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细胞囊

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The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明: 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h,胞质突起率和去核率没有显著的差异,突起率可高达85.4%,去核率达到100%; 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h,对去核效果没有显著影响; 3 对于体外成熟18~23 h的卵母细胞,随着成熟时间的延长,盲吸法的去核率降低,但没有影响秋水仙素诱导胞质突起的比率和去核率; 4 两种去核方法对重构胚的发育没有产生显著影响,但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

METHODS: A computer-based online search of PubMed database was undertaken to identify related articles about the microcapsulated cell or encystation material for treating diabetes mellitus published from 1980 to 2009 by using the key words of "microcapsulation, islet, transplantation" in English. Meanwhile, we search CNKI for relevant articles published between 1999 and 2009 with the same key words in Chinese.

检索PUBMED数据库中1980/2009有关人工细胞微囊技术进展在糖尿病中应用的文献,检索词"microcapsule, islet, transplantation",并限定文献语言种类为English;同时检索中国期刊全文数据库1999/2009有关人工细胞微囊技术进展在糖尿病中应用的文献,检索词为"微囊,胰岛,移植"。

Results MRI findings of central neurocytoma are specific in 5 cases. The ganglioglioma was found in the tempora and occipital lobe in 7 cases,in the brainstem in 1 case, cystic-solid tumor was observed in 5 cases with extramural nodule in 3 cases, obviously thickened gyri were revealed in 1 cases and central necrosis in 2 cases.The 3 cases of Lhermitte-Duclos disease include the specific molecular layer imaging features.

结果 5例中枢神经细胞瘤有一定的MRI特征,3例诊断正确。8例神经节细胞胶质瘤中,7例位于或主要位于颞、枕叶,1例位于桥脑和延髓,表现为囊实性者5例,实性部分位于囊外的有3例,呈实性表现为局部脑回增厚者1例,囊变坏死明显者2例。3例小脑发育不良性节细胞瘤有特征性的条纹状或分层状表现。

There are significantly differences on H_2O_2 levels between embryos in vivo and cultured in vitro on the early 2-cell stage and late 2-cell stage, but there are no significantly differences among embryos those on the 4-cell stage, morula and blastula using SPSS statistics software.

我们测量的结果是体内2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是52.92,50.77,48.84,39.96和30.36;体外2-细胞早期、2-细胞晚期、4-细胞期、桑椹胚、囊胚胚胎的EB荧光密度分别是74.85,82.62,82.98,81.28和54.09。

Plants involved in this association are most of the terricolous plants. The fungi related are classified in six genera of order Glomales, they are Glomus, Sclerocystis, Acaulospora, Entrophospora, Gigaspora and Scutellospora.

参与丛枝菌根形成的植物是大多数的陆生植物,参与丛枝菌根形成的真菌是隶属于球囊霉目6个属的真菌,即:球囊霉属、硬囊霉属、无梗囊霉属、内养囊霉属、巨孢囊霉属和盾巨孢囊霉属,它们共同的典型特征是在宿主根皮层细胞中能够形成树状的分支,即丛枝。

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同,滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%,显著高于昆明鼠(47.6%);自发激活率分别为13.3%和46.0%,C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%,显著低于SrCl_2孤雌激活率(96.4%);自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%),但囊胚率(18.7%)较低,同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%,差异不显著。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组,设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植,囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性,共8天,进行生长曲线分析。

Megaspore development and abortion of"Pingyi Tiancha"Compar-ing with regular developing megaspore of normal M.baccata,the megasporemother cellof"Pingyi Tiancha"could form mono-nucleus embryo sacby perhaps irregular meiosis,then most of sexual MES depauperatedand degenerated,finally aborted,or only a few further developed.Of asexualembryo sac,one or more large cells at the chalazal end of nucellus developed in-to one to more asexual embryo sac initials.After the competition between sexu-al embryo sacand asexual embryosac,most ovules only had one asexual embryo sac matured.

平邑甜茶大孢子的发育和败育与正常发育的山定子大孢子对比,平邑甜茶大孢子母细胞经减数分裂能形成单核胚囊,之后多数有性胚囊萎缩、退化而败育,只有极少数进一步发育;而无性胚囊则从胚珠合点端1个或多个较大的珠心细脆形成并首先发育为一个或多个无性胚囊原始体;经过有性胚囊和无性胚囊以及无性胚囊和无性胚囊之间的竞争,多数胚珠只有一个无性胚囊成熟。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法:利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变,使编码子249由精氨酸变成丝氨酸,然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中,将注射过的囊胚植入假孕的雌性小鼠子宫,到第14d取小鼠胚胎纤维母细胞,用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞,经测序证实细胞含有由249Arg到Ser的突变。

RESULTS: In the verapamil group, the fibroblast were round or oval, and there were tiny cell process, less alpha substance, atrophic and decreased rough endoplasmic reticulum in kytoplasm and abundant vesica. In control group, there were fusiform or polygon fibroblast, and much matrix among cells, abundant rough endoplasmic reticulum and ribosome in cytoplasm and less vesica were found.

结果:维拉帕米组神经瘢痕成纤维细胞呈球形或椭圆形,细胞突起细小,胞间网状物质少,胞浆中粗面内质网萎缩且数量减少,囊泡丰富;对照组成纤维细胞呈梭形或多角形,细胞间基质较多,细胞浆内粗面内质网及核糖体丰富,囊泡少见。

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