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Nucellar cells degenerated in advance under low temperature, which resulted in thickened embryo sac wall surrounding the megasporocyte.

低温处理的蝴蝶兰,胚囊发育过程中珠心细胞提前退化,在大孢子母细胞外围形成较厚的胚囊壁。

These results suggest that it is feasible to produce artificial muskrat scent using cell engineering by isolating, culturing cystglanitd epithelial cell and furthmore establishing a musdrat cystgland epithelial cell line.

提示,通过体外分离培养麝鼠香囊腺上皮细胞,建立香囊腺干细胞系,进而用细胞工程方法大量生产人工麝鼠香是可能的。

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养,并应用免疫细胞化学方法,对培养细胞进行染色标记和观察,系统了解哺乳动物听觉细胞发生、发育过程,以及这些细胞在体外的增殖和分化过程,旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源,发现细胞生长的前体细胞。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞,近交系C57BL/6J为受体囊胚供者,采用囊胚注射法构建嵌合胚,体外恢复培养后移植进动情期子宫中,结果生出了黑白杂合的嵌合鼠。

Results Of the 11 cases, radical nephrectomy was done in eight, and partial in three; multilocular cystic RCC was confirmed in eight cases, unilocular cystic RCC in three. Histologically, clear cell carcinoma in nine cases, and Chromophobe carcinoma in two. All were followed for 3-61 months (27.9). Ten patients survived with tumorfree.

结果 11例病人中8例行肾癌根治术,3例行肾部分切除术;多房囊性肾癌8例,单房囊性肾癌3例;透明细胞癌9例,嫌色细胞癌2例。11例均获随访,随访时间3~61个月,平均27.9个月,无瘤存活10例。

Purpose: 1. Zonular fibers are a specific form of extracellular matrix composed mainly of fibrillin what believed to be secreted by non-pigmented ciliary epithelium. The variation of fibrillin-1 distribution in lens capsule after zonule rupture was investigated in the research.

目的:1。晶状体悬韧带是一种特殊的细胞外基质,主要由睫状体非色素上皮细胞分泌产生的fibrillin-1(微纤维蛋白-1)所组成,本研究的目的是观察悬韧带离断后,晶状体囊膜中fibrillin-1的变化,探讨悬韧带离断对晶状体囊膜组成的影响;2。

Results With the expression in normal salivary gland as a standard, low C-erbB-2 mRNA expression was seen in adenolymphoma, basal cell adenoma. However, various degrees of over-expression of C-erbB-2 oncogene mRNA were detected in pleomorphic adenoma, mucoepidermoid carcinoma,acinic cell carcinoma ,adenoid cystic carcinoma ,papillary cystic carcinoma,and myoethelial cell carcinoma.

结果 正常涎腺和涎腺肿瘤中发现不同程度C-erbB-2mRNA的表达,以正常涎腺组织的表达水平为基准,在腺淋巴瘤、基底细胞腺瘤呈现低表达、无表达或与正常涎腺组织表达水平相似,多形性腺瘤、粘液表皮样癌、腺泡细胞癌、腺样囊性癌、乳头状囊腺癌和肌上皮癌则呈现不同程度的过表达。

Pox formation increased from 13.3%-20% to 33.3%-40% after passaging through 9 days old chicken embryo. Typical virus particles were observed under transmission electronic microscope in ultrathin sections of pathological tissues, allantochorion membrane of chicken embryos and affected cells.

用待检病料感染的BHK-21细胞培养物接种9日龄鸡胚绒毛尿囊膜,随着传代次数的增加,痘斑病变的出现率从13.3%~20%上升至33.3%~40%;用山羊痘病变皮肤、鸡胚绒毛尿囊膜和感染细胞进行超薄切片,在电子显微镜下可以观察到典型的山羊痘病毒粒子。

In fish and amphibia, the transition from maternal gene transcription to zygotic transcription happens during the time of midblastula formation, this stage is named by midblastula transition, at this period, embryo cell cycle composition changes, it is the first time to G1 time appearance, the cell cycle timelimit lengthens and the cell division synchronism loses, the large quantities of zygotic gene transcription starts.

鱼类和两栖类由母型调控向合子型调控过渡发生在中囊胚形成阶段,称为中囊胚过渡(midblastula transition,MBT),胚胎细胞周期时相的组成在此期间发生变化,首次出现G1期,周期时限延长,细胞分裂的同步性丧失,大批合子型基因活化转录也在此时开始。

To understand the degeneration of unfertilized aposporous embryo sac in an aposporous species Pennisetum squamulatum, the central cell in the unfertilized embryo sac was characterized ultrastructurally.

为理解植物无孢子生殖胚囊未受精条件下的退化,对无孢子生殖植物非洲狼尾草未受精成熟胚囊中央细胞退化做了细胞形态学研究。

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