细胞囊

Structural and envelope glycoprotein E2 (gp55) of Classical swine fever virus is the most antigenic protein being responsible for eliciting neutralizing antibodies and conferring protective immunity. Infection of cells with CSFV is mediated by the interaction of E2 and Erns with the cell surface receptor.

猪瘟病毒囊膜表面结构糖蛋白E2 (gp55)是诱导机体产生中和抗体及激发保护性免疫应答的主要抗原蛋白。E2和Erns与细胞表面受体的相互作用介导病毒对细胞的感染过程。

Detail Contents: Genetic disorders -- Immune deficiencies -- Breast cancer -- Colon cancer -- Melanoma -- Cystic fibrosis -- Hemophilia -- Liver disease -- Cardiovascular disease -- Muscular dystrophy -- Alzheimer's disease -- Parkinson's disease -- Huntington's disease -- Viruses: the cornerstone of gene therapy -- Viruses are living crystals -- Viral genomes may be RNA or DNA -- Viruses evolved from plasmids -- Viruses know how to infect cells -- The virus as a gene vehicle -- Viruses used in gene therapy -- Ashi DeSilva: a promising start -- Clinical trials defined -- Cells of the immune system -- Adenosine deaminase -- Preliminary research -- Clinical procedure for ADA gene therapy -- The DeSilva clinical trial -- Jesse Gelsinger: down to earth -- Ornithine transcarbamylase -- Preliminary research -- Clinical procedure for OTC gene therapy -- The Gelsinger clinical trial -- The investigation -- Concluding remarks -- Future prospects -- Safer vehicles -- Reducing immune rejection of the vector -- Improved risk assessment -- Redesigning human anatomy and physiology -- Ethics of gene therapy -- The Belmont report -- Clinical trials -- Physiological enhancement -- Cosmetic applications -- Legal issues -- Regulatory agencies -- The Gelsinger legal trial -- International regulation -- Resource center -- Eucaryote cell primer -- Recombinant DNA primer -- The human genome project -- X-linked severe combined immunodeficiency (SCID-X1)-- Alzheimer's disease -- Huntington's disease.

细节内容︰遗传疾病-免疫的缺乏-乳腺癌-结肠癌-黑瘤-囊性纤维变性-血友症-肝疾病-心血管疾病-肌营养不良-早老性痴呆病-帕金森疾病-亨廷顿疾病-病毒︰基础的基因治疗-病毒在活著水晶--病毒的基因可能是RNA或者DNA --病毒从plasmids被逐步形成--病毒知道怎样感染细胞--作为一辆基因车辆的病毒--基因治疗使用的病毒-Ashi DeSilva︰有希望开始-临床试验确定--细胞的这免疫系统-Adenosine deaminase-初步研究-临床程式给埃达基因治疗--这DeSilva临床试验-婕西Gelsinger︰到地球-Ornithine transcarbamylase-初步研究-临床程式给OTC基因治疗-- Gelsinger临床试验-调查-达成评论-前景-更安全的车辆--矢量的降低免疫的拒绝-改进风险估计-重新设计人解剖学和生理学--伦理学的基因治疗-那些贝拉蒙特报告-临床试验-生理提升-美容应用-法律问题-协调机构-- Gelsinger 合法审讯-国际管理-资源中心人物-Eucaryote信元第一-Recombinant DNA 入门--人类基因工程-- X 连结的严重的结合的免疫缺陷(SCID-X1)-早老性痴呆病--亨廷顿的疾病。

In recent years,small G proteins have become an intensively studied group of regulatory GTP hydrolyses involved in cell signaling, which take part in numerous and diverse cellular processes, such as gene expression, cytoskeletal reorganization ,microtubule organization, and vesicular and nuclear transport, functioning as molecular switches.

另外，小G蛋白的调控途径近年来己经成为人们研究细胞信号转导过程的热点，它在基因表达、细胞骨架重组装、微管的形成以及囊泡和核孔的运输机制中，起着一个分子开关的作用。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

We used the medaka as a fish model for the development of ES cell technology. We have established feeder cell free culture conditions and obtained several ES cell lines from midblastula embryos.

我们以青鱂作为建立鱼类ES细胞技术的模式，通过建立并应用无滋养层细胞的培养条件，获得了来自中期囊胚的ES细胞系。

Methods The B6D2F1 hybrid mouse oocytes were parthenogenetic activated with strontium chloride(SrCI2) and cytochalasin B. The parthenogenetic blastocysts and morulas were seeded on MEF feeder respectively to examine the primary ICM appearance.and compare the derivation rate.

采用氯化锶联合细胞松弛素B激活B6D2F1杂交小鼠卵母细胞，所获得的囊胚与桑椹胚分别用于孤雌胚胎干细胞的建系，观察两者的建系成功率。

In order to fuse, in tracellular vesicles have to move with in the cell

为了融合，细胞内的囊泡必须在细胞内运动。

Results: HL-60 cells＇ growth was obviously inhibited after induction with different density arsenic trioxide; autophagy vesica was observed using fluorescent microscope; Bcl-2 gene expression was inhibited; cells autophagy level was negative correlated with Bcl-2 gene expression.

结果：经不同浓度As2O3作用后，HepG-2细胞生长明显受到抑制；荧光显微镜可观察到自噬囊泡的出现；Bcl-2基因表达降低；经相关性分析，HL-60细胞自噬水平与Bcl-2基因表达呈负相关。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

The continuous ethanol fermentation with mixed substrates consisting of glucose and xylose in two series-wound fixed columns packed with gel beads of immobilized cells was developed and tested in the experiment.

本研究采用海藻酸钙固定普通酿酒酵母细胞和嗜鞣管囊酵母细胞于两个串联的发酵罐内，连续发酵葡萄糖和木糖组成的糖液并与膜耦合来制取酒精。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。