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METHODS 1、Rat Tenon's capsule fibroblasts were cultured from tissue plants.

1。采用植块培养法培养大鼠Tenon's囊成纤维细胞,并对其进行形态学及免疫组化鉴定。

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体,以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

Monosomic 3, monosomic 5, monosomic 10, trisomic 4 and trisomic 5 as experiment materials, their meiosis behavior of pollen mother cell, pollen characteristics, physiological and biochemical characteristics of anther, section observation of pollen development and the structure of embryo sac were studied, and the effects on the seed set and transmission rate were analyzed.

本文以大白菜同源四倍体小孢子培养产生的且已鉴定为3号单体、5号单体、10号单体、4号初级三体、5号初级三体的非整倍体株系及二倍体株系等为试材,对各材料的结籽率、雄配子传递率进行了统计,并对花粉母细胞减数分裂行为、花粉特性、花粉发育和胚囊结构以及花药生理生化特性等方面进行了观察研究,进而分析了其对大白菜单体n-1雄配子和三体n+1雄配子传递率的影响。

Vesicle A small vacuole of variable origin and shape. It may contain secretory products, e.g.

泡囊:来源不同、形状各异的小型液泡,其内可含细胞分泌产物。

In the vegetative cell, the membrane of a kind of vesicular displayed ATPase positive reaction.

在包囊游仆虫营养期细胞中,酶反应颗粒沿食物泡膜呈规则的、连续的排布。

Since protein transport is a complicated multistep process, it can be predicted that more Rab proteins exist to regulate the vesicular traffic.

细胞内的囊泡运输是一个重要而又复杂的过程,这就意味着有多种Rab蛋白参与其中的调节。

Membrane remodelling1, 2, 3, 4, 5 plays an important role in cellular tasks such as endocytosis, vesiculation and protein sorting, and in the biogenesis of organelles such as the endoplasmic reticulum or the Golgi apparatus.

生物谷报道:细胞膜远不止是细胞外的一层包裹物,对其拓扑进行的重塑研究,将它们与内吞作用、囊泡形成和蛋白分选等重要功能联系了起来。

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上,用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻,胚胎解冻后,囊胚发育率分别为:90%、90.1%、89.2%、91.4%;移植后着床率分别:50.0%、47.1%、43.4%、46.5%,差异不显著;并且与对照组相比差异也不显著。

Comparison of genes in yolk sac cells with a total of 1 200 genes in the control cells, 79 genes differently expressed between the two groups were detected.

在神经管缺陷大鼠胚胎卵黄囊细胞和对照组1 200个基因中,共筛选出表达差异基因79个,其中42个基因表达上调、37个基因表达下调。

The gonads form from a group of large granulated yolk sac cells which invacle the germinal ridges.

性腺是由一群大的颗粒的卵黄囊细胞入缀生殖峭而形成。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

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其优点在于可以在较小的单位中应用。