细胞坏死

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

Day puppies were inoculated by abdominal cavity and nasal cavity with CDV,canine distemper patho-sample of duplicating meet with success and through differentdrug of treatment, specific antibody, dexamethasone, causing death by letting. Theresults of pathohistology observation on 14 PID: Control group, the hearts showedcardiac muscle hemorrhage and necrosis, the livers showed a large area of hepatictissue hyperemia, hemorrhage and necrosis, the spleens showed the quantity oflymphocyte grow downwards, the blood vessels of lung congested, congestion andhaemorrhage were serous fluid exudation, the kidneys showed heavy haemorrhageand hyperemia, the cerebrum showed the number of microglial cells of cerebrumincreased.

通过特异性抗体和地塞米松、特异性抗体、地塞米松三种不同的方法治疗后，放血致死，取材作病理切片，观察各组病理组织学变化，结果为：感染后14d阳性对照组的心脏表现为心肌出血、坏死；肝脏表现为肝组织大面积充血、出血坏死；脾脏表现淋巴细胞数量大量减少；肺脏表现为肺泡结构破坏，有坏死灶；肾脏表现为弥漫性的充血出血；大脑表现为胶质细胞增多。

Expresion of FasL was found mainly in the cytoplasm of liver-infiltrating mononuclear cells,these positive cellswere found particularly at the advancing edges of piecemeal necrosis,bridge necrosis and in the intralobular.

以免疫组化法研究Fas配体在丙型肝炎病人肝组织内的表达情况，发现在碎屑样坏死、桥样坏死及灶性坏死区浸润的单个核细胞有明显的FasL表达，阳性率为41.2％。

Results Cells cultured in serum-free medium AIMV could proliferate,kill target cellsand express CD11 c well enough to substitute the cells cultured in standard serum-containing medium.Proliferation,cytotoxicity and expression of CD11 c of A-NK were better than nonadherent natural killer cells.Death patterns of tumor target cells were necrosis and apoptosis.

结果 与完全培养基培养细胞相比，AIMV培养细胞增殖能力、杀伤肿瘤细胞的能力、表达CD11 c 的能力与之相当；在同一培养条件下A-NK细胞的增殖能力、杀伤活性及表达CD11 c 的能力明显强于NA-NK细胞；被A-NK细胞杀伤的肿瘤细胞死亡形式是溶解坏死和凋亡。

Results Cells cultured in serum-free medium AIMV could proliferate,kill target cellsand express CD11 c well enough to substitute the cells cultured in standard serum-containing medium.Proliferation,cytotoxicity and expression of CD11 c of A-NK were better than nonadherent natural killer cells.Death patterns of tumor target cells were necrosis and apoptosis.

结果 与完全培养基培养细胞相比，AIMV培养细胞增殖能力、杀伤肿瘤细胞的能力、表达CD11 c 的能力与之相当；在同培养条件下A-NK细胞的增殖能力、杀伤活性及表达CD11 c 的能力明显强于NA-NK细胞；被A-NK细胞杀伤的肿瘤细胞死亡形式是溶解坏死和凋亡。

The results indicated that the typical apoptosis was induced by PRRSV in lungs and uteri. The ultrastructural changes showed different characterizations at different stages of the infection. During the early stage from 8th h to day 3 postinfection,the apoptosis cells shrank,the cytoplasm concentrated,the chromatin condensed,the kernel disaggregated and the endoplasmic reticulum dilated. During the middle stage from day 5 to day 9 postinfection,the apoptosis cells evidently shrank,the cell membranes protu berated,the plastosome manifolded,and apoptosis bodies were found. During the last stage from day 10 upwards postinfection,the apoptosis bodies degenerated and disappeared,and no inflammation occurred. Few apoptosis cells were necrotic.

结果表明，PRRSV可诱导宿主肺和子宫发生典型的细胞凋亡，表现为细胞的超微形态结构随着病毒感染进程出现不同的特征性变化，早期（感染后第8 h至第3 d）凋亡细胞多表现为细胞体积缩小，细胞质密度增强，染色质浓缩，核仁解体，内质网扩张；中期（感染后第5 d至第9 d）凋亡细胞体积显著缩小，细胞膜突起，线粒体增多，有凋亡小体形成；晚期（感染后第10 d以后）凋亡细胞形态多表现为凋亡小体降解和消失，少数凋亡细胞在凋亡晚期表现为坏死细胞。

Results:According to their size, 68 cases of intramural and subserous hysteromyomas were exposed to HIFU, which had focusing property.After treatment, the total efficacy was 100%(68 cases), while cure rate and significant efficacy were 34%(23 cases) and 53%(36 cases) respectively.

利用 HIFU在人体内部的可聚焦特性，用瞬间高温致肌瘤细胞发生不可逆的变性和凝固性坏死，坏死的肌瘤细胞逐渐被周围组织吞噬、吸收，达到其&纯无创&治疗子宫肌瘤的目的。

In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis, necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.

第二章，利用电镜技术、DNA末端标记、MTT活力测定等方法，研究了毫米波对红白血病K562细胞株、人类急性早幼粒白血病细胞株HL-60及正常人骨髓单核细胞的细胞超微结构、细胞凋亡、细胞活力的影响，结果发现毫米波辐射对正常人骨髓细胞及白血病细胞的影响明显不同，正常人骨髓细胞所受影响较小，特别是功率密度小的毫米波对其无影响；而白血病细胞受辐射后发生显著的凋亡或坏死，细胞活力显著下降，该效应具有明显的功率窗和时间窗。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均，全层或局部区域显著变薄向外膨出，内皮细胞空泡变性或坏死脱落，部分内皮细胞剥离并突入管腔成游离状，可见血栓形成及部分血栓机化；内弹力板变薄、消失或突然中断；在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原；部分动脉瘤壁呈均质状玻璃样变，平滑肌细胞明显减少；中膜和外膜可见大量的炎性细胞浸润；瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞，胞浆内充满脂类物质及胆固醇结晶；部分动脉瘤腔内充满血栓，有的血拴已经纤维化，血栓基部机化，血栓表面有红细胞和纤维素。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。