细胞囊

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果：与无上皮细胞相比，共培养明显促进了小鼠桑椹胚率（71.3%和61.2%，p.05），囊胚率（50.0%和39.7%，p.05），囊胚孵化率（34.8%和24.3%，p.05）和胚胎总细胞数（44.1和35.6，p.05）。结论：子宫内膜上皮细胞能够促进小鼠胚胎的体外发育，对早期胚胎的体外发育环境具有优化作用。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上结果显示，6－DMAP能增加胚胎干细胞异种重构卵的卵裂率，对重构卵囊胚的发育率影响不大；高培养代数和低培养代数的胚胎干细胞用于异种核移植不影响异种重构卵的卵裂率和囊胚发育率；用胚胎干细胞作为供核细胞；比体细胞作为供核细胞所构建的异种重构胚更容易进行重编程，并且胚胎于细胞指导异种克隆胚胎正常发育的能力强于体细胞。

A core needle biopsy misdiagnosed PASH in 13 cases out of 28 cases and vacuum-assisted biopsy correctly identified PASH in all 3 cases.

4细胞性纤维腺瘤和囊肉瘤：PASH缺乏真正的间叶细胞过生长的现象，其细胞缺乏多形性，无核分裂象，也没有纤维腺瘤或囊肉瘤的上皮成分的生长类型。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

During light adaptation, the diameter of rhabdoms was small, the arrangement of microvillus in the distal rhabdom was in disorder, and the area of the multivescicular bodies in the cytoplasm of the retinular cells was large, but the perirhabdomal vacuoles was very small, the amount of the lamellar bodies and mitochondrias were small, the pigment granules were distributed in all retinular cell.

结果显示：光适应时感杆束的直径较小，组成近远端感杆束的微纤毛排列零乱；小网膜细胞中的多囊体体积增大，膜下储泡囊体积减小，板膜体、线粒体等细胞器数量较少，色素颗粒分布于整个小网膜细胞。

（1） In common cavity malformation, in addition to the abnormal bony labyrinth such as the cochlea, vestibule and lateral semicircular canal, the inner ear perceptive organ such as the saccule, utricle and scala media were also abnormal.（2） Though we could not identify the basal membrane and sensory epithelia under micro-endoscopy, the utricle and saccule could be clearly identified.（3） During cochlear implantation in common cavity malformation, electrode insertion should be monitored under micro-endoscopy, the electrodes should be put close to the anterior wall of the cavity, otherwise the vestibular organ may be damaged.

（1）共同腔畸形极重度聋患者不仅前庭、外半规管和耳蜗的骨性结构形态发生了变化，而且腔内耳蜗中阶与前庭的球囊、椭圆囊的膜迷路结构形态也发生了变化；（2）微窥镜下分辨不出基底膜或听神经细胞的形态结构，可见畸形前庭的球囊、椭圆囊的囊性结构；（3）进行共同腔畸形人工耳蜗植入手术时，应在耳蜗内镜的监视下，将电极摆放到准确位置，尽可能贴近共同腔的前壁，不应损伤前庭器官。

The juice sac primordium originated from a single cell of the carpel-lary endoepidermis with partial participation of subepidermal cell just belowthe initial cell.

锦橙和温洲蜜柑的汁囊最初是从心皮内表皮的单个细胞发生的，后来原始细胞下面的细胞也发生分裂，部分参与了汁囊原基的形成。

There are two types of maturation according to our observation.One is nucleocapsids obtained its tegument in the nucleus and enveloped from the inner nuclear membrane.Another is nucleocapsids entered cytoplasm through nuclear membrane,then obtained their tegument in the cytoplasm,enveloped from the plasma membrane,finally released by necrosis,exocytosis or other ways.

病毒成熟有两种方式：一为细胞核内核衣壳在核内获得皮层，通过核内膜获得囊膜成为成熟病毒；二为核内核衣壳通过内外核膜进入胞浆，核内和胞浆内的核衣壳在细胞浆中获得皮层，然后在各种质膜上获得囊膜，最后成熟病毒通过细胞破裂或其他方式释放到细胞外。

Hyphae, loaded with CTC under pH 6. 8 condition and then grown in pH 8. 0 medium for a little while to destroy the apical acidification, could gave very diffuse fluorescence images of CTC membrame bound Ca〓 and vermiform fluorescence spots due to mitochondrias under pH 6. 8 no longer were clearly distinquished, which implied that besides mitochondrias, other cellular organelles with Ca〓 also were stained with CTC. Thus mitochondrias are not the only one intracellular Ca〓 storage, endoplasmic reticulums and Golgi bodies in the same zone may be also the Ca〓 storages. But the extreme apical zone under 2μm of the growing hyphal tip was still almost devoid of stain under pH 8. 0.The CTC fluorescence was concentrated in the subapic zone beyond about 2μm from the tip and then gradually became lower behind about 40μm from the tip. These results did not support the hypothesis which suggested that the cell wall vesicles in the extreme apical zone were intracellular Ca〓 storages.

将菌丝在pH6.8条件下负载CTC，再置于pH8.0培养介质中短暂生长一会儿以消散菌丝顶端的酸化区域，则CTC膜结合Ca〓呈现弥散的荧光影像，在pH 6.8培养条件下显示的蠕虫状的线粒体荧光斑点不再能够清晰辨认，说明除了线粒体之外，还有其它含Ca〓细胞器，也被CTC染色，线粒体并不是细胞内唯一Ca〓库，还可能包括内质网、高尔基体等细胞器，但菌丝最顶端2μm以前的细胞壁泡囊区域不能被染色，最大荧光强度仍位于菌丝顶端2μm以后区域，约45μm以后荧光变弱，实验结果不支持细胞壁泡囊为菌丝胞内Ca〓库的假设。

VP2 protein of infectious bursal disease virus was displayed on T7 phage surface, and this recombinant phage was then purified and labeled with FITC. The interaction between fluorescigenic phage and IBDV host cells was detected by fluorescent microscope and flow cytometer.

将鸡传染性法氏囊病病毒衣壳蛋白VP2展示到T7噬菌体表面，以FITC标记纯化的重组噬菌体，通过荧光显微镜观察与流式细胞仪检测，研究标记噬菌体与病毒受体细胞——法氏囊B细胞的相互作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。