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Objective To study the antigenpresenting function of splenic dendritic cells in mice after hemorrhage plus closed fracture.Methods Splenic DCs were isolated 24h after hemorrhage plus closed fracture,then the ability to stimulate nonadherent splenocytes proliferation in the presence of conalbumin in vitro was detected,IL12 and IL10 levels in cell supernatants were measured by ELISA kits.

大量切磋证明,创伤能引起机体免疫编制发生庞杂的改变,树突状细胞(dendritic cells,DC)是体内效用最强的专职抗原递呈细胞(antigenpresenting cells,APC),它可摄取和加工抗原,并渗透多种细胞因子,在激励T 细胞的免疫应答中起重要作用。

It is well-known that for eucaryotic cells, DNA is located in the karyon and can not penetrate to the cytochylema and the tissue fluid because the cell membrane can act as barriers for DNA transportation. Therefore, the appearance of DNA in cell matrix and the serum can indicate that some diseases have taken place. A main reason for cancer occurrence is the mutation of DNA. It may cause the change of the sequence and expression of cancer suppressor genes.

真核细胞的DNA广泛的存在于细胞核内的染色体上,由于核膜的选择性通透作用,使DNA无法分散到其它细胞的亚器官,加之细胞膜的隔离效果,DNA更难扩散到细胞外基质中,因此细胞外基质中DNA含量的增加往往也会预示某些疾病的发生。

GSK-3βis now recognized as not only a key rate-limiting enzyme of glycogen metabolic process, which phosphorylats hepatic glycogen synthase and makes it devitalize by insuline regulation, but also plays an important role in at least three signal transductory systems, namely, the Wnt/wingless, PI3'-kinase and Hedgehog pathways,which influence cellular processes, such as protein synthesis, cell proliferation, cell differentiation, cell motility and apoptosis.

GSK-3β是糖原代谢过程中的重要限速酶,除了可以在胰岛素调控下磷酸化肝糖原合成酶并使之失活外,GSK-3β还在多个重要信号通路中扮演了关键角色,例如Wnt/β-catenin pathway,PI3K/AKT pathway,Hedgehog pathway,在蛋白合成、细胞增殖、细胞分化、细胞运动、细胞凋亡以及肿瘤发生等方面都扮演了重要角色。

In the gravid uterus, The CKs immunolabelling were detected in glandular cell, luminal epithelial cell, traphoblast cell, endoblastic cell and allantoic cell; Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus but in endoblastic cell and some luminal epithelial cell.

对分离得到的子宫内膜基质细胞和子宫内膜腺上皮细胞进行免疫组织化学标记的结果显示在体外子宫内膜基质细胞表达泛角蛋白,子宫内膜腺上皮细胞表达波形蛋白,并且这一特性不因为传代而发生丢失。

Paraffin sectioning was employed to study the developmental structure of stems and leaves of Messerschinidia sibirica, especially the position, the structure and the formation and development of the salty gland. The result indicated: that salty gland of M. sibirica is from epidermic cell of stem and leaf. Based on the observation we found one primary kind of salty glands structure which consists of many cells on the epidermis including the collecting cell and the secretory cell and so on.

利用石蜡切片法研究了砂引草茎叶的解剖结构,尤其是对泌盐腺的位置、泌盐腺的形态结构及泌盐腺的发生发育过程进行了系统的研究,结果表明:砂引草的盐腺由茎叶的表皮细胞发育而成,通过观察发现在砂引草的表皮上存在着一种主要的盐腺结构,它是由多个细胞组成,包括收集细胞和分泌细胞等。

Its pathologic mechanism has the close relation to high blood sugar, the excrescent of kidney blood dynamics and the household hereditary, etc. Following the improvement of molecular biology, in recent years, many people discovered that many varieties of cell factors take part in the process of DN and exertive important functions.

近年来随着分子生物学技术的进步,研究发现有多种细胞因子参与DN的发生发展过程,其中以转化生长因子、血小板衍化生长因子为核心因子,其作用涉及到肾小球血流动力学改变、细胞外基质代谢、细胞增殖和细胞肥大等诸多方面。

After treated with the samples, the MGC-803 cells are dyed with the fluorescence dye Hoechst 33258. Observed by the fluorescence microscope, the dense dyeing granulometric and nubby blue fluorescence can be seen in the nucleus of MGC-803 treated with AK-9 and AK-11, the nucleus of some cells smash to small fragments.

采用荧光染料Hoechst 33258对经初筛活性样品处理的MGC-803细胞染色,荧光显微镜观察,发现经AK-9及AK-11发酵液处理的细胞,其细胞核内可见浓染致密的颗粒或块状荧光,细胞核发生固缩、碎裂,而对照细胞的细胞核呈均匀弥散荧光,表明这2株菌株可能产生具有诱导细胞凋亡的活性物质。

To a certain degree, there may be cellular immunodeficiency existing in patients with hypopharyngeal squamous cell carcinoma.

背景与目的:T淋巴细胞亚群及NK细胞是主要的细胞免疫形式,通过对两者的研究可以了解细胞免疫在肿瘤发生、发展的作用、喉咽鳞癌患者可能存在一定程度的细胞免疫缺陷。

Spontaneous germ cell death is very important for the removal of germ cells and control of the quantity and quality of sperm. But excessive germ cell death will lead to diminish spermatogenic function and decrease the quality and quantity of sperm. Excessive germ cell death triggered by various regulatory stimuli, including deprivation of gonadotropins and intratesticular testosterone or by estradiol treatment in rats via apoptosis.

生精细胞凋亡是哺乳动物精子发生过程中的一个显著特点,其生理性凋亡对于去除受损的生精细胞,控制细胞数量和保证精子的质量具有重要意义,但生精细胞的过度凋亡可引起生精功能低下,使精子的数量和质量降低。

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ( P .05). There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%(48h after transfection) to 5.63%(72h after transfection).

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制( P 。05);细胞形态发生改变,细胞体积缩小,核固缩,染色质浓缩聚集于核膜;流式细胞术检测显示G 1 峰前出现亚二倍体峰,转染48、72h后凋亡率分别为5.47%和5.63%( P nbsp; 0.05)。

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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。