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P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞,P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%,20%,50%,80%,100%)的胶原/透明质酸支架中,在无血清培养液中三维培养。2周后,用SO染色和免疫组织化学染色分析,观察硫酸软骨素和Ⅱ型胶原合成情况。

Objective To develop an OA kneepad and explore its mechanism and efficacy on the prevention and treatment of osteoarthritis of the knee through observing the effects of OA kneepad on cytokines, matrix-dissolution, hyaluronic acid, oxygen free radical metabolism, articular cartilage micro-structural changes, cell proliferation and apoptosis.

目的 研制OA护膝,并通过观察OA护膝对骨性关节炎细胞因子、基质溶解素、透明质酸、氧自由基代谢、关节软骨宏微观结构变化和细胞增殖、凋亡的影响,探讨其防治膝骨性关节炎的机制及疗效。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果:诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

We found that during sexual maturation, a low level of SPARC expression occurred in ovary matrix, and moderate in theca cells, but there was no signal in granulosa cells and oocytes.

在eCG诱导的卵泡发育过程中,注eCG 6小时之后,SPARC在卵泡膜细胞和卵巢基质中的表达开始上调,之后显著增强,48小时在膜细胞和卵巢基质中都强表达。

On the other hand, extract of cemental matrix was separated and clarified from healthy teeth which were extracted because of orthodontic treatment.

从因正畸需拔除的健康牙体表面获得牙骨质基质提取物,分别观察不同作用时间、不同浓度的牙骨质基质提取物对牙龈成纤维细胞和牙周膜成纤维细胞在牙体表面附着的影响。

This investigation include two parts: lThe primary culture, chondroblast induction and evaluation of MSC. 2The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan.

第一部分人骨髓基质干细胞的原代分离、体外培养、成软骨诱导及鉴定目的:了解骨髓基质干细胞原代培养的特性及增殖能力,探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用;为软骨组织工程提供足量、合适的种子细胞。

GRP can also make mice's MNC and hematopoietic stem cells from human's umbilical cord blood amplification. We use colony formation to analysis the linear relation during the cells'growth and the medicine's dosage, examine the amplification of CFU-GM induced by hypothallus and its supernatant, RT-PCR is used again to check three cytokines'genes.

发现GRP对小鼠MNC扩增有作用,从而将GRP运用到人脐带血MNC的扩增分化上,用集落形成法分析药物与扩增的线性关系,基质层、基质上清对CFU-GM的影响,用RT-PCR技术检测基质层细胞生长因子基因的扩增。

Our data suggest that the neurogenic potential of pATSCs is markedly higher than that of pBMSCs.

我们的数据显示脂肪基质细胞的神经分化潜能明显高于骨髓基质细胞的。

The results showed that both sporophores and stromata of Cordyceps militaris had high content of polysaccharide. And polysaccharide content in stromata was 2-4 times higher than that in sporophores. CMPS could decrease the AST, ALT and MDA levels, and restore GSH-Px content and cell viability remarkably in supernatant of cultured hepatocytes injured by CCl4 induction.

结果表明:蛹虫草的子实体和基质都含有丰富的多糖,其中基质中的多糖含量最高,约为子实体的2~4倍;CMPS可明显降低由CCl4诱导的损伤肝细胞培养上清液中谷草转氨酶、谷丙转氨酶的活性水平和丙二醛的含量水平,显著提高由CCl4诱导的损伤肝细胞培养上清液中谷胱甘肽过氧化物酶活性和诱导损伤肝细胞的存活率。

The results demonstrated that laurocapram disrupts the highly ordered lipid structure of stratum comeum and makes the structurs of SC loose,which causes the outermost cell of SC easy to exfoliate and decreases the barrier function of skin for the transdermal absorption of drug;Meawhile, laurocapram penetrating into the intracellular matrix of SC cells fluidizes the filaggrin matrix protein and swells the dermal SC, which could increase the free volume of drug and water content in the SC.

探讨氧酮促进洛美沙星透皮吸收的作用机理。结果表明,氮酮通过以下途径促进药物透皮吸收:(1)作用于表皮角质层细胞间脂质。使角质层变得疏松。细胞间距增大。外角质层细胞易于脱落。降低了皮肤对药物的屏障作用;(2)氮酮进入角质细胞内。与细胞内基质相作用。引起基底角质层肿胀。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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