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In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

Therefore, our data show that in cases when clear cell chondrosarcoma and chondroblastoma pose a diagnostic challenge, the presence of type II collagen in the extracellular tumour matrix significantly supports the diagnosis of clear cell chondrosarcoma and aids in distinguishing it from chondroblastoma.

我们的数据表明,在诊断透明细胞软骨肉瘤和成软骨细胞瘤受阻时,细胞外肿瘤基质中II型胶原的存在对于诊断透明细胞软骨肉瘤相当有帮助,并有助于和成软骨细胞瘤间的鉴别。

A clear cause-and-effect relationship between excessive myocardial MMP-1 activity, increased mysial collagen degradation, and progression to systolic HF has been shown experimentally through the use of transgenic models and the use of pharmacological MMP activators.53,54 In addition, experimental evidence has been provided showing that following chronic neurohormonal activation, increased synthesis and release of MMPs into the local extracellular matrix of the cardiomyocyte occurs,55 which in turn could contribute to endomysial and perimysial collagen degradation and disruption.

一个明显的因果过多的心肌MMP-1关系活动, mysial增加胶原蛋白的降解和进展收缩性心力衰竭已被证明试验,通过使用转基因模型和使用的MMP药理作用此外,activators.53,54实验证据下面被提供显示慢性神经激活时,增加的MMPs合成和释放到当地的细胞外基质的心肌细胞发生,55反过来有助于人肌内膜和 perimysial胶原退化和破坏。

Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein.

用塑料吸头划刮培养皿,制造机械损伤的模型,继续培养至72 h,分别取出不同时间点的细胞进行胶质细胞增生情况的观察和免疫组织化学染色,对胶质原纤维酸性蛋白表达产物光密度进行图像分析。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡,原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化:细胞核固缩,核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘,并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用,与对照组相比有显著性差异(p<0.01),在剂量为0.8-8μmol/L范围内,抑制率随剂量的增加而增加,当剂量超过8μmol/L时,抑制率反而下降;3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性,引起DNA降解损伤,反向调节细胞周期活动,促使细胞从G0期进入G1期,抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核,可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

Here, similarities between archaebacteria and eukarykaryotic otic cells in many aspacts including DNA duplication, transcription, translation and histones, chromatin, proteasome were introduced. Finally, the evolutionary position of archaebacteria and their significance to onery gin of the eukaryotic cells were discussed.

结合国际上对原细菌,尤其是它与真核细胞在许多方面(包括DNA复制、转录和翻译整个中心法则,组蛋白、染色质及蛋白酶复合体等)密切的亲缘关系的研究结果,对原细菌的进化地位及其对揭示真核细胞的起源进化问题的意义进行了探讨。

P<0.01. 28d, q=4. 0452, p<0.05 . Corresponding pathologic result shows that the corneal stromal of height edema increase thickness to have infiltration of inflammation cells with hyperplasia of fibroblast cell and large vessel. The collagen fiber in stromatic layer is dissolved and destroyed seriously, suggesting that in addition to HSV-1's direct destructuring effect, inflammatory cells that might play an important role in the pathology of HSK induce the immune-pathological reaction.

相应的组织病理学研究结果显示(UV-B 14天):病毒对照组角膜基质层高度水肿、增厚,并有大量中性粒细胞等炎性细胞浸润、成纤维细胞和新生血管增生,胶原纤维肿胀、分解等,说明除HSV-1对角膜组织直接破坏外,炎性细胞介导的免疫病理反应在复发性HSK发病机理是重要的因素。

AFGF mitogenic stimulation also results in a decrease in cellular volume and inhibition of fibroblast-mediated contraction of the collagen gel.

aFGF mitogenic激励另外在在胶原质凝胶体的停止纤维原细胞紧缩的细胞的卷和禁止的减少的结果。

Methods: rabbit bone marrow mesenchymal stem cells were purified, and then cultured in inducing medium containing transforming growth factor-β1; mtt assay was employed to evaluate the proliferative activity of induced cells; immunohistochemical assay was used to detect the expression of type ⅱ collagen; induced cells were transplanted into the knee of rabbit, and x-ray photography was employed to observe the repair and formation of cartilage.

纯化兔骨髓间充质干细胞;用含转化生长因子β1的诱导液培养骨髓间充质干细胞;以mtt测定诱导细胞的增殖活性;免疫组化检测ⅱ型胶原蛋白的表达;诱导后的细胞移植于白兔膝关节内,x线摄片观察软骨修复形成情况。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究,解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢,不易形成单层的难题,建立了兔角膜上皮细胞原代培养的方法。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?