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The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物,从猪卵巢上获得的卵母细胞数量虽然较多,但成熟度比较低;由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内,跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应;如果卵胞质成熟或活化程度不够,加之圆形未成熟精细胞成熟度比较差等原因,很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足,引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等,使ROSI显微受精失败。

It was observed that cellular contents, including chromatins and organelles,migrated in PMCs through cytoplasmic channels,mainly at the stage of synizesis in meiotic prophase Ⅰ.

观察发现,处于凝线期的花粉母细胞,其内含物,包括细胞器和染色质,主要通过胞质通道向相邻细胞发生转移。

The podocyte is a highly differentiated interdigitating cell,theist iniury is closely associated with the genesis and development of proteinuria,This article reviews manifestations,mechanism and the treatment of podocyte injury.

足细胞是一种终末分化的多突状细胞,足细胞的损伤与蛋白尿的发生、发展密切相关,现通过对常见足细胞损伤的表现,及针对足细胞损伤的机制和治疗予以综述。

Thpetum is the gland cell type. The pistil has two carpels, the ovules belong to straight ovules with bitegmic;77% nucellus stoped development before from the archesporial cell and disappear and 23% nucellus atrophy .so the flower cannot grow seeds.

安祖花的雌蕊是由2 心皮组成,胚珠为直生胚珠,双珠被,77%珠心细胞在胞原细胞形成之前停止发育,并且逐渐解体消失,23%珠心细胞发生萎缩,导致雌蕊败育。

It is well known that retinal pigment epithelial cells are predominant proliferative cells in PVR which is an excessively wound healing response occurring after RPE cell wounding. The activated RPE cells migrate from their normal, sessile state to vitreous and onto both surfaces of the retina, in which they begin to dedifferentiate, migrate, proliferate, change phenotype and secrete ECM.

既往研究证明视网膜色素上皮(retinal pigmentepithelial,RPE)细胞是参与PVR的主要细胞,PVR是视网膜脱离后RPE细胞损伤的一种自我修复过程,是一种过度的眼内创伤修复反应;RPE细胞在正常位置上处于静止状态,而在某些病理条件下,如视网膜裂孔形成和视网膜脱离后,RPE细胞脱离原位,开始去分化、移行、增生、发生表型转化并分泌胶原等ECM,最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜,造成牵拉性视网膜脱离。

One histiocytic sarcoma showed t(14;18) by quantitative-polymerase chain reaction, which was confirmed by fluorescence in situ hybridization analysis showing IGH/BCL2 fusions in neoplastic histiocytes.

定量性聚合酶链反应显示1例组织细胞肉瘤发生了t(14;18),此结果也得到荧光原位杂交分析的证实,即肿瘤性的组织细胞发生了IGH/BCL2基因的融合。

This process, called vasculogenesis ,involves the de novo differentiation of endothelial cell from mesodermal precursor to form the primitive blood vessel network.

血管生成是指在胚胎发生和器官形成过程中,从中胚层内皮细胞中分化出新的内皮细胞以形成原始血管网的过程,仅见于胚胎发生的早期,出生后即停止。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.[Key words]astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异,发现ApoD在2型星形胶质细胞中差异高表达,故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况,以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据,依此探讨胶质细胞发生和分化的信号机制。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究结果表明:(1)昆明白小鼠与衰老相关的生育力下降是其卵母细胞数量减少和质量下降的综合结果;(2)年轻小鼠卵母细胞细胞质不能纠正老龄小鼠GV的减数分裂错误,老龄小鼠卵母细胞细胞质也不诱导年轻小鼠的GV发生减数分裂错误,老龄小鼠卵母细胞减数分裂异常很可能与细胞核或染色体及其相关因素有关;(3)关键的核质比对卵母细胞正常的成熟、减数分裂染色体分离及2-细胞期胚的发育是绝对必需的。

Examination the cell shape and pseudopodia with confocal laser scanning microscopy, scanning electron microscopy, and atomic force microscopy, we found that cell spreading well on nanogrooved substrates and their body was more flat and slim; meanwhile, on plain substrates, cells were mostly round.

纳米沟槽能很好地促进细胞贴壁黏附、铺展、生长和增殖,并影响细胞的周期;纳米沟槽诱导细胞骨架发生重组,肌动蛋白骨架沿着纳米沟槽的方向取向排列;纳米沟槽影响细胞伪足形成的方式和形成与细胞中的位置。

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