期间，睾丸间质细胞功能下降，睾丸内细胞外基质受损，这可能是导致生育力下降的原因之一。

Under scanning electron microscopy, along with the extension of the culture time, the chondrocytes grew and propagated well on the surface. On 3 weeks, the cells adhered to the surface, and extended the pseudopodia, by which the cells were connected each other. The cells secreted matrix, which deposited around the cells and the surface of meniscus. In some area, the cells formed the structure like cartilage lacuna.

扫描电镜观察，随着培养时间延长，细胞在材料表面生长良好，细胞密度增加。3周时，可见细胞黏附在材料表面，有伪足伸出，细胞间通过伪足相互连接，并可见细胞分泌的大量基质沉积于细胞周围和材料表面，部分区域形成类似于软骨陷窝样结构。

They are all protein which are combined in the endoplasmic reticulum and secreted outside the cell, and assume the function of shield,strain and osmoses.

均是由细胞内质网合成后，分泌到细胞外基质中的蛋白质（4），具有屏障、滤过、和渗透作用。

Vascular endothelial growth factor is the most critical growth factor for angiogenesis, and matrix metalloproteinase-9 (MMP-9) is associated with extracellular matrix degrading and bone resorbing.

血管内皮生长因子(Vascular endothelial growth factor，VEGF)在血管的新生中起重要调节作用，基质金属蛋白酶-9(matrix metalloproteinase-9，MMP-9)可降解细胞外基质，引起骨吸收。

It is possible to preparing neutralizing MAb which target to active region of Stx2 by synthetical B cell epitope peptides. At present, the crystal structure of the compound of Stx2 and its ligand adenines has been resolved, and the active site of Stx2 is definite, which is the seventy-seventh amino acids, Ganimalon, of Stx2 A1 fragment.

Stx2通过B亚基与受体Gb3结合后，进入细胞，在弗林蛋白酶(一种钙敏感的丝氨酸蛋白酶)作用下A亚基被裂解为A1和A2两个片段，A1片断经内质网进入细胞质暴露毒性活性位点―A1片段发挥N-糖苷酶活性，导致真核细胞蛋白质合成的终止，最终导致细胞的死亡。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。