细胞发生

Marrow stromal cell ; fibrin sealant ; cell culture ; bone morphogenic protein ; basic fibroblast growth factor

骨髓基质细胞；纤维蛋白胶；细胞培养；骨形态发生蛋白2；碱性成纤维细胞生长因子

We have previously showed that CD40 activation induced the cell fusion and formation of multinucleated giant RS like cells from B lymphoma Daudi cells, which makes it clear that the Daudi cells obtained the ability of invasion after CD40 triggering.

CD40分子激发后的恶性B淋巴母细胞Daudi发生自身融合现象，表明肿瘤细胞在CD40分子激发后获得了某种侵润能力。而恶性B淋巴细胞的特性之一就是肿瘤细胞的广泛转移和侵润。因之，我们推测淋巴细胞的CD40分子激发可能对其扩散和转移会产生影响。

AQP 1 has been found associated with nephradenoma, breast cancer, cholangiocellularcarcinoma and hemangioblastoma of CNS.

现已发现AQP1表达于肾腺瘤、乳腺癌、肝脏胆管细胞癌、中枢神经系统的血管母细胞瘤，AQP2表达于肾细胞癌，AQP3的检测有助于肾集合管癌的诊断和鉴别诊断，AQP5可能参与卵巢肿瘤的发生。

Western Blot indicated that AIF was selectively released before cytochrome C from the intermembrane space of mitochondria to cytosol, and that release needed Bax relocation to mitochondria. Results above suggest that caspase-independent cell death maybe occurred earlier than caspase-dependent cell death in staurosporine induced T lymphocytes apoptosis. Caspase-independent cell death is an alternative pathway of cell death which could guarantee the safe and noninflammatory removal of corpses when caspase was mutented or blocked in the cell, it plays an important role in maintaining the number of T lymphocytes and ensuring the proper function of immune system.

细胞caspase非依赖死亡方式可能是Caspase依赖性死亡方式的替代途径，它可以保证在Caspase发生突变或通路受阻时，T细胞仍然能够正常死亡，这对于维持T细胞的数量和保证免疫系统正常功能的发挥具有十分重要的意义。

The appearance of DNA ladder from the ovules in which pollen chamber formation was undergoing, together with TUNEL assay and ultrastructural changes, demonstrated that the nucellar cell death, doomed to bring about pollen chamber, was a process of programmed cell death .

在贮粉室的形态发生过程中，相关珠心细胞的DNA电泳出现DNA ladder、其细胞核被TUNEL反应所标记以及它们的超微结构变化都说明，参与贮粉室形成的珠心细胞的死亡是一种细胞程序性死亡（programmed cell death，PCD）。

Methods The expression of caspase-3 and PCNA was detected by tissue microarray and immunohistochemical method in 95 hepatocellular carcinoma tissues,68 parahepatic cancer tissues and 19 normal liver tissues.

目的 探讨Caspase-3和PCNA在肝细胞肝癌的表达及其在肝细胞肝癌发生、发展中的作用方法应用组织芯片技术和免疫组化方法检测95例肝细胞肝癌组织、68例癌旁组织和19例正常肝组织中Caspase-3和PCNA的表达。

BACKGROUND: The essence of cell differentiation is a selectively intra-cellular gene expression, which results in specific proteinic synthesis and causes changes in biochemistry, structure and function. Thus, original proteomics and a single protein analysis can not meet the requirement in study.

摘要背景：细胞分化的实质是细胞内基因的选择性表达，导致细胞新的特异性的蛋白质的合成，从而在生化、结构及功能上发生变化，原有的基因组和分析单一蛋白的模式已不能满足研究的需要。

Results bFGF 2.5ng/mL promoted ECV-304 cell migration. Cell pseudopodia was increased and cell skeleton was rearranged.

结果 bFGF 2.5ng/mL促进ECV-304细胞迁移，迁移细胞突起增多，细胞骨架发生重排。

According to the previous results, BNIPL-2 could interact with Cdc42GAP and suppress the GAP activity of Cdc42GAP toward Cdc42. BNIPL-2 also had a BCH domain (BNIP-2 and Cdc42GAP homology), which could induce short and long pseudopodia, and subsequently was needed to trigger cell migration. So we presume BNIPL-2 could be involved in cell migration and cancer metastasis.

前期实验发现，BNIPL-2可以和Cdc42GAP发生相互作用，并且抑制Cdc42GAP的活性；而且BNIPL-2中有一个重要的结构域：BCH domain，它可以诱导细胞伪足的产生，从而参与调节细胞的运动，提示了BNIPL-2可能参与细胞的运动和肿瘤转移。

As results, TEM showed characteristic ultrastructure morphology of apoptosis cell with chromatin marginal condense, nucleus pycnosis, mitochondrion vacuoles and cell shrinkage in IR injury group and no apoptosis cells in control group. Increased TUNEL-positive cell and typical DNA laddering were found and cells apoptotic rate were approximately 20% by flow cytometry assay in experimental groups.

值得探讨。本试验通过流式细胞仪、生物电镜、TUNEL法、RT-PCR以及DNA电泳等试验方法研究了带血供同种异体骨移植过程的细胞凋亡现象，并检测了Fas、FasL、Caspase-1、Caspase-3、Bcl-2等凋亡相关基因在IR损伤时的表达及变化情况，初步探讨了骨移植时IR损伤中细胞凋亡的发生机制及其在IR损伤中的生物学意义，希望为今后更深入的研究打下一定基础。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。