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An expert on Netherlands flora, De Vries showed that turgor pressure of the fluid in plant cells was partially responsible for growth and coined the term ' plasmolysis ' for the effect on cells of water loss during wilting.

作为研究荷兰植物群的一位专家。他发现植物细胞中液体的膨胀压力在细胞由于缺水而萎蔫时可以使细胞发生质壁分离。

The stem was cut into several segments away from the pine seeding, arranged and collected in the order of chronology time and distance from where they were inoculated. To separate B.xylophilus coarsely out from the stems and made them into paraffin slices. And be observed under the potics microscope by dyed with safranin-fast green contrast-stain method and PI fluorescence stain method. In order to find out how about the nematode rigour distributing , but also ebb and flow of their amounts after invading the host pine seeding. Seek the pathological changing orderliness of host cells in different position along with the remotion of nematodes. By deeply analysing the variety of host cell nucleolus to estimate whether the host cell took place the programmed cell death after inoculated B.xylophilus.

按时间进程和与接种点上下距离的远近,对松苗分茎段进行粗分离和制作成石蜡切片,分别用番红-固绿双重染色法和碘化丙锭荧光染色法在光学显微镜下观察,以了解线虫侵染后在寄主体内的准确分布和数量的消长,寻找随着线虫的移动扩散,寄主不同部位细胞病变的数量、速度发生的变化规律,并进一步通过分析其细胞核的变化来判定松材线虫侵染后是否能诱发寄主细胞发生程序性死亡(programmed cell death,PCD)。

In Dictyostelium amoebae, cell-type differentiation, spatial patterning, and morphogenesis are controlled by a combination of cell- autonomous mechanisms and intercellular signaling.

在变形虫盘基网柄菌中,细胞自主机制和细胞内信号的共同作用控制着细胞类型的分化、空间模式和形态发生。

Cell apoptosis is an important factor in the cell necrose following the skeletal muscle damage. The apoptosis relative genes such as Bcl-2, Bax are crucial in skeletal muscle cell spoptosis. Adaptation and damage are two dichotomal facets in daily physical exercise and military training.

细胞凋亡是骨骼肌损伤后细胞发生坏死过程中的重要作用因素,如凋亡相关基因:Bcl-2、Bax等在骨骼肌细胞凋亡过程中起着非常重要的作用。

Before fissuration, osmotic adjustment occurred by the addition of solute to cells and decreasing the loss of water in the body, so that it could maintain normal turgor pressure during drought. After fissurate growth, turgor pressure was maintained by higher cell and tissue elasticity lower ε(superscript max and by osmotic adjustment for normal plant development in dry conditions and to avoid dehydration under water stress during the drought period, but it also became more sensitive to water stress. The ability to maintain turgor pressure varied from strong to weak during the period before, during and after fissuration. Fissurate growth decreased the ability of P. mongolica to maintain turgor pressure and led to changes in walls related to drought tolerance.

结果表明:1未劈裂绵刺主要通过增加细胞内溶质,减少细胞内的水分丧失来进行渗透调节,从而在干旱胁迫下能够维持正常的膨压。2己劈裂绵刺通过渗透调节和高的组织弹性两条途径来共同保持膨压,以抵抗不良的生存环境;同时对环境水分胁迫具有较高的敏感性。33种状态绵刺保持膨压的能力由强到弱依次为:未劈裂绵刺、正在劈裂绵刺、已劈裂绵刺。4劈裂的发生导致绵刺保持膨压能力的降低,同时耐旱方式和途径发生了变化。

This effect was observed clearly at final concentration between 1 and 10μ mol/L after treating for 24 hours. We concluded that the possible mechanisms of hydroquinone bleaching hyperpigmented skin were through its inhibition of tyrosinase activity at lower concentration and its cytotoxicity at higher one.

提示18α-甘草酸双胺盐、熊果苷的皮肤脱色作用机制与氢醌可能不同,后者低浓度时以抑制酪酸酶活性为主,高浓度时主要是细胞毒,且细胞早期的损伤部位可能发生在树状突。18α-甘草酸双胺盐能否成为一种无细胞毒酪氨酸酶抑制型皮肤脱色剂有待进一步临床加以验证。

Results Nicotinamide at 1-5 mg/ml induced apoptosis of GLC-82 cells in a dose-dependent manner, and group 4 mg/ml and group 5 mg/ml showed increased apoptosis than other groups. Distinct morphologic changes of cell apoptosis such as karyopyknosis and conglomeration were observed; Cell migration became slower as the dosage of Nicotinamide increased;Cells produced an arrested at G1 phase, accompanied by a reduction of cells transiting through S phase.

结果 烟酰胺诱导细胞凋亡程度与用药剂量呈正相关,较高剂量的药物能引起GLC-82肺腺癌细胞呈现边界不清,体积缩小,核固缩,染色质凝集、边缘化,甚至细胞发生破碎,凋亡小体形成;随着烟酰胺浓度的增高GLC-82细胞向划痕区的愈合速度不断减慢;烟酰胺的浓度大于等于3 mg/ml 时,细胞周期主要表现为G1 期比例的上升伴随S期比例的下降。

Supervital NBS staining Nile Blue Sulphate , a lysosomotropic vital dye which stains embryonic apoptotic, but not necrotic cell death, is employed to identify the occurence and location of apoptosis.

活体胚胎的NBS染色 NBS是一种亲溶酶体染料,可使凋亡细胞着色,而坏死细胞不着色,因而可初步判定细胞凋亡发生的区域和程度。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2~3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12,在酵母细胞中实现了多拷贝强表达;荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液,测定510 nm处的荧光发射强度结果显示,表达的绿色荧光蛋白对氧化还原水平敏感,且在510 nm处的荧光强度与一定的重金属浓度呈正相关,即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强,从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用;同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属;遗传学的点突变频率及致死率实验数据表明,重金属能导致菌体的点突变频率和致死率升高,且活性氧的清除剂巯基脲能明显降低这种点突变和致死率,说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

While in another 8 DRG C cells the depolarizing responses to morphine were potentiated, in both amplitudes and courses, by administration of CA- DO.

结果提示:吗啡可引起多数C类DRG细胞发生明显的去极化效应,而只对少数A类细胞有轻度超极化作用,但甲—脑啡肽对DRG细胞的膜电位作用不大。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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然而,要让一个真正的引用,你需要提供详细的个人和财务信息。