细胞囊

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内，隐孢子虫出现连续发育阶段，包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊，在60～72 h内形成卵囊；用感染48 h的细胞培养上清接种于免疫抑制小鼠， 10 d后有隐孢子虫卵囊排出。

This paper reviewed the research progresses about the cysts, especially the resting cysts of protozoans, mainly focused on the characteristics of morphologies, structures, constituents, and living activities, as well as molecular mechanism and significance of encystment for the protozoans, also, cell behavior during encystment and excystment was involved based on the references reported.

以国内外文献报道为依据，综述了原生动物包囊，特别是休眠包囊的研究进展情况，主要集中在包囊的形态、结构、组成、生命活动特征、包囊形成的分子作用机理和意义，以及包囊形成与解脱过程中细胞行为上的研究等方面。

Toreniafournieri. has a naked embryo sac that protrudes from the micropyle into the cavity between the funiculus and placenta. As embryo sac is mature, the egg cell, the two synergids and about half the central cell are located outside the micropyle.

蓝猪耳在其发育的四核期，胚囊从珠孔端伸出，当胚囊完全成熟时，助细胞、卵细胞和大部分中央细胞均暴露于胚珠组织之外，是研究被子植物受精和胚囊发育的模式植物。

In resting cysts, the microtubules under the pellicle, cortical ciliatures, conjunctions between cilia basal bodies, supporting structure of basal bodies and other microtubule structures are absorbed in different extent; microtubule-like assemble bodies appear at the center of the remained cilia basal bodies; the granules of chromatin and nucleolus in macronucleus become smaller whereas nucleus pores of the micronucleus become bigger; the inner membrane of some nucleus pores are attached with chromatin.

结果表明：纤毛虫形成包囊过程中，皮层细胞质中产生具有自噬泡消化特征的细胞器，将部分纤毛结构等逐步消化；休眠包囊中，表膜下微管、皮层纤毛器和纤毛基体间连接、基体托架及其他微管结构不同程度地被吸收、在残剩纤毛基体的中央形成微管形结构聚合体，大核染色质和核仁颗粒变小，核孔增大，并发生染色质附着于核孔内膜的现象；细胞脱包囊期间，皮层细胞骨架、线粒体等经历了在残剩结构基础上的再分化。

It was found that the process of secretion can be divided into three phases in rat gonadotropes and pancreatic β cells. The three phases correspond to rapidly releasable pool , slowly releasable pool and unprimed pool , respectively.

在大鼠促性腺激素细胞和胰腺β细胞上观察到，它们的分泌都可分为三个阶段，这三个阶段分别对应于快速分泌囊泡池、慢速分泌囊泡池和未启动囊泡池。

After selective extraction, the cyst wall of two kind of resting cysts displayed a filament network. In the resting cysts of Tachysoma pellionella and Euplotes encysticus, the filament was located in mesocyst and endocyst respectively. On the other hand, a special filament scaffold region was observed below the cyst wall in two kind of cysts. Compared with the true IF which was located in central part of cyst, the filament of this region was net as well as uniform, and no any cytoplasm residue can be seen in this region. In the resting cyst of Euplotes encysticus, a filamental boundry which partitioned the special filament scaffold region and the true IF region was observed.

两类休眠包囊的包囊壁经生化分级抽提后均显示纤维结构，急纤虫休眠包囊中纤维定位于中间层，包囊游仆虫休眠包囊中纤维定位于内层壁；其次，在两类休眠包囊的包囊壁下，相当于细胞质表质层的位置，均有一个特殊的纤维网络区，与细胞质深层的网络区相比，此部分的纤维纯净、均匀，未见附着细胞器及细胞质残留物，在包囊游仆虫休眠包囊中此纤维网络区与真正的细胞质纤维网络之间由纤维构成的界膜隔开。

4Histological examination of the capsular bags: It could be seen in group 4 that a small amount of LECs at the capsular equator proliferated and migrated to the posterior capsule, forming a thin soemmering ring in the periphery of the capsular bags, forming thin fibromembrane which embodied monolay metaplastic fibroblast-like cells in the center of the capsular bags, or with no cells or cortical material in the center of the capsular bags.

囊袋组织学检查：HSV-TK+GCV组可见少量赤道部晶状体上皮细胞(lens epithelial cells， LECs)增殖并向后囊膜迁移，在囊袋外周部形成稀薄的soemmering环，在中央部无细胞和皮质，或仅形成单细胞层纤维膜。

Methods Neomycin was used to destroy guinea pig utricular hair cells in vitro.The culture in experimental groups was supplemented with bFGF.The culture in control group was not supplemented with bFGF.All utricles were observed by scanning electronic microscope,and the number of survival utricular hair cells was accounted.

采用离体的豚鼠椭圆囊培养法，对照组为普通前庭培养液培养，实验组按培养液中含和不含bFGF分组，用新霉素造成各实验组培养的椭圆囊毛细胞损伤，各组椭圆囊均行扫描电镜检查并行毛细胞计数。

The LBL self-assembly process was monitored by flow cytometry and zeta potential analyzer. Scanning electron microscope, dynamic light scattering, and flow cytometry were used to characterize microcapsules The microcapsules were 1.14 μm in size and stable in lyophilizing.

在微囊的制备过程中，采用流式细胞仪和Zeta电位仪对层层自组装进行监测，并对制得的微囊采用扫描电子显微镜、动态光散射仪、流式细胞仪等进行表征，结果发现微囊的直径在1微米左右，尺寸较均一。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本试验以昆明小鼠1-细胞期胚胎为实验对象，以CZB培养液为基础液在不同发育阶段添加外源性ROS，培养至桑椹胚、早期囊胚、囊胚、扩张囊胚阶段，分别观察胚胎的胚细胞数、分裂指数的变化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。