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To elucidate whether overexpression of Nolz-1 may negatively regulate cell proliferation and cell survival during neurogenesis, we performed immunohistochemistry analysis in the developing telencephalon with specific markers of cycling cells and apoptotic cells.

为了阐明过度表现 Nolz-1在神经发生过程中,是否会负向调控细胞增生及细胞生存,我们利用周期细胞和凋亡细胞专一性抗体,在发育端脑中进免疫组织染色分析。

The methods of trophoblast isolation have deficiency of low product ion rate and other cells contamination.

原代培养的滋养层细胞存在产率低、杂细胞污染等方面的缺憾,而滋养层细胞又在细胞特性上发生了变化。

The results showed that there existed variation among eucalypt families in wood physical-mechanical properties and wood chemistry. There were significant differences among E.pellita families、E.grandis families and E.urophylla譋.grandis clones in wood colors as well as between Strains and within trees.The variation of the surface wetability of wood between E.grandis families and E.urophylla譋.grandis clones were significant.Even in the same families level or clones level,there still existed the same regulation. The degree of collapse of eucalypt had a large relation in the amount of tylosis in vessel and vestured pits on the cell wall of vessel. The more rich tylosis and vestured pits in vessels,the more probability to occur collapse for eucalypt.The biggest moment collapse was probably the critical point of the drying degrade.During the fomulation of drying technology,it is necessary to adopt moderate condition to make the vessels which had formed the biggest moment collapse at the biggest moment collapse resume,especially to prevent the permanent set.At the normal temperature level,the main factors which influenced residual collapse were the contents of ray parenchyma and axial parenchyma .Nearly all the cells joined the course of forming the moment collapse and biggest moment collapse.Interval drying was fit for eucalypt plantation which was easier to make collapse.

研究表明:桉树不同家系间木材物理力学性质和化学组成存在差异;粗皮桉家系间、巨桉家系间、尾巨桉无性系间材色存在显著差异,株间、株内木材材色存在变异;桉树木材表面润湿性在巨桉家系间、尾巨桉无性系间存在着显著差异,在同一家系或同一无性系中,株间和株内也存在着相同的变化规律;桉材皱缩程度与其导管中侵填体的多少和导管壁上纹孔具有的附物多少密切相关,侵填体含物越丰富,纹孔附物越多,桉材越容易皱缩;最大瞬间皱缩是能产生更大干燥降等的临界点,在制定干燥工艺时,必须在最大瞬间皱缩发生时,采用温和条件,使已产生最大瞬间皱缩的细胞尽可能多的恢复,尤其不能使其产生永久变定;在常温条件下,影响残余皱缩的主要因子是射线薄壁细胞和轴向薄壁细胞含量;而对于瞬间皱缩和最大瞬间皱缩,几乎所有细胞都参与它们的形成过程;对于易皱缩的桉树木材,间歇干燥是最有前途的干燥方法。

From the tests,we have obtained some structural variant features related with salt resistance and demonstrated the successful introduction of Rhizophora apicularta DNA into tomato.This thesis contains two parts. In the first part,the morphological and anatomical structures of the leaves were investigated. As to the morphology,some difference between control and transformed tomato were found. The leaf dimensions of the transformed tomato with unextended leaves were smaller than that of control. These structure changes should help to decrease the evaporation of water effectively and release the physiological drought resulted from saline.

本实验分两部分:第一部分、对转化番茄的形态及细胞显微结构进行观察与分析,实验表明转化番茄形态与对照相比有明显的变化,其叶面积变小,叶形收拢,这种结构可有效地减少水分的蒸发,缓解由盐胁产生的生理干旱;组织解剖结构结果显示,耐盐番茄在细胞结构上发生了明显的变异:其气孔器倾向下陷,表皮毛增多,叶脉微管组织不发达,微管束周围出现增多的贮水细胞,叶缘及其它部位表面分化出大型的多细胞分泌型结构,这些结构与转化番茄后代较强的耐盐能力有关。

Cardiac hypertrophy is one of adaptive responses of the heart to a variety of pathological stimuli, including hypertension, myocardial infarction, congenital heart disease, heart valvula disease, and perturbations in sarcomeric function due to altered expression or mutations of contractile proteins. Increase in wall tension and hormonal stimuli are thought to directly activate various membrane-bound receptors and signal transduction cascade within cardiomyocytes resulting in the activation of immediate early genes and responsive genes such as c-jun, c-fos, c-myc, egr-1, ANF, BNP. Then the expression of construction proteins such as MLC-1, skeletal α-actin was unregulated.

中文题名hhlim基因的表达调节及其与心肌细胞肥大发生之间的关系副题名外文题名 hhlim gene expression regulation and its role in development of cardiomyocyte hypertrophy 论文作者郑斌导师温进坤韩梅学科专业生物化学与分子生物学研究领域\研究方向学位级别博士学位授予单位河北医科大学学位授予日期2003 论文页码总数106页关键词心肌细胞肥大基因表达心肌肥厚 hhlim基因馆藏号BSLW /2003 /R542.2 /33 心肌细胞肥大是高血压、心脏瓣膜病、急性心肌梗塞及先天性心脏病等临床常见疾病的一种并发症,是心肌细胞对多种病理刺激的一种适应性反应。

According to 5'flank regions and partial sequences of the MyoG gene in Sus scrofa, Bos taurus, Homo sapiens, and Mus musculus published in GenBank, the homogeneous primers were designed to amplify the promoter region of Gansu wapiti MyoG by Touch-Down PCR.

MyoD和MyoG是一类肌调节蛋白,其中MyoD代表肌源性的发生;MyoG诱导骨骼肌的最终分化,调控中胚层细胞分化为成肌细胞,再由成肌细胞融合为肌纤维这一过程,在肌细胞分化过程中起着中心调节作用,是唯一的不可替代的成肌调节因子[2]。

It also indicates that the expression of WT1 is regulated strictly in time and space, and the aberrant expression of WT1 may result in retarded differentiation of blastemal cell leading NR or nephroblastoma.

提示在散发性肾母细胞瘤发生中除WT1突变外,WT1表达延迟可能是其中起重要作用的机制之一;WT1基因与肾母细胞瘤的组织分化有关,可能诱导胚基细胞向上皮细胞方向分化。

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术,对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较,实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常,在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能,并且花粉母细胞减数分裂异常而未形成四分体结构,进而导致雌花雄蕊选择性败育,而雄花中雌蕊组织也发生了选择性败育过程。

The secondary xylem of woody plants is a product of the vascular cambium,its formation is a complicated type of plant cell differentiation, including cambium initiation, cambial cell division, cell expansion, secondary wall deposition and programmed cell death.

木本植物的次生木质部是维管形成层的活动产物,其形成过程是植物细胞分化的一种复杂类型,包括维管形成层发生、形成层细胞分裂、分化、细胞扩展、次生壁形成和细胞程序化死亡等阶段。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实:SMC内固定可对骨折端施加主动的、持续的轴向应力刺激,形成沿长骨骨干轴向的成骨导向力值,配合环抱翼的把持作用,形成了区别于CP、IN的独特的稳定、有效的机械内环境,骨折端的相对稳定减少了不必要的损伤再刺激,同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展,加快了骨折愈合的修复与重建。

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